OriGene

CDK2 Monoclonal Antibody (OTI1A8), TrueMAB™

CDK2 Antibody in Immunocytochemistry (ICC/IF)

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CDK2 Antibody (CF502943) in ICC/IF

Immunofluorescent staining of Hela cells using anti-CDK2 mouse monoclonal antibody (TA502943, green, upper left; merged, upper right) or Isotype control (merged, lower right). Cell nuclei were stained with DAPI (blue, lower left). (1:100)

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CDK2 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in CDK2 knockout (KO) 293T cell line using Anti-CDK2 Monoclonal Antibody (OTI1A8), TrueMAB™ (Product # CF502943). {KO}

Product Details

CF502943

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Immunocytochemistry (ICC/IF)

1:100

Flow Cytometry (Flow)

1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI1A8

Immunogen

Full length human recombinant protein of human CDK2 produced in HEK293T cell.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

CDK2/cyclinO is a member of the cyclin dependent protein kinase family complexed to CyclinO. CyclinO was used as a specific protein isoform name for the UNG gene which is localized exclusively in the nucleus. In addition, CyclinO was reported to shows significant uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during G1 phase and the protein is turned over during the course of cell cycle. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cdc2-related protein kinase; CDC28; CDC2A; CDK1; Cell division protein kinase 2; Cyclin-dependent kinase 2; EC 2.7.11.22; EC 2.7.11.23; kinase Cdc2; MPF; p33 protein kinase; p33CDK2; p34 protein kinase

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Gene Aliases: CDK2; CDKN2; p33(CDK2)

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UniProt ID: (Human) P24941

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Entrez Gene ID: (Human) 1017

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Function(s)

protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding kinase activity cyclin binding protein complex binding histone kinase activity metal ion binding

Process(es)

G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle lens development in camera-type eye DNA replication DNA repair potassium ion transport DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic nuclear division centriole replication meiotic nuclear division Ras protein signal transduction positive regulation of cell proliferation response to toxic substance histone phosphorylation peptidyl-serine phosphorylation organ regeneration mitotic G1 DNA damage checkpoint positive regulation of DNA-dependent DNA replication initiation response to estradiol cellular response to insulin stimulus response to drug response to ethanol positive regulation of transcription, DNA-templated response to cadmium ion centrosome duplication cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle response to cAMP response to electrical stimulus regulation of gene silencing cellular response to nitric oxide regulation of signal transduction by p53 class mediator

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CDK2 Monoclonal Antibody (OTI1A8), TrueMAB™

CDK2 Antibody (CF502943) in ICC/IF

Product Details

CF502943

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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