Proteintech

CD80/B7-1 Monoclonal Antibody (1E2F10)

CD80/B7-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

CD80/B7-1 Antibody (66406-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 66406-1-IG (CD80 / B7-1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Product Image Gallery

Product Details

66406-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1E2F10

Immunogen

CD80/B7-1 Fusion Protein Ag5615 (36-228 aa encoded by BC042665)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: IHVTKEVKE VATLSCGHNV SVEELAQTRI YWQKEKKMVL TMMSGDMNIW PEYKNRTIFD ITNNLSIVIL ALRPSDEGTY ECVVLKYEKD AFKREHLAEV TLSVKADFPT PSISDFEIPT SNIRRIICST SGGFPEPHLS WLENGEELNA INTTVSQDPE TELYAVSSKL DFNMTTNHSF MCLIKYGHLR VNQT (36-228 aa encoded by BC042665)

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Special issue "The advance of solid tumor research in China": Multi-omics analysis based on 1311 clear cell renal cell carcinoma samples identifies a glycolysis signature associated with prognosis and treatment response.

International journal of cancer

Tian X,Wang Y,Xu W,Tang H,Zhu S,Anwaier A,Liu W,Wang W,Zhu W,Su J,Qu Y,Zhang H,Ye D

Sun Jan 01 00:00:00 UTC 2023

The involvement of Th1 cell differentiation in the anti-tumor effect of purified polysaccharide from Sanghuangporus vaninii in colorectal cancer via multi-omics analysis.

International journal of biological macromolecules

Qu Y,Yang H,Li S,Li L,Li Y,Wang D

Mon May 15 00:00:00 UTC 2023

CTLA4 depletes T cell endogenous and trogocytosed B7 ligands via cis-endocytosis.

The Journal of experimental medicine

Xu X,Dennett P,Zhang J,Sherrard A,Zhao Y,Masubuchi T,Bui JD,Chen X,Hui E

Mon Jul 03 00:00:00 UTC 2023

T cell-mediated targeted delivery of tadalafil regulates immunosuppression and polyamine metabolism to overcome immune checkpoint blockade resistance in hepatocellular carcinoma.

Journal for immunotherapy of cancer

Wang X,Zhang Q,Zhou J,Xiao Z,Liu J,Deng S,Hong X,Huang W,Cai M,Guo Y,Huang J,Wang Y,Lin L,Zhu K

Wed Feb 01 00:00:00 UTC 2023

T cell-mediated targeted delivery of tadalafil regulates immunosuppression and polyamine metabolism to overcome immune checkpoint blockade resistance in hepatocellular carcinoma.

Journal for immunotherapy of cancer

Wang X,Zhang Q,Zhou J,Xiao Z,Liu J,Deng S,Hong X,Huang W,Cai M,Guo Y,Huang J,Wang Y,Lin L,Zhu K

Wed Feb 01 00:00:00 UTC 2023

Cancer medicine

Zhang M,Cao C,Li X,Gu Q,Xu Y,Zhu Z,Xu D,Wei S,Chen H,Yang Y,Gao H,Yu L,Li J

Sun Jan 01 00:00:00 UTC 2023

Macrophages and derived-TNF-α promote lipopolysaccharide-induced upregulation of endogenous β-glucuronidase in the epithelial cells of the bile duct: A possible facilitator of hepatolithiasis formation.

Clinics and research in hepatology and gastroenterology

Yang Y,Wang Y,Wang C,Wu S,Yao D

Sun Jan 01 00:00:00 UTC 2023

Effect of Platycodon grandiflorus Polysaccharide on M1 Polarization Induced by Autophagy Degradation of SOCS1/2 Proteins in 3D4/21 Cells.

Frontiers in immunology

Li L,Chen X,Lv M,Cheng Z,Liu F,Wang Y,Zhou A,Liu J,Zhao X

Tue Jul 19 00:00:00 UTC 2022

Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy.

ACS applied materials & interfaces

Zhu J,Guo M,Cui Y,Meng Y,Ding J,Zeng W,Zhou W

Wed Feb 02 00:00:00 UTC 2022

Effect of B7-H4 downregulation induced by Toxoplasma gondii infection on dysfunction of decidual macrophages contributes to adverse pregnancy outcomes.

Parasites & vectors

Cui L,Wang Y,Ren L,Li Z,Jiang Y,Wang C,Liu X,Ren Y,Hu X

Tue Dec 13 00:00:00 UTC 2022

N-acetylcysteine attenuates atherosclerosis progression in aging LDL receptor deficient mice with preserved M2 macrophages and increased CD146.

Atherosclerosis

Zhu Q,Xiao Y,Jiang M,Liu X,Cui Y,Hao H,Flaker GC,Liu Q,Zhou S,Liu Z

Thu Sep 01 00:00:00 UTC 2022

TM9SF4 Is a Crucial Regulator of Inflammation and ER Stress in Inflammatory Bowel Disease.

Cellular and molecular gastroenterology and hepatology

Xie M,Mak JWY,Yu H,Cheng CTY,Chan HCH,Chan TT,Lau LHS,Wong MTL,Ko WH,Jiang L,Yao X

Mon Jul 25 00:00:00 UTC 2022

TRPV1 alleviates osteoarthritis by inhibiting M1 macrophage polarization via Ca²⁺/CaMKII/Nrf2 signaling pathway.

Cell death & disease

Lv Z,Xu X,Sun Z,Yang YX,Guo H,Li J,Sun K,Wu R,Xu J,Jiang Q,Ikegawa S,Shi D

Tue May 18 00:00:00 UTC 2021

Comprehensive analysis of prognostic immune-related genes and drug sensitivity in cervical cancer.

Cancer cell international

Pi YN,Guo JN,Lou G,Cui BB

Wed Dec 01 00:00:00 UTC 2021

Injectable shear-thinning polylysine hydrogels for localized immunotherapy of gastric cancer through repolarization of tumor-associated macrophages.

Biomaterials science

Yang Y,Yang Y,Chen M,Chen J,Wang J,Ma Y,Qian H

Tue Sep 28 00:00:00 UTC 2021

ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation.

Theranostics

Chen X,Gao A,Zhang F,Yang Z,Wang S,Fang Y,Li J,Wang J,Shi W,Wang L,Zheng Y,Sun Y

Fri Jul 23 00:00:00 UTC 2021

The Overexpression of CD80 and ISG15 Are Associated with the Progression and Metastasis of Breast Cancer by a Meta-Analysis Integrating Three Microarray Datasets.

Pathology oncology research : POR

Li Y,Bai W,Zhang L

Wed Jan 01 00:00:00 UTC 2020

The Overexpression of CD80 and ISG15 Are Associated with the Progression and Metastasis of Breast Cancer by a Meta-Analysis Integrating Three Microarray Datasets.

Pathology oncology research : POR

Li Y,Bai W,Zhang L

Wed Jan 01 00:00:00 UTC 2020

A significant role of transcription factors E2F in inflammation and tumorigenesis of nasopharyngeal carcinoma.

Biochemical and biophysical research communications

Liu P,Zhang X,Li Z,Wei L,Peng Q,Liu C,Wu Y,Yan Q,Ma J

Thu Apr 16 00:00:00 UTC 2020

Mutual antagonism between indoleamine 2,3-dioxygenase 1 and nuclear factor E2-related factor 2 regulates the maturation status of DCs in liver fibrosis.

Free radical biology & medicine

Mo C,Xie S,Zhong W,Zeng T,Huang S,Lai Y,Deng G,Zhou C,Yan W,Chen Y,Huang S,Gao L,Lv Z

Fri Nov 20 00:00:00 UTC 2020

CCL8 enhances sensitivity of cutaneous squamous cell carcinoma to photodynamic therapy by recruiting M1 macrophages.

Photodiagnosis and photodynamic therapy

Ji J,Wang P,Zhou Q,Zhu L,Zhang H,Zhang Y,Zheng Z,Bhatta AK,Zhang G,Wang X

Sat Jun 01 00:00:00 UTC 2019

CCL8 enhances sensitivity of cutaneous squamous cell carcinoma to photodynamic therapy by recruiting M1 macrophages.

Photodiagnosis and photodynamic therapy

Ji J,Wang P,Zhou Q,Zhu L,Zhang H,Zhang Y,Zheng Z,Bhatta AK,Zhang G,Wang X

Sat Jun 01 00:00:00 UTC 2019

Knockdown of LncRNA MALAT1 contributes to cell apoptosis via regulating NF-κB/CD80 axis in neonatal respiratory distress syndrome.

The international journal of biochemistry & cell biology

Juan C,Wang Q,Mao Y,Cao Q,Li S,Qiao C,Zhang D,Zhou G

Thu Nov 01 00:00:00 UTC 2018

Knockdown of LncRNA MALAT1 contributes to cell apoptosis via regulating NF-κB/CD80 axis in neonatal respiratory distress syndrome.

The international journal of biochemistry & cell biology

Juan C,Wang Q,Mao Y,Cao Q,Li S,Qiao C,Zhang D,Zhou G

Thu Nov 01 00:00:00 UTC 2018

The LncRNA MALAT1 regulates CD80 transcription via the NF-κB signaling pathway in the A549 cell line.

Biochemical and biophysical research communications

Juan C,Mao Y,Wang Q,Cao Q,Chen Y,Zhou G

Mon Sep 10 00:00:00 UTC 2018

The LncRNA MALAT1 regulates CD80 transcription via the NF-κB signaling pathway in the A549 cell line.

Biochemical and biophysical research communications

Juan C,Mao Y,Wang Q,Cao Q,Chen Y,Zhou G

Mon Sep 10 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: Activation B7-1 antigen; B-lymphocyte activation antigen B7; B7; B7 protein; BB1; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; sCD 80; sCD80; soluble CD 80; soluble CD80; T-lymphocyte activation antigen CD80; TS/A-1

View more View less

Gene Aliases: B7; B7-1; B7.1; B71; BB1; Cd28l; CD28LG; CD28LG1; CD80; LAB7; Ly-53; Ly53; MIC17; TSA1

View more View less

UniProt ID: (Human) P33681, (Mouse) Q00609

View more View less

Entrez Gene ID: (Human) 941, (Pig) 397161, (Mouse) 12519

View more View less

Function(s)

virus receptor activity protein binding coreceptor activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD80/B7-1 Monoclonal Antibody (1E2F10)

CD80/B7-1 Antibody (66406-1-IG) in IHC (P)

Product Details

66406-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!