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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CDH11 Antibodies

          Invitrogen

          CDH11 Polyclonal Antibody

          Advanced Verification
          11 Published Figures
          27 References
          View all (37) CDH11 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite CDH11 Polyclonal Antibody

          • Antibody Testing Data (3)
          • Published Figures (11)
          • Advanced Verification (1)
          CDH11 Antibody in Immunocytochemistry (ICC/IF)
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          CDH11 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 15

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          CDH11 Antibody (71-7600) in ICC/IF

          Immunofluorescence analysis of CDH11/Cadherin OB was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CDH11/Cadherin OB Rabbit Polyclonal antibody (Product # 71-7600) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CDH11 Antibody in Immunocytochemistry (ICC/IF)
          CDH11 Antibody in Western Blot (WB)
          CDH11 Antibody in Western Blot (WB)
          CDH11 Antibody in Immunohistochemistry (IHC)
          CDH11 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          CDH11 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CDH11 Antibody in Immunohistochemistry (IHC)
          CDH11 Antibody in Immunohistochemistry (IHC)
          CDH11 Antibody in Immunohistochemistry (IHC)
          CDH11 Antibody in Immunohistochemistry (IHC)
          CDH11 Antibody in Western Blot (WB)
          CDH11 Antibody in Western Blot (WB)
          CDH11 Antibody in Western Blot (WB)
          CDH11 Antibody in Western Blot (WB)
          CDH11 Antibody

          Product Details

          71-7600

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          View 9 publications 9 publications

          Immunohistochemistry (IHC)

          Assay-dependent
          View 8 publications 8 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 3 publications 3 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          View 5 publications 5 publications

          ELISA (ELISA)

          Assay-dependent
          -

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Bovine, Chemical, Human, Mouse, Pig, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein derived from the intracellular domain of the human Cadherin 11 protein.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.25 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533995

          Target Information

          This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. All cadherins are single-pass transmembrane proteins with a variable number of 110 amino acid extracellular cadherin (EC) domains. Classical cadherins contain five large N-terminal EC domains, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Based on amino acid alignment, classical cadherins are divided into type I and type II subgroups. Type I cadherins, including cadherins E (epithelial), N (neural), P (placental), and R (retinal) cadherin differ from type I cadherins in their specific amino acid sequences. Type II cadherins include human cadherin-5, -6, -8, -11, and -12. Type II cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Cadherin-11 (OB-Cadherin) is a marker of the loosely connected and migratory cellular elements of the mesenchyme. Strong expression of Cadherin-11 has been noted in brain, spinal cord, bone marrow and bone cells, and is regulated in human endometrial glandular epithelial and stromal cells. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. A variant Cadherin-11 gene (variant form) encodes a truncated protein with an unusual cytoplasmic domain not present in other cadherins. The variant protein is generated by alternative splicing, utilizing a 179 bp intronic sequence. Recently, Cadherin-11 mRNA and protein were found to be expressed in most invasive breast cancer cell lines tested but not in non-invasive cell lines, suggesting that Cadherin-11 may be a marker for aggressive, invasive tumor subsets.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Cadherin; cadherin 11, type 2, OB-cadherin (osteoblast); Cadherin-11; OB Cadherin; OB-cadherin; OSF-4; Osteoblast cadherin; osteoblast-cadherin

          View more View less

          Gene Aliases: Cad-11; CAD11; CDH11; CDHOB; OB; OSF-4

          View more View less

          UniProt ID: (Human) P55287, (Mouse) P55288

          View more View less

          Entrez Gene ID: (Human) 1009, (Mouse) 12552

          View more View less

          Function(s)
          calcium ion binding metal ion binding
          Process(es)
          skeletal system development ossification cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules corticospinal tract morphogenesis adherens junction organization
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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