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Alomone Labs, LTD
CD97/ADGRE5 (extracellular) Polyclonal Antibody
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FIGURE: 1 / 2
CD97/ADGRE5 (extracellular) Antibody (AER-055-200UL) in WB
Western blot analysis of HepG2 (lanes 1 and 5), Jurkat (lane 2 and 6), K562 (lanes 3 and 7) and THP-1 (lanes 4 and 8) cell lines lysates: - 1-4. Anti-Human CD97/ADGRE5 (extracellular) Antibody (#AER-055), (1:200). 5-8. Anti-Human CD97/ADGRE5 (extracellular) Antibody , preincubated with Human CD97/ADGRE5 (extracellular) Blocking Peptide (#BLP-ER055).
Product Details
AER-055-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)KSDSDRGGHWATEG, corresponding to amino acid residues 499-512 of human CD97/ADGRE5, Extracellular, N-terminus
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adhesion G protein-coupled receptor E5; CD97; CD97 molecule; Leukocyte antigen CD97; seven transmembrane helix receptor; seven-span transmembrane protein; seven-transmembrane, heterodimeric receptor associated with inflammation
Gene Aliases: ADGRE5; CD97; TM7LN1
UniProt ID: (Human) P48960
Entrez Gene ID: (Human) 976
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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