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Invitrogen
CDK2 Monoclonal Antibody (SP80)
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FIGURE: 1 / 9
CDK2 Antibody (MA5-16346) in ICC/IF
Immunofluorescence analysis of CDK2 was performed using 70% confluent log phase HCT 116 cells treated with Hydroxyurea (10mM for 18 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CDK2 Monoclonal Antibody (SP80) (Product # MA5-16346) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombina... View More
Product Details
MA5-16346
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
SP80
Immunogen
Synthetic peptide corresponding to C-terminus of human CDK2 protein.
Conjugate
Form
Liquid
Concentration
0.031 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.6, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2537865
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue (IHC) or Hela lysate (WB).
Target Information
CDK2/cyclinO is a member of the cyclin dependent protein kinase family complexed to CyclinO. CyclinO was used as a specific protein isoform name for the UNG gene which is localized exclusively in the nucleus. In addition, CyclinO was reported to shows significant uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during G1 phase and the protein is turned over during the course of cell cycle. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cdc2-related protein kinase; CDC28; CDC2A; CDK1; Cell division protein kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; EC 2.7.11.22; EC 2.7.11.23; kinase Cdc2; MPF; p33 protein kinase; p33CDK2; p34 protein kinase
Gene Aliases: A630093N05Rik; CDK2; CDKN2; p33(CDK2)
UniProt ID: (Human) P24941, (Mouse) P97377
Entrez Gene ID: (Human) 1017, (Rat) 362817, (Mouse) 12566
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
cyclin binding
protein complex binding
histone kinase activity
metal ion binding
protein kinase activity
nucleotide binding
transferase activity
transferase activity, transferring phosphorus-containing groups
G1/S transition of mitotic cell cycle
lens development in camera-type eye
DNA repair
protein phosphorylation
potassium ion transport
mitotic nuclear division
Ras protein signal transduction
positive regulation of cell proliferation
response to toxic substance
histone phosphorylation
peptidyl-serine phosphorylation
organ regeneration
positive regulation of DNA-dependent DNA replication initiation
response to estradiol
cellular response to insulin stimulus
response to drug
response to ethanol
positive regulation of transcription, DNA-templated
response to cadmium ion
cell division
meiotic cell cycle
response to cAMP
response to electrical stimulus
regulation of cell cycle
regulation of gene silencing
cellular response to DNA damage stimulus
cell cycle
phosphorylation
G2/M transition of mitotic cell cycle
DNA replication
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
centriole replication
meiotic nuclear division
mitotic G1 DNA damage checkpoint
centrosome duplication
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
cellular response to nitric oxide
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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