Invitrogen

CD86 (B7-2) Monoclonal Antibody (24F), PE, eBioscience™

3 Published Figures

6 References

View all (200) CD86 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (3)

CD86 (B7-2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 4

CD86 (B7-2) Antibody (12-0860-83) in Flow

Staining of LPS-stimulated rat splenocytes with staining buffer (autofluorescence) (open histogram) and Anti-Rat CD86 (B7-2) FITC (Product # 11-0860-82) (left, filled histogram) and 0.125 µg (right, filled histogram) Anti-Rat CD86 (B7-2) PE. Total viable cells were used for analysis.

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Product Details

12-0860-83

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 6 publications 6 publications

Product Specifications

Species Reactivity

Rat

Published species

Rat

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

24F

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_465763

Product Specific Information

Description: The 24F monoclonal antibody reacts with rat CD86, an ~90 kDa surface receptor, also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed by germinal center B cells and at low levels on macrophages, dendritic cells and non-lymphoid cells in the thymus. Its expression is upregulated on B cells through a variety of surface stimuli including LPS. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.

Applications Reported: The 24F antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 24F antibody has been tested by flow cytometric analysis of resting and activated rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD86, along with CD80, is a member of the B7 family of costimulatory molecules and plays a crucial role in T cell activation and immune response regulation. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and its expression is upregulated on B cells through various stimuli, including the BCR complex, CD40, and certain cytokine receptors. As a type I membrane protein and member of the immunoglobulin superfamily, CD86 serves as a ligand for the T cell surface proteins CD28 and CTLA-4 (CD152). The interaction between CD86 and CD28 provides a costimulatory signal essential for T cell activation during antigen presentation, while binding with CTLA-4 negatively regulates T cell activation, diminishing the immune response. This interaction is critical for T-B cell crosstalk, T cell costimulation, autoantibody production, and Th2-mediated Ig production. The kinetics of CD86 upregulation upon stimulation suggest its significant contribution during the primary phase of an immune response. CD86 and CD80 have distinct roles in T helper cell differentiation, and insufficient co-stimulation involving these molecules can induce tolerance. Alternative splicing of CD86 results in two transcript variants encoding different isoforms, with additional variants described but not fully sequenced. Dysfunction in CD86 is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Hydrangea paniculata coumarins attenuate experimental membranous nephritis by bidirectional interactions with the gut microbiota.

Communications biology

Li Z,Zhang X,Wu H,Ma Z,Liu X,Ma J,Zhang D,Sheng L,Chen X,Zhang S

12-0860-83 was used in Flow Cytometry to demonstrate that deteriorated renal function and gut dysbiosis are found in MN rats, as manifested by a higher Firmicutes/Bacteroidetes ratio and reduced diversity and richness, but both changes were reversed by HP treatment.

Wed Nov 22 00:00:00 UTC 2023

Acinar Cell-Derived Extracellular Vesicle MiRNA-183-5p Aggravates Acute Pancreatitis by Promoting M1 Macrophage Polarization Through Downregulation of FoxO1.

Frontiers in immunology

Tang DS,Cao F,Yan CS,Cui JT,Guo XY,Cheng L,Li L,Li YL,Ma JM,Fang K,Gao L,Ren NS,Sun B,Wang G,Ji L

Published figure using CD86 (B7-2) monoclonal antibody (Product # 12-0860-83) in Flow Cytometry

Tue Aug 02 00:00:00 UTC 2022

Transplanted allogeneic cardiac progenitor cells secrete GDF-15 and stimulate an active immune remodeling process in the ischemic myocardium.

Journal of translational medicine

Mishra R,Saha P,Datla SR,Mellacheruvu P,Gunasekaran M,Guru SA,Fu X,Chen L,Bolli R,Sharma S,Kaushal S

12-0860-83 was used in Flow cytometry/Cell sorting to investigate if transplanted allogenic rat cardiac progenitor cells (rCPCs) augment T-regulatory cells which ultimately promote proliferation of M2 like macrophages by an as-yet undefined mechanism.

Thu Jul 21 00:00:00 UTC 2022

Qing-Luo-Yin Alleviated Experimental Arthritis in Rats by Disrupting Immune Feedback Between Inflammatory T Cells and Monocytes: Key Evidences from Its Effects on Immune Cell Phenotypes.

Journal of inflammation research

Wang DD,Wu XY,Dong JY,Cheng XP,Gu SF,Olatunji OJ,Li Y,Zuo J

12-0860-83 was used in Flow Cytometry to investigate the changes in CD4+ T cells and monocytes in Qing-Luo-Yin-treated rheumatoid arthritis animal models.

Fri Apr 05 00:00:00 UTC 2024

Qing-Luo-Yin Alleviated Experimental Arthritis in Rats by Disrupting Immune Feedback Between Inflammatory T Cells and Monocytes: Key Evidences from Its Effects on Immune Cell Phenotypes.

Journal of inflammation research

Wang DD,Wu XY,Dong JY,Cheng XP,Gu SF,Olatunji OJ,Li Y,Zuo J

12-0860-83 was used in Flow Cytometry to investigate the changes in CD4+ T cells and monocytes in Qing-Luo-Yin-treated rheumatoid arthritis animal models.

Fri Apr 05 00:00:00 UTC 2024

M1 Macrophage Derived Exosomes Aggravate Experimental Autoimmune Neuritis via Modulating Th1 Response.

Frontiers in immunology

Du T,Yang CL,Ge MR,Liu Y,Zhang P,Li H,Li XL,Li T,Liu YD,Dou YC,Yang B,Duan RS

12-0860 was used in Flow cytometry/Cell sorting to determine the effects of macrophage derived exosomes on the development of EAN in Lewis rats.

Tue Apr 13 00:00:00 UTC 2021

Amelioration of collagen-induced arthritis using antigen-loaded dendritic cells modified with NF-κB decoy oligodeoxynucleotides.

Drug design, development and therapy

Jiang H,Hu H,Zhang Y,Yue P,Ning L,Zhou Y,Shi P,Yuan R

Published figure using CD86 (B7-2) monoclonal antibody (Product # 12-0860-83) in Flow Cytometry

Mon Jul 23 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: B lymphocyte activation antigen B72; CD28 antigen ligand 2; cd86 antigen; Early T-cell co-stimulatory molecule 1; membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86

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Gene Aliases: B7-2

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Entrez Gene ID: (Rat) 56822

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Function(s)

receptor binding

Process(es)

response to yeast toll-like receptor signaling pathway T cell proliferation involved in immune response negative regulation of T cell anergy aging response to lipopolysaccharide response to interferon-gamma positive regulation of T cell proliferation positive regulation of activated T cell proliferation B cell activation response to drug myeloid dendritic cell differentiation defense response to virus cellular response to lipopolysaccharide cellular response to metal ion cellular response to cytokine stimulus

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CD86 (B7-2) Monoclonal Antibody (24F), PE, eBioscience™

CD86 (B7-2) Antibody (12-0860-83) in Flow

Product Details

12-0860-83

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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