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FIGURE: 1 / 1
CDC123 Antibody (PA5-88216) in WB
Western blot analysis of CDC123 in extracts of various cell lines. Samples were incubated with CDC123 Polyclonal antibody (Product # PA5-88216) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-88216
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human CDC123 (NP_0060142)
Conjugate
Form
Liquid
Concentration
1.06 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804750
Product Specific Information
Immunogen sequence: MKKEHVLHCQ FSAWYPFFRG VTIKSVILPL PQNVKDYLLD DGTLVVSGRD DPPTHSQPDS DDEAEEIQWS DDENTATLTA PEFPEFATKV QEAINSLGGS VFPKLNWSAP RDAYWIAMNS SLKCKTLSDI FLLFKSSDFI TRDFTQPFIH CTDDSPDPCI EYELVLRKWC ELIPGAEFRC FVKENKLIGI SQRDYTQYYD HISKQKEEIR RCIQDFFKKH IQYKFLDEDF VFDIYRDSRG KVWLIDFNPF GEVTDSLLFT WEELISENNL NGDFSEVDAQ EQDSPAFRCT NSEVTVQPSP YLSYRLPKDF VDLSTGEDAH KLIDFLKLKR NQQEDD; Positive Samples: HeLa, A431, HT-29, 293T, Mouse lung, Rat kidney; Cellular Location: Cytoplasm
Target Information
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid. The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase, and a homodimeric dihydrolipoamide dehydrogenase. E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell division cycle 123 homolog; Cell division cycle protein 123 homolog; D123 gene product; HT-1080; Protein D123; PZ32; Translation initiation factor eIF2 assembly protein
Gene Aliases: AA959893; C10orf7; CDC123; D123; G431001I09Rik
UniProt ID: (Human) O75794, (Rat) Q62834, (Mouse) Q8CII2
Entrez Gene ID: (Human) 8872, (Rat) 116656, (Mouse) 98828
cell division
cell cycle
formation of cytoplasmic translation initiation complex
regulation of translation
cell cycle arrest
regulation of mitotic cell cycle
positive regulation of cell proliferation
positive regulation of translational initiation
eukaryotic translation initiation factor 2 complex assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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