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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD86 Antibodies

          Invitrogen

          CD86 (B7-2) Monoclonal Antibody (GL1), Super Bright™ 702, eBioscience™

          81 Published Figures
          69 References
          View all (187) CD86 antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD86 (B7-2) Monoclonal Antibody (GL1), Super Bright™ 702, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (81)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 82

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          CD86 (B7-2) Antibody (67-0862-82) in Flow

          C57BL/6 mouse splenocytes were unstimulated (left) or stimulated for 72 hours with Lipopolysaccharide (LPS) Solution (500X) (Product # 00-4976) (right). Cells were then stained with CD19 Monoclonal Antibody, eFluor 450 (Product # 48-0193-82) and 0.125 µg of CD86 (B7-2) Monoclonal Antibody, Super Bright 702. Cells in the lymphocyte gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Immunocytochemistry (ICC/IF)
          CD86 (B7-2) Antibody in Immunocytochemistry (ICC/IF)
          CD86 (B7-2) Antibody in Immunohistochemistry (IHC)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)
          CD86 (B7-2) Antibody in Flow Cytometry (Flow)

          Product Details

          67-0862-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 2 publications 2 publications

          Flow Cytometry (Flow)

          0.25 µg/test
          View 66 publications 66 publications
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Recommended Isotype Control

          Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 702, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          GL1

          Conjugate

          Super Bright™ 702 Super Bright™ 702 Super Bright™ 702
          • Unconjugated
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 395
          • Brilliant UV 737
          • Brilliant Violet 605
          • Brilliant Violet 786
          • eFluor 450
          • FITC
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Request custom conjugation

          Excitation/Emission Max

          413/702 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2717155

          Product Specific Information

          Description: The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response.

          Applications Reported: This GL1 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This GL1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

          When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

          Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

          Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

          Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser

          Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

          Target Information

          CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD86; early T cell costimulatory molecule-1; Early T-cell co-stimulatory molecule 1; Early T-cell costimulatory molecule 1; ETC-1; MGC34413; T-lymphocyte activation antigen CD86

          View more View less

          Gene Aliases: B7; B7-2; B7.2; B70; Cd28l2; Cd86; CLS1; ETC-1; Ly-58; Ly58; MB7; MB7-2; TS/A-2

          View more View less

          UniProt ID: (Mouse) P42082

          View more View less

          Entrez Gene ID: (Mouse) 12524

          View more View less

          Function(s)
          receptor binding
          Process(es)
          toll-like receptor signaling pathway immune system process negative regulation of T cell anergy positive regulation of T cell proliferation positive regulation of activated T cell proliferation B cell activation defense response to virus cellular response to lipopolysaccharide
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          We're here to help

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