Invitrogen

CD9 Monoclonal Antibody (eBioKMC8 (KMC8)), PE, eBioscience™

10 Published Figures

15 References

View all (71) CD9 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (10)

FIGURE: 1 / 11

CD9 Antibody (12-0091-81) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Mouse Ly-6G (Gr-1) FITC (Product # 11-5931-82) and 0.25 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (left) or Anti-Mouse CD9 PE (right). Total viable cells were used for analysis.

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Product Details

12-0091-81

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 14 publications 14 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioKMC8 (KMC8)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_891498

Product Specific Information

Description: The eBioKMC8 monoclonal antibody reacts with mouse CD9, a 24 kDa member of the transmembrane 4 superfamily. This family is characterized by the presence of four hydrophobic domains spanning the cell membrane and short N- and C-terminal cytoplasmic domains. CD9 is expressed by several cell types including monocytes, macrophages, platelets, early B cells, activated B and T cells, dendritic cells, eosinophils, basophils, endothelial cells, myoblasts and neuroblasts. On T cells, CD9 functions as a co-stimulatory molecule on naive T cells. Furthermore, CD9 is expressed in oocytes, and CD9-deficiency results in sterility caused by defective gamete fusion. In mouse macrophages, CD9 functionally associates with FcgammaRs to modify signals for phagocytosis and inflammatory responses. In mouse B cells, it was discovered that CD9 is a marker for marginal zone B cells, B1 cells, and plasma cells. In dendritic cells, recently it was demonstrated that CD9 facilitates the association of heterologous MHC II molecules. The level of CD9 expression is subject to donor variability.

Applications Reported: This eBioKMC8 (KMC8) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioKMC8 (KMC8) antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis. CD9 is also considered as metastasis suppressor in solid tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Extracellular vesicles mediated exocytosis of antisense peptide nucleic acids.

Molecular therapy. Nucleic acids

Malik S,Saltzman WM,Bahal R

Published figure using CD9 monoclonal antibody (Product # 12-0091-81) in Flow Cytometry

Fri Sep 03 00:00:00 UTC 2021

Phosphatase Shp2 regulates biogenesis of small extracellular vesicles by dephosphorylating Syntenin.

Journal of extracellular vesicles

Zhang Y,Li Y,Liu P,Gong D,Zhou H,Li W,Zhang H,Zheng W,Xu J,Cheng H,Zhang X,Ke Y

Published figure using CD9 monoclonal antibody (Product # 12-0091-81) in Flow Cytometry

Mon Mar 01 00:00:00 UTC 2021

LRIG1 is a gatekeeper to exit from quiescence in adult neural stem cells.

Nature communications

Marqués-Torrejón MÁ,Williams CAC,Southgate B,Alfazema N,Clements MP,Garcia-Diaz C,Blin C,Arranz-Emparan N,Fraser J,Gammoh N,Parrinello S,Pollard SM

Published figure using CD9 monoclonal antibody (Product # 12-0091-81) in Flow Cytometry

Mon May 10 00:00:00 UTC 2021

The Depolarization-Evoked, Ca²⁺-Dependent Release of Exosomes From Mouse Cortical Nerve Endings: New Insights Into Synaptic Transmission.

Frontiers in pharmacology

Olivero G,Cisani F,Marimpietri D,Di Paolo D,Gagliani MC,Podestà M,Cortese K,Pittaluga A

12-0091-81 was used in Flow cytometry/Cell sorting to suggest that the exposure of synaptosomes to a depolarizing stimulus elicits a presynaptic release of exosomes that occurs in a Ca2+-dependent fashion.

Wed Apr 03 00:00:00 UTC 2024

The Depolarization-Evoked, Ca²⁺-Dependent Release of Exosomes From Mouse Cortical Nerve Endings: New Insights Into Synaptic Transmission.

Frontiers in pharmacology

Olivero G,Cisani F,Marimpietri D,Di Paolo D,Gagliani MC,Podestà M,Cortese K,Pittaluga A

Wed Apr 03 00:00:00 UTC 2024

Employing Flow Cytometry to Extracellular Vesicles Sample Microvolume Analysis and Quality Control.

Frontiers in cell and developmental biology

Maia J,Batista S,Couto N,Gregório AC,Bodo C,Elzanowska J,Strano Moraes MC,Costa-Silva B

12-0091-81 was used in Flow cytometry/Cell sorting to present a Flow Cytometry (FC) strategy that does not require isolation or concentration of EVs prior to staining.

Tue Nov 17 00:00:00 UTC 2020

Employing Flow Cytometry to Extracellular Vesicles Sample Microvolume Analysis and Quality Control.

Frontiers in cell and developmental biology

Maia J,Batista S,Couto N,Gregório AC,Bodo C,Elzanowska J,Strano Moraes MC,Costa-Silva B

12-0091-81 was used in Flow cytometry/Cell sorting to present a Flow Cytometry (FC) strategy that does not require isolation or concentration of EVs prior to staining.

Tue Nov 17 00:00:00 UTC 2020

A bioenergetic shift is required for spermatogonial differentiation.

Cell discovery

Chen W,Zhang Z,Chang C,Yang Z,Wang P,Fu H,Wei X,Chen E,Tan S,Huang W,Sun L,Ni T,Yang Y,Wang Y

Published figure using CD9 monoclonal antibody (Product # 12-0091-81) in Flow Cytometry

Tue Oct 03 00:00:00 UTC 2023

MFN2 Plays a Distinct Role from MFN1 in Regulating Spermatogonial Differentiation.

Stem cell reports

Chen W,Sun Y,Sun Q,Zhang J,Jiang M,Chang C,Huang X,Wang C,Wang P,Zhang Z,Chen X,Wang Y

Published figure using CD9 monoclonal antibody (Product # 12-0091-81) in Flow Cytometry

Tue May 12 00:00:00 UTC 2020

IκBε is a key regulator of B cell expansion by providing negative feedback on cRel and RelA in a stimulus-specific manner.

Journal of immunology (Baltimore, Md. : 1950)

Alves BN,Tsui R,Almaden J,Shokhirev MN,Davis-Turak J,Fujimoto J,Birnbaum H,Ponomarenko J,Hoffmann A

Published figure using CD9 monoclonal antibody (Product # 12-0091-81) in Flow Cytometry

Tue Apr 01 00:00:00 UTC 2014

The roles of testicular c-kit positive cells in de novo morphogenesis of testis.

Scientific reports

Zhang M,Zhou H,Zheng C,Xiao J,Zuo E,Liu W,Xie D,Shi Y,Wu C,Wang H,Li D,Li J

Published figure using CD9 monoclonal antibody (Product # 12-0091-81) in Flow Cytometry

Mon Aug 04 00:00:00 UTC 2014

Cigarette smoke impairs phagocytosis of apoptotic neutrophils by alveolar macrophages via inhibition of the histone deacetylase/Rac/CD9 pathways.

International immunology

Noda N,Matsumoto K,Fukuyama S,Asai Y,Kitajima H,Seki N,Matsunaga Y,Kan-O K,Moriwaki A,Morimoto K,Inoue H,Nakanishi Y

12-0091 was used in Flow cytometry/Cell sorting to investigate whether the reduction of HDAC activity in chronic obstructive pulmonary disease is associated with the impairment of efferocytosis.

Fri Nov 01 00:00:00 UTC 2013

NF-E2 p45 is important for establishing normal function of platelets.

Molecular and cellular biology

Fujita R,Takayama-Tsujimoto M,Satoh H,Gutiérrez L,Aburatani H,Fujii S,Sarai A,Bresnick EH,Yamamoto M,Motohashi H

12-0091 was used in Flow cytometry/Cell sorting to explore the role of the NF-E2 transcription factor in platelet activation.

Mon Jul 01 00:00:00 UTC 2013

Development of membrane mechanical function during terminal stages of primitive erythropoiesis in mice.

Experimental hematology

Waugh RE,Huang YS,Arif BJ,Bauserman R,Palis J

12-0091 was used in Immunocytochemistry to examine the mechanical properties of primitive erythroblasts to determine how their mechanical development differs from that of definitive erythroid cells.

Mon Apr 01 00:00:00 UTC 2013

Activated T cell exosomes promote tumor invasion via Fas signaling pathway.

Journal of immunology (Baltimore, Md. : 1950)

Cai Z,Yang F,Yu L,Yu Z,Jiang L,Wang Q,Yang Y,Wang L,Cao X,Wang J

12-0091 was used in Flow cytometry/Cell sorting to investigate the effect of FasL-containing exosomes on cell apoptosis in tumours, showing that they promote tumour invasion.

Fri Jun 15 00:00:00 UTC 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

12-0091 was used in Flow cytometry/Cell sorting to demonstrate that nuclear export of IκBα is essential in maintaining constitutive, canonical, and noncanonical NF-κB activation potentials in mature B cells in vivo.

Fri Feb 25 00:00:00 UTC 2011

CD11c expression identifies a population of extrafollicular antigen-specific splenic plasmablasts responsible for CD4 T-independent antibody responses during intracellular bacterial infection.

Journal of immunology (Baltimore, Md. : 1950)

Racine R,Chatterjee M,Winslow GM

12-0091 was used in Flow cytometry/Cell sorting to study how during this intracellular bacterial infection, CD11c expression identifies a population of Ag-specific spleen plasmablasts responsible for T-independent Ab production.

Tue Jul 15 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: 5H9; BA2; CD9; CD9 antigen; sCD 9; sCD9; soluble CD 9; soluble CD9; Tetraspanin29

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Gene Aliases: Cd9; Tspan29

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UniProt ID: (Mouse) P40240

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Entrez Gene ID: (Mouse) 12527

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Function(s)

integrin binding protein binding

Process(es)

movement of cell or subcellular component cell adhesion single fertilization fusion of sperm to egg plasma membrane negative regulation of cell proliferation paranodal junction assembly

Performance Guarantee

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CD9 Monoclonal Antibody (eBioKMC8 (KMC8)), PE, eBioscience™

CD9 Antibody (12-0091-81) in Flow

Product Details

12-0091-81

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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