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CDC27 Antibody (A301-184A) in WB
Detection of human CDC27 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CDC27 antibody (Product # A301-184A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. CDC27 was also immunoprecipitated by rabbit anti-CDC27 antibody A301-183A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).
Product Details
A301-184A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 774 and 824 of human cell division cycle 27.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat and Bovine
Target Information
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AN CDC27Hs; anaphase promoting complex subunit 3; Anaphase-promoting complex subunit 3; anaphase-promoting complex, protein 3; APC3; CDC27 homolog; CDC27Hs; cell division cycle 27 homolog; Cell division cycle protein 27 homolog; H-NUC; nuc2 homolog
Gene Aliases: ANAPC3; APC3; CDC27; CDC27Hs; D0S1430E; D17S978E; H-NUC; HNUC; NUC2
UniProt ID: (Human) P30260
Entrez Gene ID: (Human) 996
metaphase/anaphase transition of mitotic cell cycle
cell proliferation
anaphase-promoting complex-dependent catabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
protein K11-linked ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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