Invitrogen

CD9 Recombinant Rabbit Monoclonal Antibody (SA35-08)

6 Published Figures

8 References

View all (71) CD9 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (8)
Published Figures (6)

CD9 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

CD9 Antibody (MA5-31980) in ICC/IF

Immunocytochemical analysis of CD9 in SW480 cells using a CD9 Monoclonal antibody (Product # MA5-31980) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

CD9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

MA5-31980

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:1,000

Immunocytochemistry (ICC/IF)

1:50

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SA35-08

Immunogen

Synthetic peptide within Human CD9 aa 179-228

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2809274

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis. CD9 is also considered as metastasis suppressor in solid tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Therapeutic role of adipose-derived mesenchymal stem cells-derived extracellular vesicles in rats with obstructive sleep apnea hypopnea syndrome.

Regenerative therapy

Xu L,Zhang L,Xiang Y,Zhang X

MA5-31980 was used in Western Blotting to find that ADSCs-EVs transferred miR-22-3p to pneumonocytes and reduced apoptosis, inflammation, and oxidative stress through KDM6B/HMGA2, mitigating OSAHS-LI progression.

Wed Mar 01 00:00:00 UTC 2023

Therapeutic role of adipose-derived mesenchymal stem cells-derived extracellular vesicles in rats with obstructive sleep apnea hypopnea syndrome.

Regenerative therapy

Xu L,Zhang L,Xiang Y,Zhang X

Wed Mar 01 00:00:00 UTC 2023

Extracellular Vesicle-Encapsulated MicroRNA-375 from Bone Marrow-Derived Mesenchymal Stem Cells Inhibits Hepatocellular Carcinoma Progression through Regulating HOXB3-Mediated Wnt/β-Catenin Pathway.

Analytical cellular pathology (Amsterdam)

Yu Z,Liu J,Fan Q,Yu J,Ren X,Wang X

Published figure using CD9 recombinant monoclonal antibody (Product # MA5-31980) in Western Blot

Mon Apr 04 00:00:00 UTC 2022

Characterization of plasma exosomal microRNAs in responding to radiotherapy of human esophageal squamous cell carcinoma.

Molecular medicine reports

Miao N,Cai W,Ding S,Liu Y,Chen W,Sun T

MA5-31980 was used in Western Blotting to identify potential exosomal miRNAs that respond to radiotherapy, plasma exosomal miRNAs from ESCC patients undergoing radiotherapy were isolated and sequenced.

Thu Sep 01 00:00:00 UTC 2022

Characterization of plasma exosomal microRNAs in responding to radiotherapy of human esophageal squamous cell carcinoma.

Molecular medicine reports

Miao N,Cai W,Ding S,Liu Y,Chen W,Sun T

Thu Sep 01 00:00:00 UTC 2022

Cell death & disease

Zhang M,Gong W,Zhang D,Ji M,Chen B,Chen B,Li X,Zhou Y,Dong C,Wen G,Zhan X,Wu X,Cui L,Feng Y,Wang S,Yuan H,Xu E,Xia M,Verkhratsky A,Li B

Published figure using CD9 recombinant monoclonal antibody (Product # MA5-31980) in Western Blot

Mon Apr 25 00:00:00 UTC 2022

A light-inducible protein clustering system for in vivo analysis of α-synuclein aggregation in Parkinson disease.

PLoS biology

Bérard M,Sheta R,Malvaut S,Rodriguez-Aller R,Teixeira M,Idi W,Turmel R,Alpaugh M,Dubois M,Dahmene M,Salesse C,Lamontagne-Proulx J,St-Pierre MK,Tavassoly O,Luo W,Del Cid-Pellitero E,Qazi R,Jeong JW,Durcan TM,Vallières L,Tremblay ME,Soulet D,Lévesque M,Cicchetti F,Fon EA,Saghatelyan A,Oueslati A

MA5-31980 was used in Immunocytochemistry-immunofluorescence to provide a new tool for the generation, visualization, and dissection of the role of α-syn aggregation in PD.

Tue Mar 01 00:00:00 UTC 2022

Genetic Background and Kinetics Define Wound Bed Extracellular Vesicles in a Mouse Model of Cutaneous Injury.

International journal of molecular sciences

Qian J,Park DJ,Perrott S,Patel P,Eliceiri BP

Published figure using CD9 recombinant monoclonal antibody (Product # MA5-31980) in Western Blot

Mon Mar 29 00:00:00 UTC 2021

Macrophage secretion of miR-106b-5p causes renin-dependent hypertension.

Nature communications

Oh J,Matkovich SJ,Riek AE,Bindom SM,Shao JS,Head RD,Barve RA,Sands MS,Carmeliet G,Osei-Owusu P,Knutsen RH,Zhang H,Blumer KJ,Nichols CG,Mecham RP,Baldán Á,Benitez BA,Sequeira-Lopez ML,Gomez RA,Bernal-Mizrachi C

MA5-31980 was used in Western Blotting to confirm that macrophage miR-106b-5p secretion from impaired vitamin D receptor signalling causes inflammation-induced hypertension.

Wed Sep 23 00:00:00 UTC 2020

Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis.

Nature communications

Dinh PC,Paudel D,Brochu H,Popowski KD,Gracieux MC,Cores J,Huang K,Hensley MT,Harrell E,Vandergriff AC,George AK,Barrio RT,Hu S,Allen TA,Blackburn K,Caranasos TG,Peng X,Schnabel LV,Adler KB,Lobo LJ,Goshe MB,Cheng K

MA5-31980 was used in Western Blotting to show that an inhalation treatment of secretome and exosome exhibited therapeutic potential for lung regeneration in two experimental models of pulmonary fibrosis.

Fri Feb 28 00:00:00 UTC 2020

Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis.

Nature communications

Fri Feb 28 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: 5H9; 5H9 antigen; antigen CD9; BA-2/p24 antigen; BA2; CD9; CD9 antigen; CD9 antigen (p24); Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; motility related protein-1; Motility-related protein; MRP-1; p24; sCD 9; sCD9; soluble CD 9; soluble CD9; Tetraspanin-29; Tetraspanin29; Tspan-29

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Gene Aliases: BTCC-1; CD9; DRAP-27; GIG2; MIC3; MRP-1; TSPAN-29; TSPAN29

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UniProt ID: (Human) P21926, (Mouse) P40240, (Rat) P40241

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Entrez Gene ID: (Human) 928, (Mouse) 12527, (Rat) 24936

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Function(s)

integrin binding protein binding

Process(es)

movement of cell or subcellular component cell adhesion single fertilization fusion of sperm to egg plasma membrane negative regulation of cell proliferation paranodal junction assembly platelet degranulation cell surface receptor signaling pathway brain development response to water deprivation oligodendrocyte development platelet activation receptor internalization cellular response to low-density lipoprotein particle stimulus

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CD9 Recombinant Rabbit Monoclonal Antibody (SA35-08)

CD9 Antibody (MA5-31980) in ICC/IF

Product Details

MA5-31980

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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