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Bethyl Laboratories
CEE Polyclonal Antibody
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FIGURE: 1 / 1
CEE Antibody (A302-613A) in WB
Detection of human CEE by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CEE antibody (Product # A302-613A) used for WB at 0.04 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 3 µg/mg lysate. CEE was also immunoprecipitated by rabbit anti-CEE antibody BL9430, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Product Details
A302-613A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 277 and 327 of human conserved edge expressed protein.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates in their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation (PubMed:20676083, PubMed:21636303, PubMed:21743475, PubMed:28104892). The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum (PubMed:20676083, PubMed:28104892, PubMed:25535373). Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome (PubMed:28104892). Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum (PubMed:21743475). The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome (PubMed:21636303). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: conserved edge expressed protein; conserved edge protein; Conserved edge-expressed protein; Golgi to ER traffic protein 4 homolog; H_NH1244M04.5; Transmembrane domain recognition complex 35 kDa subunit; transmembrane domain recognition complex, 35kDa; TRC35
Gene Aliases: C7orf20; CEE; CGI-20; GET4; TRC35
UniProt ID: (Human) Q7L5D6
Entrez Gene ID: (Human) 51608
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