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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD8 Antibodies

          Invitrogen

          CD8a Monoclonal Antibody (53-6.7), Biotin, Functional Grade, eBioscience™

          71 Published Figures
          168 References
          View all (529) CD8 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite CD8a Monoclonal Antibody (53-6.7), Biotin, Functional Grade, eBioscience™

          • Published Figures (71)
          CD8a Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          CD8a Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 71

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          CD8a Antibody (36-0081-85) in WB

          eLife 2019 - Figure 1. Reticuloendothelial tissues accumulate dark pigments in the absence of SLC48A1 . ( A ) Structure of the SLC48A1 gene (which encodes SLC48A1) indicating the CRISPR target site in exon 1. ( B ) Predicted topology of SLC48A1 protein; arrow indicates the site of the two basepair deletion resulting in frameshift mutation. ( C ) Immunoblot analysis of membrane lysates prepared from spleens and livers of... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD8a Antibody in Western Blot (WB)
          CD8a Antibody in Western Blot, Flow Cytometry (WB, Flow)
          CD8a Antibody in Immunocytochemistry (ICC/IF)
          CD8a Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD8a Antibody in Immunocytochemistry (ICC/IF)
          CD8a Antibody in Immunocytochemistry (ICC/IF)
          CD8a Antibody in Immunocytochemistry (ICC/IF)
          CD8a Antibody in Immunocytochemistry (ICC/IF)
          CD8a Antibody in Immunocytochemistry (ICC/IF)
          CD8a Antibody in Immunohistochemistry (IHC)
          CD8a Antibody in Immunohistochemistry (IHC)
          CD8a Antibody in Immunohistochemistry (IHC)
          CD8a Antibody in Immunohistochemistry (IHC)
          CD8a Antibody in Immunohistochemistry (IHC)
          CD8a Antibody in Immunohistochemistry (IHC)
          CD8a Antibody in Immunohistochemistry (IHC)
          CD8a Antibody in Immunohistochemistry (IHC)
          CD8a Antibody in Immunohistochemistry (IHC)
          CD8a Antibody in Immunohistochemistry (IHC)
          CD8a Antibody in Immunohistochemistry (IHC)
          CD8a Antibody in Immunohistochemistry (IHC)
          CD8a Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          CD8a Antibody in Immunohistochemistry (IHC)
          CD8a Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          CD8a Antibody in Immunohistochemistry (IHC)
          CD8a Antibody in Immunohistochemistry (IHC)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)
          CD8a Antibody in Flow Cytometry (Flow)

          Product Details

          36-0081-85

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          -
          View 17 publications 17 publications

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-Dependent
          -

          Immunocytochemistry (ICC/IF)

          -
          View 6 publications 6 publications

          Flow Cytometry (Flow)

          0.5 µg/test
          View 140 publications 140 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          Functional Assay (Functional)

          Assay-Dependent
          View 2 publications 2 publications

          Control (Ctrl)

          Assay-Dependent
          -
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Hamster, Human, Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          53-6.7

          Conjugate

          Biotin , Functional Grade Biotin , Functional Grade Biotin , Functional Grade

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_469749

          Product Specific Information

          Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alphaalpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gammadelta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alphaalpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

          Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.

          Applications Tested: This 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Endotoxin: Less than 0.05 ng/µg antibody as determined by the LAL assay.

          Storage and handling: Use in a sterile environment.

          Target Information

          Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3

          View more View less

          Gene Aliases: BB154331; Cd8a; Cd8b; Cd8b1; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; Lyt-2; Lyt-3; Lyt2; Lyt3

          View more View less

          UniProt ID: (Mouse) P01731, (Mouse) P10300

          View more View less

          Entrez Gene ID: (Mouse) 12525, (Mouse) 12526

          View more View less

          Function(s)
          protein binding protein kinase binding protein homodimerization activity
          Process(es)
          immune system process T cell mediated immunity cell surface receptor signaling pathway T cell activation cytotoxic T cell differentiation positive regulation of calcium-mediated signaling defense response to virus
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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