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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD86 Antibodies

          Proteintech

          CD86 Polyclonal Antibody

          116 References
          View all (187) CD86 antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
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          Cite CD86 Polyclonal Antibody

          • Antibody Testing Data (5)
          CD86 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          CD86 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          CD86 Antibody (13395-1-AP) in ICC/IF

          Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD86 antibody (Product # 13395-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD86 Antibody in Immunocytochemistry (ICC/IF)
          CD86 Antibody in Western Blot (WB)
          CD86 Antibody in Western Blot (WB)
          CD86 Antibody in Western Blot (WB)
          CD86 Antibody in Flow Cytometry (Flow)

          Product Details

          13395-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 37 publications 37 publications

          Immunohistochemistry (IHC)

          -
          View 18 publications 18 publications

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          View 51 publications 51 publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cells
          View 8 publications 8 publications

          Miscellaneous PubMed (MISC)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          CD86 Fusion Protein Ag4063 (27-247 aa encoded by BC040261)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.19 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          This antibody detects a band of approximately 70 kDa, consistent with glycosylated form of CD86.

          Immunogen sequence: KIQAYFNET ADLPCQFANS QNQSLSELVV FWQDQENLVL NEVYLGKEKF DSVHSKYMGR TSFDSDSWTL RLHNLQIKDK GLYQCIIHHK KPTGMIRIHQ MNSELSVLAN FSQPEIVPIS NITENVYINL TCSSIHGYPE PKKMSVLLRT KNSTIEYDGI MQKSQDNVTE LYDVSISLSV SFPDVTSNMT IFCILETDKT RLLSSPFSIE LEDPQPPPDH IP (27-247 aa encoded by BC040261)

          Target Information

          CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Activation B7-2 antigen; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2; B70; BU63; CD28 antigen ligand 2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; FUN-1; MGC34413; T-lymphocyte activation antigen CD86

          View more View less

          Gene Aliases: B7-2; B7.2; B70; CD28LG2; CD86; LAB72

          View more View less

          UniProt ID: (Human) P42081

          View more View less

          Entrez Gene ID: (Human) 942

          View more View less

          Function(s)
          virus receptor activity receptor activity receptor binding protein binding coreceptor activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity
          Process(es)
          response to yeast adaptive immune response T cell proliferation involved in immune response negative regulation of T cell anergy immune response cell-cell signaling aging positive regulation of cell proliferation regulation of phosphatidylinositol 3-kinase signaling T cell costimulation toll-like receptor 3 signaling pathway response to interferon-gamma positive regulation of activated T cell proliferation T cell activation B cell activation response to drug myeloid dendritic cell differentiation positive regulation of lymphotoxin A biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-4 biosynthetic process positive regulation of T-helper 2 cell differentiation positive regulation of transcription, DNA-templated viral entry into host cell phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling defense response to virus cellular response to lipopolysaccharide cellular response to metal ion cellular response to cytokine stimulus
          It has to be done as per old AB suggested Products section.
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