Invitrogen

CD85d (ILT4) Monoclonal Antibody (27D6), FITC, eBioscience™

CD85d (ILT4) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

CD85d (ILT4) Antibody (11-5148-42) in Flow

Staining of normal human peripheral blood cells with Rat IgM kappa Isotype Control FITC (Product # 11-4341-82) (open histogram) or Anti-Human CD85d (ILT4) FITC (filled histogram). Cells in the monocyte gate were used for analysis.

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Product Details

11-5148-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgM

Recommended Isotype Control

Rat IgM Isotype Control (eBRM), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

27D6

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10853660

Product Specific Information

Description: The monoclonal antibody 27D6 recognizes the CD85d protein also known as ILT4, LIR-2. It is a member of the ILT (immunoglobulin-like transcript)/ LIR (leuokocyte Ig-like receptor) MIR (monocytes Ig-like receptor) family. CD85d is a 110 kDa single transmembrane receptor containing 3 Ig-like domains and a long cytoplasmic domain containing ITIMs (immunoreceptor tyrosine-based inhibitory motifs). Expression is confined to monocytes, macrophages and dendritic cells. The ligand for CD85d is non-classical class I HLA-G, -B and G1. Upon ligand binding, tyrosine phosphatase SHP-1 interacts with the ITIM. Interaction of HLA-G with ILT4/CD85d is thought to contribute to tolerance during fetal implantation. Additionally it is the levels of ILT3 and ILT4 on DCs that suggests a role in organ allograft rejection.

The monoclonal antibody 27D6 has been shown to block binding of ligand (HLA-G tetramer).

Applications Reported: This 27D6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 27D6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD85d, also known as LILRB2 or ILT4, is a member of the immunoglobulin-like transcript (ILT), leukocyte Ig-like receptor (LIR), and monocyte Ig-like receptor (MIR) family. It is a 110 kDa single transmembrane receptor with three Ig-like domains and a long cytoplasmic domain containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs). CD85d is expressed on monocytes, macrophages, and dendritic cells. The receptor binds to non-classical class I molecules such as HLA-G, -B, and G1. Upon ligand binding, the tyrosine phosphatase SHP-1 interacts with the ITIMs, transducing a negative signal that inhibits immune response stimulation. This interaction is thought to contribute to tolerance during fetal implantation and plays a role in controlling inflammatory responses and cytotoxicity, helping to focus the immune response and limit autoreactivity. CD85d is also involved in organ allograft rejection, with the levels of ILT3 and ILT4 on dendritic cells suggesting its role in this process. The LILRB2 gene, located in a gene cluster at chromosomal region 19q13.4, encodes multiple transcript variants with different isoforms, highlighting its complexity and importance in immune regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Role of Nuclear Factor (Erythroid-Derived 2)-Like 2 Signaling for Effects of Fumaric Acid Esters on Dendritic Cells.

Frontiers in immunology

Hammer A,Waschbisch A,Knippertz I,Zinser E,Berg J,Jörg S,Kuhbandner K,David C,Pi J,Bayas A,Lee DH,Haghikia A,Gold R,Steinkasserer A,Linker RA

Wed Nov 20 00:00:00 UTC 2019

NOD1 modulates IL-10 signalling in human dendritic cells.

Scientific reports

Neuper T,Ellwanger K,Schwarz H,Kufer TA,Duschl A,Horejs-Hoeck J

11-5148 was used in Flow cytometry/Cell sorting to investigate the regulatory role that NOD1 plays by modulating IL-10 signalling in human dendritic cells during anti-inflammatory immune responses.

Fri Apr 21 00:00:00 UTC 2017

A Phenotypic Analysis of Regulatory T Cells and Uterine NK Cells from First Trimester Pregnancies and Associations with HLA-G.

American journal of reproductive immunology (New York, N.Y. : 1989)

Djurisic S,Skibsted L,Hviid TV

11-5148-42 was used in Flow cytometry/Cell sorting to conclude that the NK subset in the placental bed displays a unique phenotype that may be influenced by mHLA-G on trophoblast cells and locally accumulated sHLA-G in the uterus.

Sun Nov 01 00:00:00 UTC 2015

Transfer of regulatory properties from tolerogenic to proinflammatory dendritic cells via induced autoreactive regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Kleijwegt FS,Laban S,Duinkerken G,Joosten AM,Koeleman BP,Nikolic T,Roep BO

11-5148 was used in Flow cytometry/Cell sorting to study the mechanism by which infectious tolerance applies to human dendritic cells.

Thu Dec 15 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: CD85 antigen-like family member D; CD85d; CD85d antigen; Ig-like transcript 4; ILT-4; Immunoglobulin-like transcript 4; leucocyte Ig-like receptor B2; Leukocyte immunoglobulin-like receptor subfamily B member 2; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2; LIR-2; LIR2CD85D; MIR-10; MIR10LILRA6; Monocyte/macrophage immunoglobulin-like receptor 10; myeloid inhibitory receptor 10

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Gene Aliases: CD85D; ILT-4; ILT4; LILRB2; LIR-2; LIR2; MIR-10; MIR10

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UniProt ID: (Human) Q8N423

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Entrez Gene ID: (Human) 10288

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Function(s)

receptor activity protein binding protein phosphatase 1 binding MHC class Ib protein binding inhibitory MHC class I receptor activity MHC class I protein binding cell adhesion molecule binding

Process(es)

adaptive immune response negative regulation of antigen processing and presentation positive regulation of tolerance induction positive regulation of T cell tolerance induction immune response-inhibiting cell surface receptor signaling pathway Fc receptor mediated inhibitory signaling pathway immune response cellular defense response signal transduction cell surface receptor signaling pathway cell-cell signaling positive regulation of interleukin-6 production heterotypic cell-cell adhesion positive regulation of T cell proliferation negative regulation of T cell proliferation positive regulation of regulatory T cell differentiation regulation of immune response negative regulation of immune response negative regulation of calcium ion transport cellular response to lipopolysaccharide regulation of dendritic cell differentiation

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CD85d (ILT4) Monoclonal Antibody (27D6), FITC, eBioscience™

CD85d (ILT4) Antibody (11-5148-42) in Flow

Product Details

11-5148-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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