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Invitrogen
CD86 Monoclonal Antibody (BU63), APC
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Product Details
MA1-10294
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
BU63
Immunogen
B-lymphoblastoid cell line ARH 77
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11154656
Product Specific Information
This antibody reacts with an extracellular epitope of CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Target Information
CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activation B7-2 antigen; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2; B70; BU63; CD28 antigen ligand 2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; FUN-1; MGC34413; T-lymphocyte activation antigen CD86
Gene Aliases: B7-2; B7.2; B70; CD28LG2; CD86; LAB72
UniProt ID: (Human) P42081
Entrez Gene ID: (Human) 942
response to yeast
adaptive immune response
T cell proliferation involved in immune response
negative regulation of T cell anergy
immune response
cell-cell signaling
aging
positive regulation of cell proliferation
regulation of phosphatidylinositol 3-kinase signaling
T cell costimulation
toll-like receptor 3 signaling pathway
response to interferon-gamma
positive regulation of activated T cell proliferation
T cell activation
B cell activation
response to drug
myeloid dendritic cell differentiation
positive regulation of lymphotoxin A biosynthetic process
positive regulation of interleukin-2 biosynthetic process
positive regulation of interleukin-4 biosynthetic process
positive regulation of T-helper 2 cell differentiation
positive regulation of transcription, DNA-templated
viral entry into host cell
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
defense response to virus
cellular response to lipopolysaccharide
cellular response to metal ion
cellular response to cytokine stimulus
Performance Guarantee
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