Invitrogen

CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), FITC, eBioscience™

10 Published Figures

23 References

View all (48) CD90.2 (Thy1.2) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (10)

CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 11

CD90.2 (Thy-1.2) Antibody (11-0903-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e APC (Product # 17-0031-82) and 0.06 µg of Rat IgG2b kappa Isotype Control FITC (Product # 11-4031-82) (left) or 0.06 µg of Anti-Mouse CD90.2 (Thy-1.2) FITC (right). Cells in the lymphocyte gate were used for analysis.

CD90.2 (Thy-1.2) Antibody in Immunocytochemistry (ICC/IF)

CD90.2 (Thy-1.2) Antibody in Immunohistochemistry (Frozen) (IHC (F))

CD90.2 (Thy-1.2) Antibody in Immunohistochemistry (IHC)

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Product Details

11-0903-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

30-H12

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2735033

Product Specific Information

Description: The 30-H12 monoclonal antibody reacts with mouse CD90.2, also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/J, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 30-H12. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: The 30-H12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 30-H12 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lungs via CRTH2-dependent mechanisms.

The Journal of clinical investigation

Hernandez-Gutierrez A,Majid S,Eberle A,Choi A,Sorkhdini P,Yang D,Yang AX,Norbrun C,He CH,Lee CM,Lee CG,Elias JA,Zhou Y

11-0903-82 was used in Flow Cytometry to demonstrate that PLSCR1 played an essential role in the pathogenesis of ILC2 responses, providing critical insights into biology and disease pathogenesis and identifying targets that can be manipulated in attempts to control IT2IR in chronic diseases such as asthma.

Tue Aug 01 00:00:00 UTC 2023

Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.

Experimental and therapeutic medicine

Xiao K,Yang L,Xie W,Gao X,Huang R,Xie M

11-0903-82 was used in Flow Cytometry to investigate the impact of this Bcl-xL mutant on cartilage differentiation and the expression levels of TGF-β and bone morphogenetic protein (BMP).

Thu Jul 01 00:00:00 UTC 2021

Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.

Experimental and therapeutic medicine

Xiao K,Yang L,Xie W,Gao X,Huang R,Xie M

11-0903-82 was used in Flow Cytometry to investigate the impact of this Bcl-xL mutant on cartilage differentiation and the expression levels of TGF-β and bone morphogenetic protein (BMP).

Thu Jul 01 00:00:00 UTC 2021

Sex-specific adipose tissue imprinting of regulatory T cells.

Nature

Vasanthakumar A,Chisanga D,Blume J,Gloury R,Britt K,Henstridge DC,Zhan Y,Torres SV,Liene S,Collins N,Cao E,Sidwell T,Li C,Spallanzani RG,Liao Y,Beavis PA,Gebhardt T,Trevaskis N,Nutt SL,Zajac JD,Davey RA,Febbraio MA,Mathis D,Shi W,Kallies A

11-0903-82 was used in Flow Cytometry to find that sex-specific differences in Treg cells from visceral adipose tissue are determined by the tissue niche in a sex-hormone-dependent manner to limit adipose tissue inflammation.

Sun Mar 01 00:00:00 UTC 2020

Cytotoxicity of nonylphenol on spermatogonial stem cells via phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin pathway.

World journal of stem cells

Lei JH,Yan W,Luo CH,Guo YM,Zhang YY,Wang XH,Su XJ

11-0903 was used in Flow cytometry/Cell sorting to investigate the cytotoxic effect of NP on SSCs via the phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) pathway.

Fri Jun 26 00:00:00 UTC 2020

Mechanisms of interleukin 4 mediated increase in efficacy of vaccines against opioid use disorders.

NPJ vaccines

Crouse B,Robinson C,Huseby Kelcher A,Laudenbach M,Abrahante JE,Pravetoni M

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 11-0903-82) in Flow Cytometry

Thu Oct 29 00:00:00 UTC 2020

Inhibition of CXCR4 inhibits the proliferation and osteogenic potential of fibroblasts from ankylosing spondylitis via the Wnt/β‑catenin pathway.

Molecular medicine reports

He C,Li D,Gao J,Li J,Liu Z,Xu W

11-0903 was used in Flow cytometry/Cell sorting to investigate the role of C-X-C chemokine receptor type 4 (CXCR4) in ossification from patients with ankylosing spondylitis.

Mon Apr 01 00:00:00 UTC 2019

Comparison between curcumin and all-trans retinoic acid in the osteogenic differentiation of mouse bone marrow mesenchymal stem cells.

Experimental and therapeutic medicine

Ahmed MF,El-Sayed AK,Chen H,Zhao R,Yusuf MS,Zuo Q,Zhang Y,Li B

11-0903 was used in Flow cytometry/Cell sorting to examine the effects of all-trans retinoic acid and curcumin on the osteogenic differentiation of mouse bone marrow mesenchymal stem cells.

Wed May 01 00:00:00 UTC 2019

Denervation-activated STAT3-IL-6 signalling in fibro-adipogenic progenitors promotes myofibres atrophy and fibrosis.

Nature cell biology

Madaro L,Passafaro M,Sala D,Etxaniz U,Lugarini F,Proietti D,Alfonsi MV,Nicoletti C,Gatto S,De Bardi M,Rojas-García R,Giordani L,Marinelli S,Pagliarini V,Sette C,Sacco A,Puri PL

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 11-0903-82) in Immunohistochemistry

Wed Aug 01 00:00:00 UTC 2018

Denervation-activated STAT3-IL-6 signalling in fibro-adipogenic progenitors promotes myofibres atrophy and fibrosis.

Nature cell biology

Madaro L,Passafaro M,Sala D,Etxaniz U,Lugarini F,Proietti D,Alfonsi MV,Nicoletti C,Gatto S,De Bardi M,Rojas-García R,Giordani L,Marinelli S,Pagliarini V,Sette C,Sacco A,Puri PL

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 11-0903-82) in Immunohistochemistry

Wed Aug 01 00:00:00 UTC 2018

A quiescent cell population replenishes mesenchymal stem cells to drive accelerated growth in mouse incisors.

Nature communications

An Z,Sabalic M,Bloomquist RF,Fowler TE,Streelman T,Sharpe PT

Thu Jan 25 00:00:00 UTC 2018

Differentiation and Protective Capacity of Virus-Specific CD8⁺ T Cells Suggest Murine Norovirus Persistence in an Immune-Privileged Enteric Niche.

Immunity

Tomov VT,Palko O,Lau CW,Pattekar A,Sun Y,Tacheva R,Bengsch B,Manne S,Cosma GL,Eisenlohr LC,Nice TJ,Virgin HW,Wherry EJ

11-0903 was used in Flow cytometry/Cell sorting to elucidate a strategy of immune evasion by mouse norovirus via the induction of a CD8+ T cell program normally reserved for latent pathogens and persistence in an immune-privileged enteric niche.

Tue Oct 17 00:00:00 UTC 2017

Cholesterol Accumulation in Dendritic Cells Links the Inflammasome to Acquired Immunity.

Cell metabolism

Westerterp M,Gautier EL,Ganda A,Molusky MM,Wang W,Fotakis P,Wang N,Randolph GJ,D'Agati VD,Yvan-Charvet L,Tall AR

11-0903 was used in Flow cytometry to identify an essential role of DC cholesterol efflux pathways in maintaining immune tolerance.

Tue Jun 06 00:00:00 UTC 2017

Cardiac Tissue Injury and Remodeling Is Dependent Upon MR Regulation of Activation Pathways in Cardiac Tissue Macrophages.

Endocrinology

Shen JZ,Morgan J,Tesch GH,Rickard AJ,Chrissobolis S,Drummond GR,Fuller PJ,Young MJ

11-0903 was used in Flow cytometry/Cell sorting to determine the cellular mechanisms of macrophage mineralocorticoid receptor signaling in isolated tissue macrophages.

Mon Aug 01 00:00:00 UTC 2016

Transient Expression of WNT2 Promotes Somatic Cell Reprogramming by Inducing β-Catenin Nuclear Accumulation.

Stem cell reports

Kimura M,Nakajima-Koyama M,Lee J,Nishida E

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 11-0903-82) in Flow Cytometry

Tue Jun 14 00:00:00 UTC 2016

Clec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton.

eLife

Yue R,Shen B,Morrison SJ

11-0903 was used in Flow cytometry/Cell sorting to study how Clec11a/Osteolectin maintains the adult skeleton by promoting the differentiation of mesenchymal progenitors into mature osteoblasts.

Tue Dec 13 00:00:00 UTC 2016

Skin CD4(+) memory T cells exhibit combined cluster-mediated retention and equilibration with the circulation.

Nature communications

Collins N,Jiang X,Zaid A,Macleod BL,Li J,Park CO,Haque A,Bedoui S,Heath WR,Mueller SN,Kupper TS,Gebhardt T,Carbone FR

11-0903 was used in Flow cytometry/Cell sorting to asses the pattern of tissue-retention and equilibration of CD4(+) memory T cells in skin and how this is altered by infection and inflammation.

Tue May 10 00:00:00 UTC 2016

Gastric LTi cells promote lymphoid follicle formation but are limited by IRAK-M and do not alter microbial growth.

Mucosal immunology

Shiu J,Piazuelo MB,Ding H,Czinn SJ,Drakes ML,Banerjee A,Basappa N,Kobayashi KS,Fricke WF,Blanchard TG

11-0903 was used in Flow cytometry/Cell sorting to investigate the role of lymphoid tissue inducer cells in the gastric mucosa in the context of microbial infection.

Tue Sep 01 00:00:00 UTC 2015

Collaborative rewiring of the pluripotency network by chromatin and signalling modulating pathways.

Nature communications

Tran KA,Jackson SA,Olufs ZP,Zaidan NZ,Leng N,Kendziorski C,Roy S,Sridharan R

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 11-0903-82) in Flow Cytometry

Wed Feb 04 00:00:00 UTC 2015

Intronic regulation of Aire expression by Jmjd6 for self-tolerance induction in the thymus.

Nature communications

Yanagihara T,Sanematsu F,Sato T,Uruno T,Duan X,Tomino T,Harada Y,Watanabe M,Wang Y,Tanaka Y,Nakanishi Y,Suyama M,Yoshinori F

11-0903 was used in Flow cytometry/Cell sorting to study the regulation of Aire expression in medullary thymic epithelial cells.

Wed Nov 04 00:00:00 UTC 2015

Intrahepatic innate lymphoid cells secrete IL-17A and IL-17F that are crucial for T cell priming in viral infection.

Journal of immunology (Baltimore, Md. : 1950)

Jie Z,Liang Y,Hou L,Dong C,Iwakura Y,Soong L,Cong Y,Sun J

11-0903 was used in Flow cytometry/Cell sorting to investigate the source and regulation of the IL-17 surge in the liver microenvironment that occurs early in the response to viral infection.

Tue Apr 01 00:00:00 UTC 2014

Ionizing radiation-induced expression of INK4a/ARF in murine bone marrow-derived stromal cell populations interferes with bone marrow homeostasis.

Blood

Carbonneau CL,Despars G,Rojas-Sutterlin S,Fortin A,Le O,Hoang T,Beauséjour CM

11-0903 was used in Flow cytometry/Cell sorting to investigate the effect of ionizing radiation on murine bone marrow-derived stromal cell populations, and overall bone marrow homeostasis.

Thu Jan 19 00:00:00 UTC 2012

Dendritic cell-specific ablation of the protein tyrosine phosphatase Shp1 promotes Th1 cell differentiation and induces autoimmunity.

Journal of immunology (Baltimore, Md. : 1950)

Kaneko T,Saito Y,Kotani T,Okazawa H,Iwamura H,Sato-Hashimoto M,Kanazawa Y,Takahashi S,Hiromura K,Kusakari S,Kaneko Y,Murata Y,Ohnishi H,Nojima Y,Takagishi K,Matozaki T

11-0903 was used in Immunohistochemistry on frozen tissues to investigate the molecules that regulate dendritic cells to protect against autoimmunity, showing that loss of Shp1 promotes Th1 cell differentiation and induces autoimmunity.

Fri Jun 01 00:00:00 UTC 2012

Delayed, asynchronous, and reversible T-lineage specification induced by Notch/Delta signaling.

Genes & development

Taghon TN,David ES,Zúñiga-Pflücker JC,Rothenberg EV

11-0903 was used in Flow cytometry/Cell sorting to study how Notch/Delta signaling is not sufficient for T-lineage specification and commitment, but can be permissive for the maintenance and proliferation of uncommitted progenitors.

Fri Apr 15 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; Thy-1 membrane glycoprotein

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Gene Aliases: CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2

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UniProt ID: (Mouse) P01831

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Entrez Gene ID: (Mouse) 21838

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Function(s)

GTPase activator activity integrin binding enzyme binding protein kinase binding GPI anchor binding

Process(es)

angiogenesis negative regulation of protein kinase activity cytoskeleton organization single organismal cell-cell adhesion negative regulation of cell migration positive regulation of GTPase activity retinal cone cell development protein autophosphorylation focal adhesion assembly negative regulation of axonogenesis T cell receptor signaling pathway negative regulation of T cell receptor signaling pathway positive regulation of T cell activation positive regulation of release of sequestered calcium ion into cytosol

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CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), FITC, eBioscience™

CD90.2 (Thy-1.2) Antibody (11-0903-82) in Flow

Product Details

11-0903-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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