Invitrogen

CD83 Monoclonal Antibody (HB15e), FITC, eBioscience™

4 Published Figures

12 References

View all (49) CD83 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

FIGURE: 1 / 5

CD83 Antibody (11-0839-42) in Flow

Staining of 1 day PHA-stimulated human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD83 FITC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

11-0839-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 12 publications 12 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HB15e

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1633400

Product Specific Information

Description: The HB15e monoclonal antibody reacts with human CD83, a 45 kDa transmembrane glycoprotein. CD83, a member of the Ig superfamily, is expressed on cultured dendritic cells, interdigitating, follicular, and circulating dendritic cells as well as some proliferating lymphocytes, and human cell lines express this antigen. While the function of CD83 is unclear, it can serve as a useful marker for mature human blood dendritic cells.

Applications Reported: The HB15e antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HB15e antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD83 cell surface antigen is a 40-45kD glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA. In immunohistology, CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres. CD83 is also expressed by Langerhan's cells in the skin. The CD83 antigen is a 186-amino-acid single-chain glycoprotein and this molecule is a member of the immunoglobulin superfamily that is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail. CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein. However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage. CD83+ cells coexpress the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules. Diseases associated with CD83 dysfunction include plague and Rift Valley Fever.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Cathepsin S inhibition in dendritic cells prevents Th17 cell differentiation in perivascular adipose tissues following vascular injury in diabetic rats.

Journal of biochemical and molecular toxicology

Peng H,Lv Y,Li C,Cheng Z,He S,Wang C,Liu J

11-0839-42 was used in Flow Cytometry to investigate the role of CTSS in restenosis following carotid injury in diabetic rats.

Fri Sep 01 00:00:00 UTC 2023

STAT3 phosphorylation at Ser727 and Tyr705 differentially regulates the EMT-MET switch and cancer metastasis.

Oncogene

Lin WH,Chang YW,Hong MX,Hsu TC,Lee KC,Lin C,Lee JL

Published figure using CD83 monoclonal antibody (Product # 11-0839-42) in Flow Cytometry

Fri Jan 01 00:00:00 UTC 2021

Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival.

Protein & cell

Tong D,Zhang L,Ning F,Xu Y,Hu X,Shi Y

Published figure using CD83 monoclonal antibody (Product # 11-0839-42) in Flow Cytometry

Sat Feb 01 00:00:00 UTC 2020

Herpes Simplex Virus Type-2 Paralyzes the Function of Monocyte-Derived Dendritic Cells.

Viruses

Grosche L,Mühl-Zürbes P,Ciblis B,Krawczyk A,Kuhnt C,Kamm L,Steinkasserer A,Heilingloh CS

Published figure using CD83 monoclonal antibody (Product # 11-0839-42) in Flow Cytometry

Thu Jan 16 00:00:00 UTC 2020

TNF blockade induces a dysregulated type I interferon response without autoimmunity in paradoxical psoriasis.

Nature communications

Conrad C,Di Domizio J,Mylonas A,Belkhodja C,Demaria O,Navarini AA,Lapointe AK,French LE,Vernez M,Gilliet M

11-0839-42 was used in Flow Cytometry to study the pathogenesis of paradoxical psoriasis and its distinction from classical psoriasis.

Tue Jan 02 00:00:00 UTC 2018

Dexamethasone and Monophosphoryl Lipid A Induce a Distinctive Profile on Monocyte-Derived Dendritic Cells through Transcriptional Modulation of Genes Associated With Essential Processes of the Immune Response.

Frontiers in immunology

García-González PA,Schinnerling K,Sepúlveda-Gutiérrez A,Maggi J,Mehdi AM,Nel HJ,Pesce B,Larrondo ML,Aravena O,Molina MC,Catalán D,Thomas R,Verdugo RA,Aguillón JC

11-0839 was used in Flow cytometry/Cell sorting to study the transcriptional profile of monocyte-derived dendritic cells from healthy subjects to identify molecular regulators and pathways associated with the induction of tolerogenic properties in tolerogenic dendritic cells.

Wed Nov 20 00:00:00 UTC 2019

Hepatitis B Virus Surface Antigen Activates Myeloid Dendritic Cells via a Soluble CD14-Dependent Mechanism.

Journal of virology

van Montfoort N,van der Aa E,van den Bosch A,Brouwers H,Vanwolleghem T,Janssen HLA,Javanbakht H,Buschow SI,Woltman AM

11-0839 was used in Flow cytometry/Cell sorting to identify CD14 and TLR4 as receptors for HBsAg, the main HBV envelope antigen.

Fri Jul 15 00:00:00 UTC 2016

Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen.

Infection and immunity

Miles B,Zakhary I,El-Awady A,Scisci E,Carrion J,O'Neill JC,Rawlings A,Stern JK,Susin C,Cutler CW

11-0839 was used in Immunofluorescence to investigate how the alteration in chemokine-chemokine receptor expression contributes to pathogen trafficking via myeloid dendritic cells, showing that P.gingivalis infection induces an angiogenic migratory profile and disrupts secondary lymphoid organ-homing CCR7.

Wed Jan 01 00:00:00 UTC 2014

Novel immunomodulators from hard ticks selectively reprogramme human dendritic cell responses.

PLoS pathogens

Preston SG,Majtán J,Kouremenou C,Rysnik O,Burger LF,Cabezas Cruz A,Chiong Guzman M,Nunn MA,Paesen GC,Nuttall PA,Austyn JM

11-0839 was used in Flow cytometry/Cell sorting to provide a previously unknown mechanism for parasite-induced subversion of adaptive immunity.

Fri Jan 17 00:00:00 UTC 2014

Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen.

Journal of leukocyte biology

Miles B,Scisci E,Carrion J,Sabino GJ,Genco CA,Cutler CW

11-0839 was used in Immunofluorescence to provide insights into how P. gingivalis may influence immune homeostasis and promote chronic inflammation.

Thu Aug 01 00:00:00 UTC 2013

Early secreted antigenic target of 6-kDa protein of Mycobacterium tuberculosis primes dendritic cells to stimulate Th17 and inhibit Th1 immune responses.

Journal of immunology (Baltimore, Md. : 1950)

Wang X,Barnes PF,Huang F,Alvarez IB,Neuenschwander PF,Sherman DR,Samten B

11-0839 was used in Flow cytometry/Cell sorting to study the effect of early secreted antigenic target of 6 kDa (ESAT-6) on human dendritic cells.

Sat Sep 15 00:00:00 UTC 2012

Dendritic cells derived from hemozoin-loaded monocytes display a partial maturation phenotype that promotes HIV-1 trans-infection of CD4+ T cells and virus replication.

Journal of immunology (Baltimore, Md. : 1950)

Diou J,Tardif MR,Barat C,Tremblay MJ

11-0839 was used in Flow cytometry/Cell sorting to investigate the effect of malarial pigment hemozoin on HIV-1 transmission, showing that hemozoid-loaded monocytes promote HIV-1 trans-infection and replication.

Mon Mar 15 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: B-cell activation protein; CD antigen CD83; CD83; CD83 antigen; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); Cell surface protein HB15; cell-surface glycoprotein; hCD83

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Gene Aliases: BL11; CD83; HB15

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UniProt ID: (Human) Q01151

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Entrez Gene ID: (Human) 9308

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Process(es)

regulation of cytokine production defense response humoral immune response signal transduction response to organic cyclic compound negative regulation of interleukin-4 production positive regulation of interleukin-10 production positive regulation of interleukin-2 production positive regulation of CD4-positive, alpha-beta T cell differentiation

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CD83 Monoclonal Antibody (HB15e), FITC, eBioscience™

CD83 Antibody (11-0839-42) in Flow

Product Details

11-0839-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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