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FIGURE: 1 / 1
CDK8 Antibody (MA5-55259) in WB
Western Blot analysis of various lysates using CDK8 Recombinant Monoclonal Antibody (Product # MA5-55259) at 1:2,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
MA5-55259
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2P3P8
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 364-464 of human CDK8.
Conjugate
Form
Liquid
Concentration
1.18 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667501
Product Specific Information
Immunogen Sequence: PDDKGDKKNQ QQQQGNNHTN GTGHPGNQDS SHTQGPPLKK VRVVPPTTTS GGLIMTSDYQ RSNPHAAYPN PGPSTSQPQS SMGYSATSQQ PPQYSHQTHR Y
Target Information
CDK8 phosphorylates the carboxy-terminal domain of the largest subunit of RNA polymerase II. This protein is coexpressed and copurified with Cyclin C. CDK8 is part of a family of proteins designated as cdks are critical regulators of cell cycle progression. The prototype member of this family, p34/Cdc 2, and a related protein Cdk 2, function late in the cycle while Cdk 4 and Cdk 6 are critically involved in G1 to S progression. Because of analogy with yeast Cdk 8, it has been proposed that the human Cdk 8 Cyclin C may interact with RNA polymerase, thus influencing the transcription mechanism(s) that lead to cell division.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDK8 protein kinase; Cell division protein kinase 8; Cyclin-dependent kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; MGC126074; MGC126075; Protein kinase K35
Gene Aliases: CDK8; K35
UniProt ID: (Human) P49336, (Mouse) Q8R3L8
Entrez Gene ID: (Human) 1024, (Mouse) 264064
protein kinase activity
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
RNA polymerase II carboxy-terminal domain kinase activity
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
ubiquitin protein ligase activity
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
phosphorylation
protein ubiquitination
negative regulation of triglyceride metabolic process
transcription initiation from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
regulation of cell cycle
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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