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Bethyl Laboratories
CENP-F/Mitosin Polyclonal Antibody
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CENP-F/Mitosin Antibody (A301-611A) in ICC/IF
Detection of human CENP-F/Mitosin by immunocytochemistry. Samples: Acetone-fixed HeLa cells. Antibody: Affinity purified rabbit anti-CENP-F/Mitosin (Product # A301-611A) used at a dilution of 1:1,000 (1 µg/mL). Detection: Anti-rabbit IgG-FITC conjugated (A120-101F) used at a dilution of 1:100.
Product Details
A301-611A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1800 and 1850 of human centromere protein F (Mitosin).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For ICC, acetone fixation is recommended.
Target Information
This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitosis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AH antigen; cell-cycle-dependent 350K nuclear protein; CENP-F; CENP-F kinetochore protein; Centromere protein F; centromere protein F, 350/400ka (mitosin); centromere protein F, 350/400kDa (mitosin); Kinetochore protein CENPF; Mitosin; RP11-262H5.1
Gene Aliases: CENF; CENPF; CILD31; hcp-1; PRO1779; STROMS
UniProt ID: (Human) P49454
Entrez Gene ID: (Human) 1063
mitotic cell cycle
kidney development
chromosome segregation
sister chromatid cohesion
mitotic nuclear division
mitotic spindle assembly checkpoint
muscle organ development
cell proliferation
regulation of G2/M transition of mitotic cell cycle
protein transport
regulation of striated muscle tissue development
ventricular system development
cell differentiation
response to drug
negative regulation of transcription, DNA-templated
cell division
metaphase plate congression
kinetochore assembly
regulation of cell cycle
DNA biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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