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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD99 Antibodies

          Invitrogen

          CD99 Monoclonal Antibody (M026)

          View all (64) CD99 antibodies

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          Cite CD99 Monoclonal Antibody (M026)

          • Antibody Testing Data (3)
          CD99 Antibody in Immunocytochemistry (ICC/IF)
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          CD99 Antibody in Immunocytochemistry (ICC/IF)
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          FIGURE: 1 / 3

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          CD99 Antibody (MA5-47656) in ICC/IF

          Immunocytochemistry analysis of CD99 in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse CD99 monoclonal antibody (Product # MA5-47656) (M026). The antibody was detected using goat anti-mouse DyLight® 594. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD99 Antibody in Immunocytochemistry (ICC/IF)
          CD99 Antibody in Western Blot (WB)
          CD99 Antibody in ELISA (ELISA)

          Product Details

          MA5-47656

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100
          -

          ELISA (ELISA)

          Assay-dependent
          -

          Immunoprecipitation (IP)

          1:100
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          M026

          Immunogen

          Clone M026 was generated from a proprietary antigen related to the extracellular region of human CD99 expressed in the MeWo melanoma cell line.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          ~0.5 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS with 1mg/mL BSA, 50% glycerol

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2942637

          Product Specific Information

          Clone M026 detects 30 and 32 kDa* bands corresponding to the molecular mass of the short and long forms of CD99 on SDS-PAGE immunoblots of human A431, LNCaP, MDA-MB-231, A549, and MeWo cell lysates, as well as mouse LL2 lung carcinoma. The antibody can be used for multiple applications including ELISA capture, western blot, immunocytochemistry, and immunoprecipitation. In addition, the antibody labels live, unfixed MeWo cells.

          Target Information

          CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: 12E7; antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13; CD99; CD99 antigen; cell surface antigen 12E7; cell surface antigen HBA-71; cell surface antigen O13; E2 antigen; MIC2 (monoclonal antibody 12E7); Paired immunoglobin-like type 2 receptor-ligand; PILR-L; Protein MIC2; surface antigen MIC2; T-cell surface glycoprotein E2

          View more View less

          Gene Aliases: 1110061M03Rik; 2410026K10Rik; CD99; D4; HBA71; MIC2; MIC2X; MIC2Y; MSK5X; pilr-1; Pilr-l; Pilrl

          View more View less

          UniProt ID: (Human) P14209, (Mouse) Q8VCN6

          View more View less

          Entrez Gene ID: (Human) 4267, (Rat) 652929, (Mouse) 673094

          View more View less

          Function(s)
          protein binding
          Process(es)
          cell adhesion myeloid dendritic cell activation
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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