Proteintech

CD81 Polyclonal Antibody

FIGURE: 1 / 1

CD81 Antibody (27855-1-AP) in WB

Jurkat cells and Raji cells were subjected to SDS PAGE followed by western blot with 27855-1-AP (CD81 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Product Details

27855-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 29 publications 29 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CD81 Fusion Protein Ag27298

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

650 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Differential Proteomic Profiles of Coronary Serum Exosomes in Acute Myocardial Infarction Patients with or Without Diabetes Mellitus: ANGPTL6 Accelerates Regeneration of Endothelial Cells Treated with Rapamycin via MAPK Pathways.

Cardiovascular drugs and therapy

Wang W,Zhao Y,Zhu P,Jia X,Wang C,Zhang Q,Li H,Wang J,Hou Y

Thu Feb 01 00:00:00 UTC 2024

Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1-to-M2 transformation.

Frontiers in physiology

Zhang C,Liao W,Li W,Li M,Xu X,Sun H,Xue Y,Liu L,Qiu J,Zhang C,Zhang X,Ye J,Du J,Deng DYB,Deng W,Li T

Tue Mar 07 00:00:00 UTC 2023

A 3D culture system improves the yield of MSCs-derived extracellular vesicles and enhances their therapeutic efficacy for heart repair.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Sun L,Ji Y,Chi B,Xiao T,Li C,Yan X,Xiong X,Mao L,Cai D,Zou A,Wang Y,Zhang L,Tang L,Wang Q

Mon May 01 00:00:00 UTC 2023

Malaria-derived exosomes exacerbate liver injury during blood stage of Plasmodium berghei infection.

Acta tropica

Zhang X,Zhang M,Wang QR,Hou X,Zhou T,Liu J,Wang Q,Liu W,Liu X,Jin X,Liu Z,Huang B

Wed Mar 01 00:00:00 UTC 2023

Extracellular vesicle-transmitted miR-671-5p alleviates lung inflammation and injury by regulating the AAK1/NF-κB axis.

Molecular therapy : the journal of the American Society of Gene Therapy

Lian J,Zhu X,Du J,Huang B,Zhao F,Ma C,Guo R,Zhang Y,Ji L,Yahaya BH,Lin J

Wed May 03 00:00:00 UTC 2023

Exosomes Derived from AT2R-Overexpressing BMSC Prevent Restenosis After Carotid Artery Injury by Attenuating the Injury-Induced Neointimal Hyperplasia.

Journal of cardiovascular translational research

Zou X,Liao Y,Liu Z,Xu X,Sun W,Qin H,Wang H,Liu J,Jing T

Wed Feb 01 00:00:00 UTC 2023

Low-intensity pulsed ultrasound (LIPUS) enhances the anti-inflammatory effects of bone marrow mesenchymal stem cells (BMSCs)-derived extracellular vesicles.

Cellular & molecular biology letters

Li X,Zhong Y,Zhou W,Song Y,Li W,Jin Q,Gao T,Zhang L,Xie M

Mon Jan 30 00:00:00 UTC 2023

Inflammatory capacity of exosomes released in the early stages of acute pancreatitis predicts the severity of the disease.

The Journal of pathology

Carrascal M,Areny-Balagueró A,de-Madaria E,Cárdenas-Jaén K,García-Rayado G,Rivera R,Martin Mateos RM,Pascual-Moreno I,Gironella M,Abian J,Closa D

Sat Jan 01 00:00:00 UTC 2022

Melanoma stem cells promote metastasis via exosomal miR-1268a inactivation of autophagy.

Biological research

Li X,Liu D,Chen H,Zeng B,Zhao Q,Zhang Y,Chen Y,Wang J,Xing HR

Sat Oct 01 00:00:00 UTC 2022

Correlation of alpha-1 antitrypsin levels and exosome associated neutrophil elastase endothelial injury in subjects with SARS-CoV2 infection.

PloS one

Lascano J,Oshins R,Eagan C,Wadood Z,Qiang X,Flagg T,Scindia Y,Mehrad B,Brantly M,Khodayari N

Tue Sep 13 00:00:00 UTC 2022

Cancer-associated fibroblasts promote the stemness and progression of renal cell carcinoma via exosomal miR-181d-5p.

Cell death discovery

Ding M,Zhao X,Chen X,Diao W,Kan Y,Cao W,Chen W,Jiang B,Qin H,Gao J,Zhuang J,Zhang Q,Guo H

Tue Nov 01 00:00:00 UTC 2022

Cancer-associated fibroblasts promote the stemness and progression of renal cell carcinoma via exosomal miR-181d-5p.

Cell death discovery

Ding M,Zhao X,Chen X,Diao W,Kan Y,Cao W,Chen W,Jiang B,Qin H,Gao J,Zhuang J,Zhang Q,Guo H

Tue Nov 01 00:00:00 UTC 2022

Cancer-associated fibroblasts promote the stemness and progression of renal cell carcinoma via exosomal miR-181d-5p.

Cell death discovery

Ding M,Zhao X,Chen X,Diao W,Kan Y,Cao W,Chen W,Jiang B,Qin H,Gao J,Zhuang J,Zhang Q,Guo H

Tue Nov 01 00:00:00 UTC 2022

Cancer-associated fibroblasts promote the stemness and progression of renal cell carcinoma via exosomal miR-181d-5p.

Cell death discovery

Ding M,Zhao X,Chen X,Diao W,Kan Y,Cao W,Chen W,Jiang B,Qin H,Gao J,Zhuang J,Zhang Q,Guo H

Tue Nov 01 00:00:00 UTC 2022

Proteomic profiling of plasma exosomes reveals CD82 involvement in the development of esophageal squamous cell carcinoma.

Journal of proteomics

Yang L,Salai A,Sun X,Liu Q,Liu T,Zhang Q,Tuerxun A,Tan Y,Zheng S,Lu X

Mon Aug 15 00:00:00 UTC 2022

HucMSC-Ex carrying miR-203a-3p.2 ameliorates colitis through the suppression of caspase11/4-induced macrophage pyroptosis.

International immunopharmacology

Xu Y,Tang X,Fang A,Yan J,Kofi Wiredu Ocansey D,Zhang X,Mao F

Thu Sep 01 00:00:00 UTC 2022

Engineered exosome as targeted lncRNA MEG3 delivery vehicles for osteosarcoma therapy.

Journal of controlled release : official journal of the Controlled Release Society

Huang X,Wu W,Jing D,Yang L,Guo H,Wang L,Zhang W,Pu F,Shao Z

Tue Mar 01 00:00:00 UTC 2022

iRGD-modified exosomes-delivered BCL6 siRNA inhibit the progression of diffuse large B-cell lymphoma.

Frontiers in oncology

Liu Q,Dai G,Wu Y,Zhang M,Yang M,Wang X,Song M,Li X,Xia R,Wu Z

Sat Aug 20 00:00:00 UTC 2022

MiR-199a-3p in mouse bone marrow mesenchymal stem cell exosomes increases epithelial sodium channel expression in lung injury.

Fundamental & clinical pharmacology

Chen L,Hou Y,Du D,Cui Y,Nie H,Ding Y

Thu Dec 01 00:00:00 UTC 2022

Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.

Translational oncology

Liu X,Li N,Zhang C,Wu X,Zhang S,Dong G,Liu G

Sun May 01 00:00:00 UTC 2022

Exosomes derived from human umbilical cord mesenchymal stem cells regulate lymphangiogenesis via the miR-302d-3p/VEGFR3/AKT axis to ameliorate inflammatory bowel disease.

International immunopharmacology

Zhang L,Yuan J,Kofi Wiredu Ocansey D,Lu B,Wan A,Chen X,Zhang X,Qiu W,Mao F

Thu Sep 01 00:00:00 UTC 2022

Exosome-transmitted miR-3124-5p promotes cholangiocarcinoma development via targeting GDF11.

Frontiers in oncology

Gao H,He Z,Gao C,Liu N,Zhang Z,Niu W,Niu J,Peng C

Fri Aug 19 00:00:00 UTC 2022

The effect of lncRNA MIR155HG-modified MSCs and exosome delivery to synergistically attenuate vein graft intimal hyperplasia.

Stem cell research & therapy

Bai X,Qi Z,Zhu M,Lu Z,Zhao X,Zhang L,Song G

Fri Nov 04 00:00:00 UTC 2022

TGF-β1-mediated exosomal lnc-MMP2-2 increases blood-brain barrier permeability via the miRNA-1207-5p/EPB41L5 axis to promote non-small cell lung cancer brain metastasis.

Cell death & disease

Wu D,Deng S,Li L,Liu T,Zhang T,Li J,Yu Y,Xu Y

Tue Jul 20 00:00:00 UTC 2021

Exosomes secreted from mesenchymal stem cells mediate the regeneration of endothelial cells treated with rapamycin by delivering pro-angiogenic microRNAs.

Experimental cell research

Wang W,Zhao Y,Li H,Zhang Y,Jia X,Wang C,Zhu P,Wang J,Hou Y

Mon Feb 01 00:00:00 UTC 2021

Exosomal lncRNA LINC01711 facilitates metastasis of esophageal squamous cell carcinoma via the miR-326/FSCN1 axis.

Aging

Xu ML,Liu TC,Dong FX,Meng LX,Ling AX,Liu S

Mon Aug 09 00:00:00 UTC 2021

Plasma‑derived exosomal pyruvate kinase isoenzyme type M2 accelerates the proliferation and motility of oesophageal squamous cell carcinoma cells.

Oncology reports

Yang L,Zheng S,Liu Q,Liu T,Zhang Q,Han X,Tuerxun A,Lu X

Fri Oct 01 00:00:00 UTC 2021

KLF4-mediated upregulation of CD9 and CD81 suppresses hepatocellular carcinoma development via JNK signaling.

Cell death & disease

Li Y,Yu S,Li L,Chen J,Quan M,Li Q,Gao Y

Wed Apr 29 00:00:00 UTC 2020

KLF4-mediated upregulation of CD9 and CD81 suppresses hepatocellular carcinoma development via JNK signaling.

Cell death & disease

Li Y,Yu S,Li L,Chen J,Quan M,Li Q,Gao Y

Wed Apr 29 00:00:00 UTC 2020

Insight into the proteomic profiling of exosomes secreted by human OM-MSCs reveals a new potential therapy.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Xun C,Ge L,Tang F,Wang L,Zhuo Y,Long L,Qi J,Hu L,Duan D,Chen P,Lu M

Sun Nov 01 00:00:00 UTC 2020

Stem cells international

Chen S,Wang Y,Liao L,Meng L,Li J,Shi C,Han H,Zheng X,Shen H

Tue Apr 19 00:00:00 UTC 2022

Human umbilical cord mesenchymal stem cell-derived exosomes act via the miR-1263/Mob1/Hippo signaling pathway to prevent apoptosis in disuse osteoporosis.

Biochemical and biophysical research communications

Yang BC,Kuang MJ,Kang JY,Zhao J,Ma JX,Ma XL

Thu Apr 16 00:00:00 UTC 2020

Exosomes derived from Wharton's jelly of human umbilical cord mesenchymal stem cells reduce osteocyte apoptosis in glucocorticoid-induced osteonecrosis of the femoral head in rats via the miR-21-PTEN-AKT signalling pathway.

International journal of biological sciences

Kuang MJ,Huang Y,Zhao XG,Zhang R,Ma JX,Wang DC,Ma XL

Mon Apr 13 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: 26 kDa cell surface protein TAPA-1; CD81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); Target of the antiproliferative antibody 1; Tetraspanin-28; Tetraspanin28; Tspan-28

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Gene Aliases: CD81; CVID6; S5.7; TAPA1; TSPAN28

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UniProt ID: (Human) P60033

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Entrez Gene ID: (Human) 975

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Function(s)

protein binding MHC class II protein complex binding transferrin receptor binding

Process(es)

activation of MAPK activity cell surface receptor signaling pathway protein localization cell proliferation positive regulation of cell proliferation response to wounding positive regulation of B cell proliferation receptor internalization regulation of protein stability regulation of growth positive regulation of 1-phosphatidylinositol 4-kinase activity positive regulation of transcription from RNA polymerase II promoter viral entry into host cell receptor-mediated virion attachment to host cell positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response protein localization to lysosome cellular response to low-density lipoprotein particle stimulus positive regulation of protein catabolic process in the vacuole regulation of cell motility

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD81 Polyclonal Antibody

CD81 Antibody (27855-1-AP) in WB

Product Details

27855-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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