Invitrogen

CDK1 Monoclonal Antibody (POH-1)

1 Published Figure

6 References

View all (86) CDK1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (1)

CDK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

CDK1 Antibody (MA1-19057) in IHC (P)

Immunohistochemistry staining of human thymus (paraffin sections) using anti-Cdk1 (clone POH-1) Monoclonal antibody (Product # MA1-19057).

Product Image Gallery

Product Details

MA1-19057

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Bovine, Human, Non-human primate

Published species

Bovine, Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

POH-1

Immunogen

Bacterially expressed full-length human Cdk1 protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1075472

Product Specific Information

This antibody detects Cdk1 (p34Cdc2; an intracellular antigen). Staining of Cdk1 reflects the proliferating potential of respective tissue.

This antibody will not cross-react with Drosophila, mouse, Xenopus or rat.

Target Information

CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Combined Exogenous Activation of Bovine Oocytes: Effects on Maturation-Promoting Factor, Mitogen-Activated Protein Kinases, and Embryonic Competence.

International journal of molecular sciences

Valencia C,Pérez-García F,Aguila L,Felmer R,Arias ME

MA1-19057 was used in Western Blot to evaluate the effects of the combination of cycloheximide (CHX), dimethyl amino purine (DMAP), and anisomycin (ANY) on the activation of bovine oocytes, particularly on dynamics of MPF and MAPKs, embryonic developmental potential, and quality.

Tue Oct 31 00:00:00 UTC 2023

Combined Exogenous Activation of Bovine Oocytes: Effects on Maturation-Promoting Factor, Mitogen-Activated Protein Kinases, and Embryonic Competence.

International journal of molecular sciences

Valencia C,Pérez-García F,Aguila L,Felmer R,Arias ME

Tue Oct 31 00:00:00 UTC 2023

Biological dose estimation of UVA laser microirradiation utilizing charged particle-induced protein foci.

Mutagenesis

Splinter J,Jakob B,Lang M,Yano K,Engelhardt J,Hell SW,Chen DJ,Durante M,Taucher-Scholz G

MA1-19057 was used in immunocytochemistry to detect irradiation dose using charged particle-induced protein foci

Sat May 01 00:00:00 UTC 2010

Synthesis and structure-activity relationships of N-6 substituted analogues of 9-hydroxy-4-phenylpyrrolo[3,4-c]carbazole-1,3(2H,6H)-diones as inhibitors of Wee1 and Chk1 checkpoint kinases.

European journal of medicinal chemistry

Smaill JB,Baker EN,Booth RJ,Bridges AJ,Dickson JM,Dobrusin EM,Ivanovic I,Kraker AJ,Lee HH,Lunney EA,Ortwine DF,Palmer BD,Quin J,Squire CJ,Thompson AM,Denny WA

MA1-19057 was used in western blot to study the synthesis and SAR of novel small molecule inhibitors of the Wee1 and Chk1 checkpoint kinases

Sun Jun 01 00:00:00 UTC 2008

Hela /-CaD undergoes a DNA replication-associated switch in localization from the cytoplasm to the nuclei of endothelial cells/endothelial progenitor cells in human tumor vasculature.

Cancer biology & therapy

Zheng PP,Weiden Mv,Sillevis Smitt PA,Luider TM,Kros JM

MA1-19057 was used in western blot to examine the nuclear transport of caldsmon and its role in nuclear function

Fri Jun 01 00:00:00 UTC 2007

Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells.

The FEBS journal

Choi JK,Murillo G,Su BN,Pezzuto JM,Kinghorn AD,Mehta RG

MA1-19057 was used in western blot to study the antiproliferative and apoptotic effects of ixocarpalactone A on colon cancer cells

Fri Dec 01 00:00:00 UTC 2006

c-Myc alters the DNA damage-induced G2/M arrest in human mammary epithelial cells.

British journal of cancer

Sheen JH,Woo JK,Dickson RB

MA1-19057 was used in western blot to study the effect of c-Myc on DNA damage-induced G2/M arrest.

Mon Oct 20 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: ABL1; CDK1; cell cycle controller CDC2; Cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; Cell division protein kinase 1; Cyclin-dependent kinase 1; DKFZp686L20222; KCIP-1; MGC111195; MGC53829; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase; Protein 1054

View more View less

Gene Aliases: BOS_24079; CDC2; CDC28A; CDK1; CDKN1; P34CDC2

View more View less

UniProt ID: (Bovine) P48734, (Human) P06493

View more View less

Entrez Gene ID: (Bovine) 281061, (Human) 983

View more View less

Function(s)

cyclin-dependent protein serine/threonine kinase activity ATP binding RNA polymerase II carboxy-terminal domain kinase activity Hsp70 protein binding protein kinase activity protein serine/threonine kinase activity protein binding cyclin binding histone kinase activity

Process(es)

apoptotic process mitotic nuclear division mitotic G2 DNA damage checkpoint protein localization to kinetochore negative regulation of apoptotic process cell division G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle activation of MAPK activity microtubule cytoskeleton organization DNA replication DNA repair protein complex assembly DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic nuclear envelope disassembly centrosome cycle pronuclear fusion cell aging response to toxic substance positive regulation of gene expression regulation of Schwann cell differentiation response to organic cyclic compound response to amine response to activity cell migration histone phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation chromosome condensation epithelial cell differentiation organ regeneration anaphase-promoting complex-dependent catabolic process positive regulation of protein import into nucleus, translocation response to drug protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process response to ethanol positive regulation of DNA replication positive regulation of mitotic cell cycle regulation of embryonic development response to cadmium ion response to copper ion response to axon injury negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle ventricular cardiac muscle cell development positive regulation of cardiac muscle cell proliferation cellular response to hydrogen peroxide Golgi disassembly positive regulation of protein localization to nucleus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CDK1 Monoclonal Antibody (POH-1)

CDK1 Antibody (MA1-19057) in IHC (P)

Product Details

MA1-19057

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!