Invitrogen

CD86 Monoclonal Antibody (GL-1)

1 Published Figure

13 References

View all (200) CD86 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (1)

FIGURE: 1 / 3

CD86 Antibody (MA1-10299) in Flow

Separation of murine CD86 positive myeloid cells (red-filled) from murine CD86 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of mouse peritoneum fluid cells suspension stained using anti-mouse CD86 (GL-1) purified Monoclonal antibody (Product # MA1-10299) (low endotoxin, concentration in sample 0.6 µg/mL) DAR APC.

CD86 Antibody in Immunocytochemistry (ICC/IF)

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Product Details

MA1-10299

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

2 µg/mL

View 8 publications 8 publications

Immunoprecipitation (IP)

Assay-dependent

Functional Assay (Functional)

Assay-dependent

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

GL-1

Immunogen

LPS-activated CBA/Cs mouse splenic B cells

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11152324

Product Specific Information

This antibody reacts with an extracellular epitope of CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

Functional Application: Blocking.

Target Information

CD86, along with CD80, is a member of the B7 family of costimulatory molecules and plays a crucial role in T cell activation and immune response regulation. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and its expression is upregulated on B cells through various stimuli, including the BCR complex, CD40, and certain cytokine receptors. As a type I membrane protein and member of the immunoglobulin superfamily, CD86 serves as a ligand for the T cell surface proteins CD28 and CTLA-4 (CD152). The interaction between CD86 and CD28 provides a costimulatory signal essential for T cell activation during antigen presentation, while binding with CTLA-4 negatively regulates T cell activation, diminishing the immune response. This interaction is critical for T-B cell crosstalk, T cell costimulation, autoantibody production, and Th2-mediated Ig production. The kinetics of CD86 upregulation upon stimulation suggest its significant contribution during the primary phase of an immune response. CD86 and CD80 have distinct roles in T helper cell differentiation, and insufficient co-stimulation involving these molecules can induce tolerance. Alternative splicing of CD86 results in two transcript variants encoding different isoforms, with additional variants described but not fully sequenced. Dysfunction in CD86 is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

OX40 agonism enhances PD-L1 checkpoint blockade by shifting the cytotoxic T cell differentiation spectrum.

Cell reports. Medicine

van der Sluis TC,Beyrend G,van der Gracht ETI,Abdelaal T,Jochems SP,Belderbos RA,Wesselink TH,van Duikeren S,van Haften FJ,Redeker A,Ouboter LF,Beyranvand Nejad E,Camps M,Franken KLMC,Linssen MM,Hohenstein P,de Miranda NFCC,Mei H,Bins AD,Haanen JBAG,Aerts JG,Ossendorp F,Arens R

MA1-10299 was used in Flow Cytometry to interrogate whether the landscape of T cell states in the peripheral blood predict responses to combinatorial targeting of the OX40 costimulatory and PD-1 inhibitory pathways.

Tue Mar 21 00:00:00 UTC 2023

TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage.

Frontiers in immunology

Wu X,Zeng H,Xu C,Chen H,Fan L,Zhou H,Yu Q,Fu X,Peng Y,Yan F,Yu X,Chen G

MA1-10299 was used in Immunocytochemistry, Immunohistochemistry to illustrate the correlation between post-SAH (subarachnoid hemorrhage) triggering receptor expressed on myeloid cells 1 (TREM1) plasma levels and SAH severity, show attenuation of neuroinflammation and defects with TREM1 inhibitory peptide LP17, and elucidate associated mechanisms.

Fri Feb 18 00:00:00 UTC 2022

Frontiers in immunology

Wu X,Zeng H,Xu C,Chen H,Fan L,Zhou H,Yu Q,Fu X,Peng Y,Yan F,Yu X,Chen G

Fri Feb 18 00:00:00 UTC 2022

Critical role of post-transcriptional regulation for IFN-γ in tumor-infiltrating T cells.

Oncoimmunology

Salerno F,Guislain A,Freen-Van Heeren JJ,Nicolet BP,Young HA,Wolkers MC

MA1-10299 was used in Flow cytometry/Cell sorting to conclude that post-transcriptional mechanisms could be modulated to potentiate effective T cell therapies in cancer.

Tue Jan 12 00:00:00 UTC 2021

PD-1 Primarily Targets TCR Signal in the Inhibition of Functional T Cell Activation.

Frontiers in immunology

Mizuno R,Sugiura D,Shimizu K,Maruhashi T,Watada M,Okazaki IM,Okazaki T

MA1-10299 was used in Flow cytometry/Cell sorting to indicate that PD-1 primarily targets TCR signal in the inhibition of functional T cell activation.

Tue Aug 04 00:00:00 UTC 2020

C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells.

Proceedings of the National Academy of Sciences of the United States of America

Troegeler A,Mercier I,Cougoule C,Pietretti D,Colom A,Duval C,Vu Manh TP,Capilla F,Poincloux R,Pingris K,Nigou J,Rademann J,Dalod M,Verreck FA,Al Saati T,Lugo-Villarino G,Lepenies B,Hudrisier D,Neyrolles O

MA110299 was used in flow cytometry to find that C-type lectin dendritic cell immunoreceptor is required to modulate lung inflammation and bacterial burden in tuberculosis

Tue Jan 24 00:00:00 UTC 2017

Vitamin D reduces high-fat diet induced weight gain and C-reactive protein, increases interleukin-10, and reduces CD86 and caspase-3.

Pathophysiology : the official journal of the International Society for Pathophysiology

Gomaa AM,El-Aziz EA

MA110299 was used in immunohistochemistry to study the effects of a high-fat diet on the spleen and immune system and to determine the protective effects of chronic treatment with vitamin D

Wed Mar 01 00:00:00 UTC 2017

TLR-exosomes exhibit distinct kinetics and effector function.

Scientific reports

Srinivasan S,Su M,Ravishankar S,Moore J,Head P,Dixon JB,Vannberg F

MA110299 was used in immunohistochemistry - frozen section to report the differential effector function by exosomes in communicating the toll like receptor activation state of the original activated cell

Tue Mar 14 00:00:00 UTC 2017

CXCL16-positive dendritic cells enhance invariant natural killer T cell-dependent IFNγ production and tumor control.

Oncoimmunology

Veinotte L,Gebremeskel S,Johnston B

MA110299 was used in flow cytometry to study the contribution of CXCR6/CXCL16 interactions in glycolipid-dependent invariant natural killer cell activation and tumor control

Wed Jun 01 00:00:00 UTC 2016

Lymphocytic Microparticles Modulate Angiogenic Properties of Macrophages in Laser-induced Choroidal Neovascularization.

Scientific reports

Tahiri H,Omri S,Yang C,Duhamel F,Samarani S,Ahmad A,Vezina M,Bussières M,Vaucher E,Sapieha P,Hickson G,Hammamji K,Lapointe R,Rodier F,Tremblay S,Royal I,Cailhier JF,Chemtob S,Hardy P

MA110299 was used in flow cytometry to study the ability of lymphocyte-derived microparticles to modulate the gene expression pattern of angiogenesis-related factors in macrophages

Tue Nov 22 00:00:00 UTC 2016

Inhibition of the TNF Family Cytokine RANKL Prevents Autoimmune Inflammation in the Central Nervous System.

Immunity

Guerrini MM,Okamoto K,Komatsu N,Sawa S,Danks L,Penninger JM,Nakashima T,Takayanagi H

MA1-10299 was used in Flow Cytometry to show that receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL) on T cells regulates C-C type chemokine ligand 20 (CCL20) production by astrocytes and T cell localization in the CNS.

Tue Dec 15 00:00:00 UTC 2015

Inflammatory neovascularization during graft-versus-host disease is regulated by αv integrin and miR-100.

Blood

Leonhardt F,Grundmann S,Behe M,Bluhm F,Dumont RA,Braun F,Fani M,Riesner K,Prinz G,Hechinger AK,Gerlach UV,Dierbach H,Penack O,Schmitt-Gräff A,Finke J,Weber WA,Zeiser R

MA1-10299 was used in Flow Cytometry to identify negative regulation of GvHD-related neovascularization by miR-100.

Thu Apr 25 00:00:00 UTC 2013

Targeted inactivation of the tetraspanin CD37 impairs T-cell-dependent B-cell response under suboptimal costimulatory conditions.

Molecular and cellular biology

Knobeloch KP,Wright MD,Ochsenbein AF,Liesenfeld O,Löhler J,Zinkernagel RM,Horak I,Orinska Z

MA1-10299 was used in flow cytometry to generate and characterize mice deficient for CD37.

Tue Aug 01 00:00:00 UTC 2000

Bioinformatics

Protein Aliases: Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD86; early T cell costimulatory molecule-1; Early T-cell co-stimulatory molecule 1; Early T-cell costimulatory molecule 1; ETC-1; MGC34413; T-lymphocyte activation antigen CD86

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Gene Aliases: B7; B7-2; B7.2; B70; Cd28l2; Cd86; CLS1; ETC-1; Ly-58; Ly58; MB7; MB7-2; TS/A-2

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UniProt ID: (Mouse) P42082

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Entrez Gene ID: (Mouse) 12524

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Function(s)

receptor binding

Process(es)

toll-like receptor signaling pathway immune system process negative regulation of T cell anergy positive regulation of T cell proliferation positive regulation of activated T cell proliferation B cell activation defense response to virus cellular response to lipopolysaccharide

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD86 Monoclonal Antibody (GL-1)

CD86 Antibody (MA1-10299) in Flow

Product Details

MA1-10299

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Functional Assay (Functional)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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