Invitrogen

CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), eBioscience™

9 Published Figures

20 References

View all (48) CD90.2 (Thy1.2) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (9)

CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 10

CD90.2 (Thy-1.2) Antibody (14-0903-82) in Flow

Staining of BALB/c splenocytes with 0.06 µg of Rat IgG2a K Isotype Control Control Purified (Product # 14-4031-82) (open histogram) or 0.06 µg of Anti-Mouse CD90-2 (Thy-1-2) Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85). Cells in the lymphocyte gate were used for analysis.

CD90.2 (Thy-1.2) Antibody in Immunocytochemistry (ICC/IF)

CD90.2 (Thy-1.2) Antibody in Immunohistochemistry (IHC)

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Product Details

14-0903-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.125 µg/test

View 12 publications 12 publications

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

30-H12

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_467382

Product Specific Information

Description: The 30-H12 monoclonal antibody reacts with mouse CD90.2, also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/J, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 30-H12. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: The 30-H12 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. It has also been reported in in vivo and in vitro depletion. (Please use Functional Grade purified 30-H12, Product # 16-0903, in functional assays).

Applications Tested: The 30-H12 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.

Experimental and therapeutic medicine

Xiao K,Yang L,Xie W,Gao X,Huang R,Xie M

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 14-0903-82) in Flow Cytometry

Thu Jul 01 00:00:00 UTC 2021

Mechanisms of interleukin 4 mediated increase in efficacy of vaccines against opioid use disorders.

NPJ vaccines

Crouse B,Robinson C,Huseby Kelcher A,Laudenbach M,Abrahante JE,Pravetoni M

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 14-0903-82) in Flow Cytometry

Thu Oct 29 00:00:00 UTC 2020

Antigen-presenting ILC3 regulate T cell-dependent IgA responses to colonic mucosal bacteria.

The Journal of experimental medicine

Melo-Gonzalez F,Kammoun H,Evren E,Dutton EE,Papadopoulou M,Bradford BM,Tanes C,Fardus-Reid F,Swann JR,Bittinger K,Mabbott NA,Vallance BA,Willinger T,Withers DR,Hepworth MR

14-0903-82 was used in Flow Cytometry to study the mechanisms that modulate the magnitude and quality of T cell-dependent IgA responses.

Mon Apr 01 00:00:00 UTC 2019

Denervation-activated STAT3-IL-6 signalling in fibro-adipogenic progenitors promotes myofibres atrophy and fibrosis.

Nature cell biology

Madaro L,Passafaro M,Sala D,Etxaniz U,Lugarini F,Proietti D,Alfonsi MV,Nicoletti C,Gatto S,De Bardi M,Rojas-García R,Giordani L,Marinelli S,Pagliarini V,Sette C,Sacco A,Puri PL

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 14-0903-82) in Immunohistochemistry

Wed Aug 01 00:00:00 UTC 2018

Denervation-activated STAT3-IL-6 signalling in fibro-adipogenic progenitors promotes myofibres atrophy and fibrosis.

Nature cell biology

Madaro L,Passafaro M,Sala D,Etxaniz U,Lugarini F,Proietti D,Alfonsi MV,Nicoletti C,Gatto S,De Bardi M,Rojas-García R,Giordani L,Marinelli S,Pagliarini V,Sette C,Sacco A,Puri PL

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 14-0903-82) in Immunohistochemistry

Wed Aug 01 00:00:00 UTC 2018

Langerin+ DCs regulate innate IL-17 production in the oral mucosa during Candida albicans-mediated infection.

PLoS pathogens

Sparber F,Dolowschiak T,Mertens S,Lauener L,Clausen BE,Joller N,Stoitzner P,Tussiwand R,LeibundGut-Landmann S

14-0903 was used in Flow cytometry/Cell sorting to study innate IL-17 dependent antifungal immunity in the oral mucosa.

Tue May 01 00:00:00 UTC 2018

A quiescent cell population replenishes mesenchymal stem cells to drive accelerated growth in mouse incisors.

Nature communications

An Z,Sabalic M,Bloomquist RF,Fowler TE,Streelman T,Sharpe PT

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 14-0903-82) in Immunofluorescence

Thu Jan 25 00:00:00 UTC 2018

A quiescent cell population replenishes mesenchymal stem cells to drive accelerated growth in mouse incisors.

Nature communications

An Z,Sabalic M,Bloomquist RF,Fowler TE,Streelman T,Sharpe PT

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 14-0903-82) in Immunofluorescence

Thu Jan 25 00:00:00 UTC 2018

The transcription factor EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction.

Nature communications

Yamamura K,Uruno T,Shiraishi A,Tanaka Y,Ushijima M,Nakahara T,Watanabe M,Kido-Nakahara M,Tsuge I,Furue M,Fukui Y

14090382 was used in flow cytometry to find that CD4+ T cells from DOCK8-deficient mice produce large amounts of IL-31

Mon Jan 09 00:00:00 UTC 2017

An asthma-associated IL4R variant exacerbates airway inflammation by promoting conversion of regulatory T cells to TH17-like cells.

Nature medicine

Massoud AH,Charbonnier LM,Lopez D,Pellegrini M,Phipatanakul W,Chatila TA

14-0903 was used in Flow cytometry/Cell sorting to identify a previously unknown mechanism for the development of TH17 cell inflammation in genetically prone individuals.

Thu Sep 01 00:00:00 UTC 2016

Early ERK1/2 activation promotes DRP1-dependent mitochondrial fission necessary for cell reprogramming.

Nature communications

Prieto J,León M,Ponsoda X,Sendra R,Bort R,Ferrer-Lorente R,Raya A,López-García C,Torres J

Thu Mar 31 00:00:00 UTC 2016

Co-potentiation of antigen recognition: A mechanism to boost weak T cell responses and provide immunotherapy in vivo.

Science advances

Hoffmann MM,Molina-Mendiola C,Nelson AD,Parks CA,Reyes EE,Hansen MJ,Rajagopalan G,Pease LR,Schrum AG,Gil D

14-0903 was used in Flow cytometry/Cell sorting to investigate co-potentiation, showing that weak T-cell responses can be boosted to provide immunotherapy in vivo.

Thu Oct 01 00:00:00 UTC 2015

The B subunit of Escherichia coli heat-labile toxin alters the development and antigen-presenting capacity of dendritic cells.

Journal of cellular and molecular medicine

Ji J,Griffiths KL,Milburn PJ,Hirst TR,O'Neill HC

14-0903 was used in Magnetic cell separation to investigate the direct effect of intravenously administered EtxB on the size of the dendritic and myeloid cell populations in spleen.

Sat Aug 01 00:00:00 UTC 2015

Collaborative rewiring of the pluripotency network by chromatin and signalling modulating pathways.

Nature communications

Tran KA,Jackson SA,Olufs ZP,Zaidan NZ,Leng N,Kendziorski C,Roy S,Sridharan R

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 14-0903-82) in Flow Cytometry

Wed Feb 04 00:00:00 UTC 2015

Mechanisms of immunity in post-exposure vaccination against Ebola virus infection.

PloS one

Bradfute SB,Anthony SM,Stuthman KS,Ayithan N,Tailor P,Shaia CI,Bray M,Ozato K,Bavari S

14-0903 was used in Functional assays to analyse the expression levels of various cytokines in the serum of mice given an Ebola virus-like particle.

Tue Feb 23 00:00:00 UTC 2016

Innate immune cell CD45 regulates lymphopenia-induced T cell proliferation.

Journal of immunology (Baltimore, Md. : 1950)

Saunders AE,Shim YA,Johnson P

14-0903 was used in Flow cytometry/Cell sorting to demonstrate a novel role for CD45 on innate immune cells in promoting lymphopenia-induced T cell proliferation.

Mon Sep 15 00:00:00 UTC 2014

Recognition of tumor cells by Dectin-1 orchestrates innate immune cells for anti-tumor responses.

eLife

Chiba S,Ikushima H,Ueki H,Yanai H,Kimura Y,Hangai S,Nishio J,Negishi H,Tamura T,Saijo S,Iwakura Y,Taniguchi T

14-0903 was used in Flow cytometry/Cell sorting to show that recognition of tumor cells by Dectin-1 receptors leads to activation of the tumoricidal activity of NK cells.

Fri Aug 22 00:00:00 UTC 2014

Critical role of SAP in progression and reactivation but not maintenance of T cell-dependent humoral immunity.

Molecular and cellular biology

Zhong MC,Veillette A

14-0903 was used in Flow cytometry/Cell sorting to explore the therapeutic potential of blocking the SLAM-associated protein (SAP) for human autoimmune diseases.

Fri Mar 01 00:00:00 UTC 2013

Unexpected role for MHC II-peptide complexes in shaping CD8 T-cell expansion and differentiation in vivo.

Proceedings of the National Academy of Sciences of the United States of America

Do JS,Valujskikh A,Vignali DA,Fairchild RL,Min B

14-0903 was used in Flow cytometry/Cell sorting to reveal a previously undefined MHC II-dependent regulation that tunes CD8 T-cell reactivity.

Tue Jul 31 00:00:00 UTC 2012

Myelopoiesis in spleen-producing distinct dendritic-like cells.

Journal of cellular and molecular medicine

Tan JK,O'Neill HC

14-0903 was used in Magnetic cell separation to investigate whether the spleen represents a niche for development of myeloid dendritic-like cells.

Wed Aug 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; Thy-1 membrane glycoprotein

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Gene Aliases: CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2

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UniProt ID: (Mouse) P01831

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Entrez Gene ID: (Mouse) 21838

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Function(s)

GTPase activator activity integrin binding enzyme binding protein kinase binding GPI anchor binding

Process(es)

angiogenesis negative regulation of protein kinase activity cytoskeleton organization single organismal cell-cell adhesion negative regulation of cell migration positive regulation of GTPase activity retinal cone cell development protein autophosphorylation focal adhesion assembly negative regulation of axonogenesis T cell receptor signaling pathway negative regulation of T cell receptor signaling pathway positive regulation of T cell activation positive regulation of release of sequestered calcium ion into cytosol

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), eBioscience™

CD90.2 (Thy-1.2) Antibody (14-0903-82) in Flow

Product Details

14-0903-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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