Proteintech

CDC25A Polyclonal Antibody

CDC25A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CDC25A Antibody (55031-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 55031-1-AP ( CDC25A Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

CDC25A Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

CDC25A Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

55031-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 25 publications 25 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.53 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M sodium bicarbonate/0.1M glycine, pH 7.3, with 50% glycerol, 0.1M glycine

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to CDC25A.

Target Information

CDC25a is a member of the CDC25 family of phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. CDC25a is required for progression through G1 and S phases of the cell cycle. CDC25a activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity.

Cell biology and toxicology

Luo Q,Pan Y,Fu Q,Zhang X,Zhou S,Yu P,Tian H,Liu P,Chen S,Zhang H,Qin T

Sun Oct 01 00:00:00 UTC 2023

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity.

Cell biology and toxicology

Luo Q,Pan Y,Fu Q,Zhang X,Zhou S,Yu P,Tian H,Liu P,Chen S,Zhang H,Qin T

Sun Oct 01 00:00:00 UTC 2023

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity.

Cell biology and toxicology

Luo Q,Pan Y,Fu Q,Zhang X,Zhou S,Yu P,Tian H,Liu P,Chen S,Zhang H,Qin T

Sun Oct 01 00:00:00 UTC 2023

ATM deficiency aggravates the progression of liver fibrosis induced by carbon tetrachloride in mice.

Toxicology

Li M,Yang Z,Song Z,Bo C,Wang S,Jia Q

Sun Jan 15 00:00:00 UTC 2023

Silibinin Therapy Improves Cholangiocarcinoma Outcomes by Regulating ERK/Mitochondrial Pathway.

Frontiers in pharmacology

Bai Y,Chen J,Hu W,Wang L,Wu Y,Yu S

Wed Apr 13 00:00:00 UTC 2022

Sulfate oligosaccharide of Gracilaria lemaneiformis modulates type 1 immunity by restraining T cell activation.

Carbohydrate polymers

Liu Q,Zhou Y,Ma L,Gu F,Liao K,Liu Y,Zhang Y,Liu H,Hong Y,Cao M,Liu WH,Liu C,Liu G

Fri Jul 15 00:00:00 UTC 2022

Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.

Nature communications

Xia J,Zhang J,Wu X,Du W,Zhu Y,Liu X,Liu Z,Meng B,Guo J,Yang Q,Wang Y,Wang Q,Feng X,Xie G,Shen Y,He Y,Xiang J,Wu M,An G,Qiu L,Jia W,Zhou W

Mon Jul 11 00:00:00 UTC 2022

Ubiquitin-Specific Protease 29 Regulates Cdc25A-Mediated Tumorigenesis.

International journal of molecular sciences

Chandrasekaran AP,Woo SH,Sarodaya N,Rhie BH,Tyagi A,Das S,Suresh B,Ko NR,Oh SJ,Kim KS,Ramakrishna S

Fri May 28 00:00:00 UTC 2021

Ubiquitin-Specific Protease 29 Regulates Cdc25A-Mediated Tumorigenesis.

International journal of molecular sciences

Chandrasekaran AP,Woo SH,Sarodaya N,Rhie BH,Tyagi A,Das S,Suresh B,Ko NR,Oh SJ,Kim KS,Ramakrishna S

Fri May 28 00:00:00 UTC 2021

BRD7 Promotes Cell Proliferation and Tumor Growth Through Stabilization of c-Myc in Colorectal Cancer.

Frontiers in cell and developmental biology

Zhao R,Liu Y,Wu C,Li M,Wei Y,Niu W,Yang J,Fan S,Xie Y,Li H,Wang W,Zeng Z,Xiong W,Li X,Li G,Zhou M

Sat Jun 12 00:00:00 UTC 2021

BRD7 Promotes Cell Proliferation and Tumor Growth Through Stabilization of c-Myc in Colorectal Cancer.

Frontiers in cell and developmental biology

Zhao R,Liu Y,Wu C,Li M,Wei Y,Niu W,Yang J,Fan S,Xie Y,Li H,Wang W,Zeng Z,Xiong W,Li X,Li G,Zhou M

Sat Jun 12 00:00:00 UTC 2021

YTHDF1 Promotes Gastric Carcinogenesis by Controlling Translation of FZD7.

Cancer research

Pi J,Wang W,Ji M,Wang X,Wei X,Jin J,Liu T,Qiang J,Qi Z,Li F,Liu Y,Ma Y,Si Y,Huo Y,Gao Y,Chen Y,Dong L,Su R,Chen J,Rao S,Yi P,Yu S,Wang F,Yu J

Sat May 15 00:00:00 UTC 2021

LINC00671 suppresses cell proliferation and metastasis in pancreatic cancer by inhibiting AKT and ERK signaling pathway.

Cancer gene therapy

Qu S,Niu K,Wang J,Dai J,Ganguly A,Gao C,Tian Y,Lin Z,Yang X,Zhang X,Liu Z,Li H

Thu Apr 01 00:00:00 UTC 2021

LINC00671 suppresses cell proliferation and metastasis in pancreatic cancer by inhibiting AKT and ERK signaling pathway.

Cancer gene therapy

Qu S,Niu K,Wang J,Dai J,Ganguly A,Gao C,Tian Y,Lin Z,Yang X,Zhang X,Liu Z,Li H

Thu Apr 01 00:00:00 UTC 2021

USP48 Governs Cell Cycle Progression by Regulating the Protein Level of Aurora B.

International journal of molecular sciences

Antao AM,Kaushal K,Das S,Singh V,Suresh B,Kim KS,Ramakrishna S

Sat Aug 07 00:00:00 UTC 2021

Hypericin-mediated photodynamic therapy inhibits growth of colorectal cancer cells via inducing S phase cell cycle arrest and apoptosis.

European journal of pharmacology

Hu J,Song J,Tang Z,Wei S,Chen L,Zhou R

Sat Jun 05 00:00:00 UTC 2021

6-Shogaol from ginger shows anti-tumor effect in cervical carcinoma via PI3K/Akt/mTOR pathway.

European journal of nutrition

Pei XD,He ZL,Yao HL,Xiao JS,Li L,Gu JZ,Shi PZ,Wang JH,Jiang LH

Sun Aug 01 00:00:00 UTC 2021

Cdc14a has a role in spermatogenesis, sperm maturation and male fertility.

Experimental cell research

Wen Z,Zhu H,Zhang A,Lin J,Zhang G,Liu D,Xiao Y,Ye C,Sun D,Wu B,Zhang J,Gao J

Thu Oct 01 00:00:00 UTC 2020

CLPTM1L induces estrogen receptor β signaling-mediated radioresistance in non-small cell lung cancer cells.

Cell communication and signaling : CCS

Li H,Che J,Jiang M,Cui M,Feng G,Dong J,Zhang S,Lu L,Liu W,Fan S

Thu Sep 17 00:00:00 UTC 2020

CLPTM1L induces estrogen receptor β signaling-mediated radioresistance in non-small cell lung cancer cells.

Cell communication and signaling : CCS

Li H,Che J,Jiang M,Cui M,Feng G,Dong J,Zhang S,Lu L,Liu W,Fan S

Thu Sep 17 00:00:00 UTC 2020

The REGγ inhibitor NIP30 increases sensitivity to chemotherapy in p53-deficient tumor cells.

Nature communications

Gao X,Wang Q,Wang Y,Liu J,Liu S,Liu J,Zhou X,Zhou L,Chen H,Pan L,Chen J,Wang D,Zhang Q,Shen S,Xiao Y,Wu Z,Cheng Y,Chen G,Kubra S,Qin J,Huang L,Zhang P,Wang C,Moses RE,Lonard DM,Malley BWO,Fares F,Zhang B,Li X,Li L,Xiao J

Thu Aug 06 00:00:00 UTC 2020

HAUSP stabilizes Cdc25A and protects cervical cancer cells from DNA damage response.

Biochimica et biophysica acta. Molecular cell research

Das S,Chandrasekaran AP,Jo KS,Ko NR,Oh SJ,Kim KS,Ramakrishna S

Tue Dec 01 00:00:00 UTC 2020

HAUSP stabilizes Cdc25A and protects cervical cancer cells from DNA damage response.

Biochimica et biophysica acta. Molecular cell research

Das S,Chandrasekaran AP,Jo KS,Ko NR,Oh SJ,Kim KS,Ramakrishna S

Tue Dec 01 00:00:00 UTC 2020

Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer.

Cell death and differentiation

Das S,Chandrasekaran AP,Suresh B,Haq S,Kang JH,Lee SJ,Kim J,Kim J,Lee S,Kim HH,Kim KS,Ramakrishna S

Sun Nov 01 00:00:00 UTC 2020

Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer.

Cell death and differentiation

Das S,Chandrasekaran AP,Suresh B,Haq S,Kang JH,Lee SJ,Kim J,Kim J,Lee S,Kim HH,Kim KS,Ramakrishna S

Sun Nov 01 00:00:00 UTC 2020

Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer.

Cell death and differentiation

Das S,Chandrasekaran AP,Suresh B,Haq S,Kang JH,Lee SJ,Kim J,Kim J,Lee S,Kim HH,Kim KS,Ramakrishna S

Sun Nov 01 00:00:00 UTC 2020

Cholesterol depletion sensitizes gallbladder cancer to cisplatin by impairing DNA damage response.

Cell cycle (Georgetown, Tex.)

Zhang Y,Liu Y,Duan J,Wang H,Zhang Y,Qiao K,Wang J

Sun Dec 01 00:00:00 UTC 2019

Decreased expression of miR-184 restrains the growth and invasion of endometrial carcinoma cells through CDC25A-dependent Notch signaling pathway.

American journal of translational research

Chen Z,Zhu Y,Fan X,Liu Y,Feng Q

Thu Oct 01 00:00:00 UTC 2020

RNA sequencing analysis of the CAL-27 cell response to over-expressed ZNF750 gene revealed an extensive regulation on cell cycle.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Liu X,Yang Y,Xu C,Yang H,Chen S,Chen H

Tue Oct 01 00:00:00 UTC 2019

A novel function of anaphase promoting complex subunit 10 in tumor progression in non-small cell lung cancer.

Cell cycle (Georgetown, Tex.)

Wang Y,Han T,Gan M,Guo M,Xie C,Jin J,Zhang S,Wang P,Cao J,Wang JB

Wed May 01 00:00:00 UTC 2019

PTBP3 splicing factor promotes hepatocellular carcinoma by destroying the splicing balance of NEAT1 and pre-miR-612.

Oncogene

Yang X,Qu S,Wang L,Zhang H,Yang Z,Wang J,Dai B,Tao K,Shang R,Liu Z,Li X,Zhang Z,Xia C,Ma B,Liu W,Li H,Dou K

Sat Dec 01 00:00:00 UTC 2018

PTBP3 splicing factor promotes hepatocellular carcinoma by destroying the splicing balance of NEAT1 and pre-miR-612.

Oncogene

Yang X,Qu S,Wang L,Zhang H,Yang Z,Wang J,Dai B,Tao K,Shang R,Liu Z,Li X,Zhang Z,Xia C,Ma B,Liu W,Li H,Dou K

Sat Dec 01 00:00:00 UTC 2018

Cell proliferation downregulated by TGF-β2-triggered G1/S checkpoint in clinical CAFs.

Cell cycle (Georgetown, Tex.)

Wu J,Fu R,Liu Z,Li G,Huang X,Xue Y,Xu Y,Sun Y,Zhao J,Mi J

Tue Jan 17 00:00:00 UTC 2017

Cell proliferation downregulated by TGF-β2-triggered G1/S checkpoint in clinical CAFs.

Cell cycle (Georgetown, Tex.)

Wu J,Fu R,Liu Z,Li G,Huang X,Xue Y,Xu Y,Sun Y,Zhao J,Mi J

Tue Jan 17 00:00:00 UTC 2017

A critical role of Hrd1 in the regulation of optineurin degradation and aggresome formation.

Human molecular genetics

Mao J,Xia Q,Liu C,Ying Z,Wang H,Wang G

Mon May 15 00:00:00 UTC 2017

2-Acetylamino-3-[4-(2-acetylamino-2-carboxyethylsulfanylcarbonylamino) phenyl carbamoylsulfanyl] propionic acid, a glutathione reductase inhibitor, induces G₂/M cell cycle arrest through generation of thiol oxidative stress in human esophageal cancer cells.

Oncotarget

Li X,Jiang Z,Feng J,Zhang X,Wu J,Chen W

Fri Sep 22 00:00:00 UTC 2017

Oncotarget

Li X,Jiang Z,Feng J,Zhang X,Wu J,Chen W

Fri Sep 22 00:00:00 UTC 2017

Inhibiting CREPT reduces the proliferation and migration of non-small cell lung cancer cells by down-regulating cell cycle related protein.

American journal of translational research

Liu T,Li WM,Wang WP,Sun Y,Ni YF,Xing H,Xia JH,Wang XJ,Zhang ZP,Li XF

Mon Jun 27 00:00:00 UTC 2016

Inhibiting CREPT reduces the proliferation and migration of non-small cell lung cancer cells by down-regulating cell cycle related protein.

American journal of translational research

Liu T,Li WM,Wang WP,Sun Y,Ni YF,Xing H,Xia JH,Wang XJ,Zhang ZP,Li XF

Mon Jun 27 00:00:00 UTC 2016

Inhibiting CREPT reduces the proliferation and migration of non-small cell lung cancer cells by down-regulating cell cycle related protein.

American journal of translational research

Liu T,Li WM,Wang WP,Sun Y,Ni YF,Xing H,Xia JH,Wang XJ,Zhang ZP,Li XF

Mon Jun 27 00:00:00 UTC 2016

Inhibiting CREPT reduces the proliferation and migration of non-small cell lung cancer cells by down-regulating cell cycle related protein.

American journal of translational research

Liu T,Li WM,Wang WP,Sun Y,Ni YF,Xing H,Xia JH,Wang XJ,Zhang ZP,Li XF

Mon Jun 27 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: CDC25 isoform A1-CAG; CDC25A2-CAG isoform; Dual specificity phosphatase Cdc25A; EC 3.1.3.48; M-phase inducer phosphatase 1; MPIP1

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Gene Aliases: CDC25A; CDC25A2; Cdc25m3; D9Ertd393e

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UniProt ID: (Human) P30304, (Mouse) P48964

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Entrez Gene ID: (Human) 993, (Mouse) 12530

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Function(s)

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding protein kinase binding hydrolase activity

Process(es)

regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle DNA replication mitotic nuclear division cell proliferation response to radiation cellular response to UV peptidyl-tyrosine dephosphorylation cell division regulation of cell cycle mitotic M phase protein dephosphorylation cell cycle dephosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CDC25A Polyclonal Antibody

CDC25A Antibody (55031-1-AP) in IHC (P)

Product Details

55031-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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