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Invitrogen
CD87 (UPAR) Monoclonal Antibody (VIM5), PerCP-eFluor™ 710, eBioscience™
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Product Details
46-3879-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
VIM5
Conjugate
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573753
Product Specific Information
Description: This VIM5 monoclonal antibody reacts with human CD87, a GPI-linked receptor for urokinase plasminogen activator (uPA). By sequestering uPA, CD87 is involved in the conversion of plasminogen to plasmin on the leading edge of migrating cells, a process critical for chemotaxis. CD87 is expressed on the surface of monocytes, dendritic cells, and on activated T and NK cells. Interestingly, in granulocytes, CD87 expressed in the cytoplasm and on the cell surface. Non-hematopoietic cells such as endothelial cells, keratinocytes, and fibroblasts, also express this receptor. Finally, CD87 plays a role in cell adhesion and migration, angiogenesis, and tumor metastasis.
This monoclonal antibody has been reported to have neutralization activity.
Applications Reported: This VIM5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This VIM5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The urokinase-type plasminogen activator receptor is a key molecule in the regulation of cell-surface plasminogen activation and plays an important role in many normal as well as pathologic processes. The human PLAUR cDNA encodes 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. It produces a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. PLAUR, also known as UPAR, is directly associated with the carbohydrate-binding domain of SELL in the membrane of neutrophils, an association analogous to that between PLAUR and beta-2 integrins. PLAUR-mediated calcium mobilization is SELL dependent. UPAR mRNA levels correlate with the invasive potential of endometrial carcinomas and show a 33-fold increase in UPAR mRNA levels in advanced clinical stage endometrial tumors compared with normal endometrial tissue. Furthermore, the increase in UPAR mRNA levels correlated linearly with the progression of disease stage. UPAR protein expressioin correlated positively with rate of recurrence and mortality in patients with endometrial cancer. UPAR appears to be a useful prognostic marker for advanced endometrial cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD87; Monocyte activation antigen Mo3; sCD87; soluble CD87; U-PAR; u-plasminogen activator receptor form 2; Urokinase plasminogen activator surface receptor; urokinase-type plasminogen activator (uPA) receptor
Gene Aliases: CD87; MO3; PLAUR; U-PAR; UPAR; URKR
UniProt ID: (Human) Q03405
Entrez Gene ID: (Human) 5329
positive regulation of protein phosphorylation
movement of cell or subcellular component
chemotaxis
signal transduction
blood coagulation
attachment of GPI anchor to protein
regulation of proteolysis
urokinase plasminogen activator signaling pathway
fibrinolysis
negative regulation of apoptotic process
positive regulation of DNA binding
positive regulation of epidermal growth factor receptor signaling pathway
positive regulation of release of cytochrome c from mitochondria
negative regulation of intrinsic apoptotic signaling pathway
negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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