Invitrogen

CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor™ Blue 660-120S, eBioscience™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

H043T03B08-A

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.6 µg)/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

HP-F1

Conjugate

NovaFluor™ Blue 660-120S NovaFluor™ Blue 660-120S NovaFluor™ Blue 660-120S

Excitation/Emission Max

492/665 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

RRID

AB_3098530 (AB_2925927)

Product Specific Information

Description: The monoclonal antibody HP-F1 recognizes CD85j, also known as ILT2, LILRB1, and LIR1. CD85j is a member of the ILT (immunoglobulin-like transcript)/LIR (leukocyte Ig-like receptor)/MIR (monocyte Ig-like receptor) family. CD85j is a single transmembrane glycoprotein with a long cytoplasmic domain containing 4 ITIMs which signal through interactions with SHP-1. Expression is found on myeloid cells (monocytes and dendritic cells) and some lymphoid cells including, subsets of NK, T and B cells. Expression has been correlated with leukemias such as ALL and CLL. Expression on CD8+ cells correlates with effector cell function and plays an important role in viral infections, including HIV, Ebstein Barr and CMV. The ligands for CD85j are MHC Class I molecules such as HLA-G, A, F, B27, E and F. The monoclonal antibody HP-F1 has been shown to reduce the amount of CD16- dependent cytolytic activity of functional NK cells.

Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .

Applications Reported: This HP-F1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HP-F1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.

NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more

Excitation: 509 nm; Emission: 665 nm; Laser: 488 nm (Blue) Laser

Target Information

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Bioinformatics

Protein Aliases: CD85 antigen-like family member J; CD85j; Ig-like transcript 2; ILT-2; Immunoglobulin-like transcript 2; leucocyte Ig-like receptor B1; Leukocyte immunoglobulin-like receptor subfamily B member 1; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1; LIR-1; MIR-7; Monocyte/macrophage immunoglobulin-like receptor 7; myeloid inhibitory receptor 7

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Gene Aliases: CD85J; ILT-2; ILT2; LILRB1; LIR-1; LIR1; MIR-7; MIR7; PIR-B; PIRB

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UniProt ID: (Human) Q8NHL6

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Entrez Gene ID: (Human) 10859

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Function(s)
protein phosphatase 1 binding HLA-A specific inhibitory MHC class I receptor activity HLA-B specific inhibitory MHC class I receptor activity MHC class I receptor activity SH2 domain binding MHC class I protein binding protein homodimerization activity
Process(es)
negative regulation of T cell mediated cytotoxicity positive regulation of defense response to virus by host adaptive immune response T cell proliferation involved in immune response negative regulation of cytokine secretion involved in immune response immune response-inhibiting cell surface receptor signaling pathway Fc receptor mediated inhibitory signaling pathway signal transduction response to virus positive regulation of gene expression negative regulation of serotonin secretion receptor internalization interferon-gamma production negative regulation of interferon-gamma production negative regulation of mononuclear cell proliferation negative regulation of interferon-beta secretion negative regulation of T cell proliferation negative regulation of tumor necrosis factor biosynthetic process positive regulation of apoptotic process negative regulation of interferon-gamma biosynthetic process negative regulation of cell cycle negative regulation of endocytosis positive regulation of cytolysis positive regulation of transcription from RNA polymerase II promoter negative regulation of natural killer cell mediated cytotoxicity negative regulation of alpha-beta T cell activation regulation of immune response defense response to virus negative regulation of calcium ion transport cellular response to lipopolysaccharide interferon-gamma secretion dendritic cell differentiation negative regulation of dendritic cell apoptotic process negative regulation of interleukin-10 secretion negative regulation of interleukin-12 secretion negative regulation of CD8-positive, alpha-beta T cell activation negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell positive regulation of gamma-delta T cell activation involved in immune response negative regulation of dendritic cell differentiation negative regulation of transforming growth factor-beta secretion negative regulation of osteoclast development
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