Invitrogen

CD95 (APO-1/Fas) Monoclonal Antibody (SM 1-13), eBioscience™

View all (71) FAS (CD95) antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

BMS1030

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

Assay-Dependent
-

ELISA (ELISA)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

SM 1-13

Immunogen

Rec. Human CD95 (APO-1/Fas)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2-7.4

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10596849

Product Specific Information

Description: APO-1/Fas (CD95), a member of the TNF/NGF receptor superfamily, is a glycosylated 48 kDa surface protein containing a single transmembrane region. APO-1 is expressed on a variety of human B and T cell lines, on many different tumor cells and on various normal human tissues. APO-1 also occurs in a soluble form (sAPO-1) devoid of a transmembrane region. Triggering of APO-1 by its ligand or by certain anti-APO-1 monoclonal antibodies results in rapid induction of programmed cell death, apoptosis, in susceptible cells. The tissue distribution of APO-1 and of the APO-1 ligand suggests that the APO-1 receptor/ligand system plays an important role in various aspects of mammalian development and especially in the homeostasis of the immune system.

Expression of the APO-1 cell surface protein is enhanced by IFN-gamma and TNF and by activation in lymphocytes.

Applications Tested: ELISA, Flow Cytometry.

Target Information

CD95, also known as Fas or APO-1, is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor in the tumor necrosis factor receptor (TNFR) superfamily. It is expressed by a wide range of hematopoietic and non-hematopoietic cells, including monocytes, neutrophils, lymphocytes, and fibroblasts. CD95 also exists in a soluble form weighing approximately 26 kDa. The interaction of CD95 with its ligand, FasL, induces apoptosis, playing a crucial role in peripheral tolerance and anti-viral immune responses. Upon stimulation, CD95 leads to the aggregation of its intracellular Fas-associated death domains (FADD), formation of the death-inducing signaling complex (DISC), and activation of caspases. This cascade results in apoptosis. CD95 is also involved in activating NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and transduces proliferative signals in normal diploid fibroblasts and T cells. There are at least eight alternatively spliced transcript variants encoding seven distinct isoforms of CD95. Isoforms lacking the transmembrane domain may negatively regulate apoptosis mediated by the full-length isoform. The Fas/Fas ligand system is implicated in several human diseases, including AIDS, hepatitis, and cancer, highlighting its importance in immune regulation and disease pathology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; Apo1 antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; CD95; CD95 antigen; Fas (TNF receptor superfamily, member 6); Fas AMA; FASLG receptor; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; Tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6

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Gene Aliases: ALPS1A; APO-1; APT1; CD95; FAS; FAS1; FASTM; TNFRSF6

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UniProt ID: (Human) Q14293

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Entrez Gene ID: (Human) 355

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Function(s)
protease binding signal transducer activity receptor activity tumor necrosis factor-activated receptor activity protein binding kinase binding protein complex binding identical protein binding
Process(es)
ovarian follicle atresia immunoglobulin production renal system process protein complex assembly apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process activation-induced cell death of T cells inflammatory cell apoptotic process inflammatory response immune response signal transduction spermatogenesis brain development aging circadian rhythm extrinsic apoptotic signaling pathway via death domain receptors response to toxic substance gene expression regulation of gene expression B cell mediated immunity telencephalon development dendrite regeneration positive regulation of protein homooligomerization response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of cell proliferation response to drug ovulation cycle regulation of apoptotic process chordate embryonic development positive regulation of apoptotic process negative regulation of apoptotic process regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade response to peptide hormone negative thymic T cell selection regulation of lymphocyte differentiation regulation of myeloid cell differentiation response to cycloheximide spleen development negative regulation of B cell activation protein homooligomerization response to glucocorticoid maternal process involved in female pregnancy positive regulation of lymphocyte apoptotic process cellular response to hydrogen peroxide response to growth factor cellular response to phenylalanine cellular response to mechanical stimulus cellular response to cobalt ion cellular response to lithium ion cellular response to glucose stimulus cellular response to interleukin-1 cellular response to estrogen stimulus cellular response to hyperoxia cellular response to hypoxia cellular response to hydrostatic pressure motor neuron apoptotic process apoptotic signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand hepatocyte apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway liver regeneration necroptotic signaling pathway regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of extrinsic apoptotic signaling pathway via death domain receptors response to fluoride positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
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