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Invitrogen
CD86 (B7-2) Monoclonal Antibody (IT2.2), Alexa Fluor™ 700, eBioscience™
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FIGURE: 1 / 17
CD86 (B7-2) Antibody (56-0869-42) in Flow
Normal human peripheral blood cells were stained with CD14 Monoclonal Antibody, FITC (Product # 11-0149-42) and Mouse IgG2b kappa Isotype Control, Alexa Fluor 700 (Product # 56-4732-80) (left) or CD86 (B7-2) Monoclonal Antibody, Alexa Fluor 700 (right). Cells in the monocyte gate were used for analysis.
Product Details
56-0869-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
IT2.2
Conjugate
Excitation/Emission Max
696/719 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2811891
Product Specific Information
Description: The IT2.2 monoclonal antibody reacts with human CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.
Applications Reported: This IT2.2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This IT2.2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser
Target Information
CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: Activation B7-2 antigen; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2; B70; BU63; CD28 antigen ligand 2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; FUN-1; MGC34413; T-lymphocyte activation antigen CD86
Gene Aliases: B7-2; B7.2; B70; CD28LG2; CD86; LAB72
UniProt ID: (Human) P42081
Entrez Gene ID: (Human) 942
response to yeast
adaptive immune response
T cell proliferation involved in immune response
negative regulation of T cell anergy
immune response
cell-cell signaling
aging
positive regulation of cell proliferation
regulation of phosphatidylinositol 3-kinase signaling
T cell costimulation
toll-like receptor 3 signaling pathway
response to interferon-gamma
positive regulation of activated T cell proliferation
T cell activation
B cell activation
response to drug
myeloid dendritic cell differentiation
positive regulation of lymphotoxin A biosynthetic process
positive regulation of interleukin-2 biosynthetic process
positive regulation of interleukin-4 biosynthetic process
positive regulation of T-helper 2 cell differentiation
positive regulation of transcription, DNA-templated
viral entry into host cell
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
defense response to virus
cellular response to lipopolysaccharide
cellular response to metal ion
cellular response to cytokine stimulus
Performance Guarantee
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