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CDC7 Antibody (PA5-109501) in WB
Western blot analysis of CDC7 in extracts of various cell lines. Samples were incubated with CDC7 Polyclonal antibody (Product # PA5-109501) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-109501
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 275-574 of human CDC7 (NP_001127892.1).
Conjugate
Form
Liquid
Concentration
0.42 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2854912
Product Specific Information
Immunogen sequence: EGSVGLSVQR SVFGERNFNI HSSISHESPA VKLMKQSKTV DVLSRKLATK KKAISTKVMN SAVMRKTASS CPASLTCDCY ATDKVCSICL SRRQQVAPRA GTPGFRAPEV LTKCPNQTTA IDMWSAGVIF LSLLSGRYPF YKASDDLTAL AQIMTIRGSR ETIQAAKTFG KSILCSKEVP AQDLRKLCER LRGMDSSTPK LTSDIQGHAS HQPAISEKTD HKASCLVQTP PGQYSGNSFK KGDSNSCEHC FDEYNTNLEG WNEVPDEAYD LLDKLLDLNP ASRITAEEAL LHPFFKDMSL
Target Information
CDC7 is a cell division cycle 7 homolog protein that is critical for the G1/S transition and is also essential for initiation of DNA replication as cell division occurs. CDC7 is expressed in many normal tissues, but the overexpression of CDC7 may be associated with neoplastic transformation for some tumors and transformed cell lines. CDC7/DBF4 kinase promotes S phase by alleviating an inhibitory activity in Mcm4 that evolved to integrate several protein kinase. CDC7 encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1; Cdc7 Kinase; CDC7-like 1; CDC7-related kinase; cell division cycle 7 homolog; cell division cycle 7-like protein 1; Cell division cycle 7-related protein kinase; MGC117361; MGC126237; MGC126238; MGC91067
Gene Aliases: CDC7; CDC7L1; HsCDC7; Hsk1; huCDC7
UniProt ID: (Human) O00311
Entrez Gene ID: (Human) 8317
G1/S transition of mitotic cell cycle
double-strand break repair via break-induced replication
DNA replication
DNA replication initiation
phagocytosis
positive regulation of cell proliferation
regulation of cell shape
positive regulation of nuclear cell cycle DNA replication
positive regulation of G2/M transition of mitotic cell cycle
peptidyl-serine phosphorylation
cell cycle phase transition
cell division
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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