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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CDK2 Antibodies

          Proteintech

          CDK2 Polyclonal Antibody

          243 References
          View all (67) CDK2 antibodies
          Datasheet
          Protocols
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          Datasheet
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          Cite CDK2 Polyclonal Antibody

          • Antibody Testing Data (26)
          CDK2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          CDK2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 26

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          CDK2 Antibody (10122-1-AP) in ICC/IF

          Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2 antibody (Product # 10122-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CDK2 Antibody in Immunocytochemistry (ICC/IF)
          CDK2 Antibody in Immunocytochemistry (ICC/IF)
          CDK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CDK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Western Blot (WB)
          CDK2 Antibody in Flow Cytometry (Flow)
          CDK2 Antibody in Immunoprecipitation (IP)

          Product Details

          10122-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 221 publications 221 publications

          Immunohistochemistry (IHC)

          -
          View 11 publications 11 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200
          -

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          View 2 publications 2 publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cells
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 4 publications 4 publications

          Miscellaneous PubMed (MISC)

          -
          View 2 publications 2 publications

          Not applicable (N/A)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse, Zebrafish

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          CDK2 Fusion Protein Ag0166 (8-210 aa encoded by BC003065)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.4 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: EKIGEGTYG VVYKARNKLT GEVVALKKIR LDTETEGVPS TAIREISLLK ELNHPNIVKL LDVIHTENKL YLVFEFLHQD LKKFMDASAL TGIPLPLIKS YLFQLLQGLA FCHSHRVLHR DLKPQNLLIN TEGAIKLADF GLARAFGVPV RTYTHEVVTL WYRAPEILLG CKYYSTAVDI WSLGCIFAEM VTRRALFPGD SEID (8-210 aa encoded by BC003065)

          Target Information

          CDK2/cyclinO is a member of the cyclin dependent protein kinase family complexed to CyclinO. CyclinO was used as a specific protein isoform name for the UNG gene which is localized exclusively in the nucleus. In addition, CyclinO was reported to shows significant uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during G1 phase and the protein is turned over during the course of cell cycle. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: cdc2-related protein kinase; CDC28; CDC2A; CDK1; Cell division protein kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; EC 2.7.11.22; EC 2.7.11.23; kinase Cdc2; MPF; p33 protein kinase; p33CDK2; p34 protein kinase

          View more View less

          Gene Aliases: A630093N05Rik; CDK2; CDKN2; p33(CDK2)

          View more View less

          UniProt ID: (Human) P24941, (Mouse) P97377

          View more View less

          Entrez Gene ID: (Human) 1017, (Rat) 362817, (Mouse) 12566

          View more View less

          Function(s)
          protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding kinase activity cyclin binding protein complex binding histone kinase activity metal ion binding protein kinase activity nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups
          Process(es)
          G1/S transition of mitotic cell cycle lens development in camera-type eye DNA repair protein phosphorylation potassium ion transport mitotic nuclear division Ras protein signal transduction positive regulation of cell proliferation response to toxic substance histone phosphorylation peptidyl-serine phosphorylation organ regeneration positive regulation of DNA-dependent DNA replication initiation response to estradiol cellular response to insulin stimulus response to drug response to ethanol positive regulation of transcription, DNA-templated response to cadmium ion cell division meiotic cell cycle response to cAMP response to electrical stimulus regulation of cell cycle regulation of gene silencing cellular response to DNA damage stimulus cell cycle phosphorylation G2/M transition of mitotic cell cycle DNA replication DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest centriole replication meiotic nuclear division mitotic G1 DNA damage checkpoint centrosome duplication negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle cellular response to nitric oxide regulation of signal transduction by p53 class mediator
          It has to be done as per old AB suggested Products section.
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