Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CDK4 Recombinant Rabbit Monoclonal Antibody (6N3M6)
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 8
CDK4 Antibody (MA5-35070) in IHC (P)
Immunohistochemistry analysis of CDK4 in paraffin-embedded human breast cancer using. Samples were incubated with CDK4 Monoclonal antibody (Product # MA5-35070) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
MA5-35070
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
6N3M6
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 204-303 of human CDK4 (NP_0000661)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848975
Product Specific Information
Immunogen sequence: FAEMFRRKPL FCGNSEADQL GKIFDLIGLP PEDDWPRDVS LPRGAFPPRG PRPVQSVVPE MEESGAQLLL EMLTFNPHKR ISAFRALQHS YLHKDEGNPE
Target Information
CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases:
Cell division protein kinase 4; CRK3; cyclin dependent kinase 4; Cyclin-dependent kinase 4; MGC14458; p34
Gene Aliases: CDK4; CMM3; Crk3; PSK-J3
UniProt ID: (Human) P11802, (Rat) P35426, (Mouse) P30285
Entrez Gene ID: (Human) 1019, (Rat) 94201, (Mouse) 12567
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
cyclin-dependent protein serine/threonine kinase regulator activity
cyclin binding
protein complex binding
protein kinase activity
kinase activity
nucleotide binding
protein serine/threonine kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
G1/S transition of mitotic cell cycle
lens development in camera-type eye
protein phosphorylation
signal transduction
circadian rhythm
positive regulation of cell proliferation
response to toxic substance
response to organic substance
response to lead ion
regulation of gene expression
positive regulation of G2/M transition of mitotic cell cycle
organ regeneration
response to testosterone
regulation of cell proliferation
response to drug
positive regulation of apoptotic process
positive regulation of translation
positive regulation of cell size
positive regulation of fibroblast proliferation
cell division
regulation of cell cycle
response to hyperoxia
negative regulation of cell cycle arrest
positive regulation of cell cycle
regulation of protein kinase activity
cell cycle
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support