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Invitrogen
CDK9 Recombinant Rabbit Monoclonal Antibody (SD204-07)
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FIGURE: 1 / 12
CDK9 Antibody (MA5-32397) in ICC/IF
Immunofluorescence analysis of Cyclin-dependent kinase 9 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CDK9 Recombinant Rabbit Monoclonal Antibody (SD204-07) (Product # MA5-32397) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Second... View More
Product Details
MA5-32397
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SD204-07
Immunogen
Synthetic peptide within Human Cdk9 aa 323-372
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809675
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
CDK9 is a serine/threonine kinase which when complexed with CyclinT1 forms an active holoenzyme complex. This complex plays a key role in regulating the eukaryotic cell cycle. CDK9 is coexpressed and copurified with CyclinT1. CDK9 regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors. CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also has kinase activity towards CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy and confers a predisposition to heart failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-2K; CDC2-related kinase; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Cyclin-dependent kinase 9; RP11-228B15.5; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit
Gene Aliases: C-2k; CDC2L4; CDK9; CTK1; PITALRE; TAK
UniProt ID: (Human) P50750
Entrez Gene ID: (Human) 1025
RNA polymerase II core promoter sequence-specific DNA binding
transcription coactivator binding
DNA binding
chromatin binding
protein kinase activity
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
RNA polymerase II carboxy-terminal domain kinase activity
kinase activity
7SK snRNA binding
DNA repair
regulation of DNA repair
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
protein phosphorylation
regulation of mitotic cell cycle
cell proliferation
positive regulation of cardiac muscle hypertrophy
regulation of histone modification
replication fork processing
positive regulation of histone phosphorylation
response to drug
snRNA transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
positive regulation of viral transcription
regulation of muscle cell differentiation
negative regulation of cell cycle arrest
cellular response to cytokine stimulus
negative regulation of mRNA polyadenylation
positive regulation of mRNA 3'-UTR binding
positive regulation of histone H2B ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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