Invitrogen

CD83 Monoclonal Antibody (Michel-17 (Michel17)), PE, eBioscience™

4 Published Figures

16 References

View all (49) CD83 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

FIGURE: 1 / 5

CD83 Antibody (12-0831-82) in Flow

Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 µg of Rat IgG1 K Isotype Contol PE (Product # 12-4301-82) (blue histogram) or 0.25 µg of Anti-Mouse CD83 PE (purple histogram). Total viable cells were used for analysis.

Product Image Gallery

Product Details

12-0831-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 15 publications 15 publications

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Michel-17 (Michel17)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_465758

Product Specific Information

Description: The Michel-17 monoclonal antibody reacts with mouse CD83, a 45kDa cell surface glycoprotein and a member of the Ig superfamily. The mouse CD83 antigen is expressed predominantly on mature DC and activated lymphocytes. Cross-linking of CD83 with Michel-17 on DC or activated T cells does not induce any activation signal. CD83 plays an important role in T cell development through interaction with its ligand. CD83-Ig protein has revealed the presence of a CD83 ligand expressed mainly by B220^+ cells in mouse spleen.

Applications Reported: The Michel-17 (Michel17) antibody has been reported for use in flow cytometric analysis.

Applications Tested: The Michel-17 (Michel17) antibody has been tested by flow cytometric analysis of LPS activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD83 cell surface antigen is a 40-45kD glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA. In immunohistology, CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres. CD83 is also expressed by Langerhan's cells in the skin. The CD83 antigen is a 186-amino-acid single-chain glycoprotein and this molecule is a member of the immunoglobulin superfamily that is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail. CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein. However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage. CD83+ cells coexpress the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules. Diseases associated with CD83 dysfunction include plague and Rift Valley Fever.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Effect of mesenchymal stem cell-derived exosomes on the induction of mouse tolerogenic dendritic cells.

Journal of cellular physiology

Shahir M,Mahmoud Hashemi S,Asadirad A,Varahram M,Kazempour-Dizaji M,Folkerts G,Garssen J,Adcock I,Mortaz E

12-0831 was used in Flow cytometry/Cell sorting to investigate the effects of mesenchymal stem cell-derived exosomes and to understand their role in modulating the mouse tolergenic dendritic cell immune responses.

Thu Oct 01 00:00:00 UTC 2020

Immunoadjuvant Activity of Fucoidans from the Brown Alga Fucus evanescens.

Marine drugs

Kuznetsova TA,Smolina TP,Makarenkova ID,Ivanushko LA,Persiyanova EV,Ermakova SP,Silchenko AS,Zaporozhets TS,Besednova NN,Fedyanina LN,Kryzhanovsky SP

12-0831 was used in Flow cytometry/Cell sorting to comparatively evaluate the effect of fucoidans from the brown alga Fucus evanescens on effector functions of innate and adaptive immunity cells in vitro and in vivo.

Wed Mar 11 00:00:00 UTC 2020

Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome.

Nature communications

Komban RJ,Strömberg A,Biram A,Cervin J,Lebrero-Fernández C,Mabbott N,Yrlid U,Shulman Z,Bemark M,Lycke N

12-0831-82 was used in Flow Cytometry to report a M cell-B cell antigen-specific transporting pathway in PP that is independent of DC. We propose that this antigen transporting pathway has a critical role in gut IgA responses, and should be taken into account when developing mucosal vaccines.

Mon Jun 03 00:00:00 UTC 2019

Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome.

Nature communications

Komban RJ,Strömberg A,Biram A,Cervin J,Lebrero-Fernández C,Mabbott N,Yrlid U,Shulman Z,Bemark M,Lycke N

Mon Jun 03 00:00:00 UTC 2019

Der f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells.

Scientific reports

Wang H,Lin J,Zeng L,Ouyang C,Ran P,Yang P,Liu Z

Thu Aug 17 00:00:00 UTC 2017

Pplase of Dermatophagoides farinae promotes ovalbumin-induced airway allergy by modulating the functions of dendritic cells in a mouse model.

Scientific reports

Wang H,Mo L,Xiao X,An S,Liu X,Ba J,Wu W,Ran P,Yang P,Liu Z

12-0831 was used in an ELISA assay to demonstrate that Pplase is not a dustmite allergen but can enhance allergic responses.

Mon Feb 27 00:00:00 UTC 2017

Pplase of Dermatophagoides farinae promotes ovalbumin-induced airway allergy by modulating the functions of dendritic cells in a mouse model.

Scientific reports

Wang H,Mo L,Xiao X,An S,Liu X,Ba J,Wu W,Ran P,Yang P,Liu Z

12-0831 was used in an ELISA assay to demonstrate that Pplase is not a dustmite allergen but can enhance allergic responses.

Mon Feb 27 00:00:00 UTC 2017

Thiol peroxiredoxin, a novel allergen from Bombyx mori, modulates functions of macrophages and dendritic cells.

American journal of translational research

Wang H,Hu W,Liang Z,Zeng L,Li J,Yan H,Yang P,Liu Z,Wang L

12-0831 was used in Flow cytometry/Cell sorting to study how thiol peroxiredoxin, a novel allergen from Bombyx mori, modulates functions of macrophages and dendritic cells.

Thu Oct 01 00:00:00 UTC 2020

Enhanced Anti-Tumor Efficacy through a Combination of Integrin αvβ6-Targeted Photodynamic Therapy and Immune Checkpoint Inhibition.

Theranostics

Gao L,Zhang C,Gao D,Liu H,Yu X,Lai J,Wang F,Lin J,Liu Z

12-0831 was used in Flow cytometry/Cell sorting to investigate whether integrin αvβ6-targeted photodynamic therapy (PDT) of tumors using a DSAB-HK could trigger the host immune response, showing that DSAB-HK led to inhibited growth of 4T1 tumours and promoted maturation of dendritic cells.

Tue Dec 13 00:00:00 UTC 2016

Angiotensin converting enzyme inhibitors and angiotensin II receptor antagonist attenuate tumor growth via polarization of neutrophils toward an antitumor phenotype.

Oncoimmunology

Shrestha S,Noh JM,Kim SY,Ham HY,Kim YJ,Yun YJ,Kim MJ,Kwon MS,Song DK,Hong CW

12-0831 was used in Flow cytometry/Cell sorting to investigate how modulation of Ang II pathway attenuates tumour growth via polarisation of neutrophils to an antitumoural phenotype.

Tue Jan 12 00:00:00 UTC 2021

Distinct roles for MyD88 and Toll-like receptor 2 during Leishmania braziliensis infection in mice.

Infection and immunity

Vargas-Inchaustegui DA,Tai W,Xin L,Hogg AE,Corry DB,Soong L

12-0831 was used in Flow cytometry/Cell sorting to investigate whether TLRs are involved in L. braziliensis-mediated dendritic cell activation, showing distinct roles for MyD88 and TLR2 in infection.

Wed Jul 01 00:00:00 UTC 2009

Nitric oxide is a key component in inflammation-accelerated tumorigenesis.

Cancer research

Hussain SP,He P,Subleski J,Hofseth LJ,Trivers GE,Mechanic L,Hofseth AB,Bernard M,Schwank J,Nguyen G,Mathe E,Djurickovic D,Haines D,Weiss J,Back T,Gruys E,Laubach VE,Wiltrout RH,Harris CC

12-0831 was used in Flow cytometry/Cell sorting to test the hypothesis that an inflammatory microenvironment with an enhanced level of nitric oxide accelerates spontaneous tumour development (tumorigenesis).

Mon Sep 01 00:00:00 UTC 2008

Leishmania braziliensis infection induces dendritic cell activation, ISG15 transcription, and the generation of protective immune responses.

Journal of immunology (Baltimore, Md. : 1950)

Vargas-Inchaustegui DA,Xin L,Soong L

12-0831 was used in Flow cytometry/Cell sorting to investigate whether Leishmania braziliensis infection efficiently triggers innate responses in dendritic cells and the subsequent adaptive immune responses for parasite clearance.

Sun Jun 01 00:00:00 UTC 2008

Down-regulation of dendritic cell signaling pathways by Leishmania amazonensis amastigotes.

Molecular immunology

Xin L,Li K,Soong L

12-0831 was used in Flow cytometry/Cell sorting to suggest that La parasites, especially in their intracellular forms, have evolved unique strategies to actively down-regulate early innate signalling events.

Tue Jul 01 00:00:00 UTC 2008

Effects of CXCL10 on dendritic cell and CD4+ T-cell functions during Leishmania amazonensis infection.

Infection and immunity

Vasquez RE,Xin L,Soong L

12-0831 was used in Flow cytometry/Cell sorting to investigate the mechanism underlying enhanced immunity against L. amazonensis infection following CXC10 treatment, showing that treatment has an effect on dendritic cell and CD4+ T-cell functions.

Tue Jan 01 00:00:00 UTC 2008

Role of natural killer cells in modulating dendritic cell responses to Leishmania amazonensis infection.

Infection and immunity

Sanabria MX,Vargas-Inchaustegui DA,Xin L,Soong L

12-0831 was used in Flow cytometry/Cell sorting to suggest differential roles of DC-NK cell cross talk at different stages of Leishmania infection.

Sat Nov 01 00:00:00 UTC 2008

Role of interleukin-1beta in activating the CD11c(high) CD45RB- dendritic cell subset and priming Leishmania amazonensis-specific CD4+ T cells in vitro and in vivo.

Infection and immunity

Xin L,Li Y,Soong L

12-0831 was used in Flow cytometry/Cell sorting to investigate the mechanism by which the Leishmania amazonensis parasite modulates antigen-presenting cells and disrupts the immune system.

Mon Oct 01 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: CD antigen CD83; CD83; CD83 antigen; mCD83

View more View less

Gene Aliases: Cd83

View more View less

UniProt ID: (Mouse) O88324

View more View less

Entrez Gene ID: (Mouse) 12522

View more View less

Process(es)

response to organic cyclic compound negative regulation of interleukin-4 production positive regulation of interleukin-10 production positive regulation of interleukin-2 production positive regulation of CD4-positive, alpha-beta T cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD83 Monoclonal Antibody (Michel-17 (Michel17)), PE, eBioscience™

CD83 Antibody (12-0831-82) in Flow

Product Details

12-0831-82

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!