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CENPA Antibody (PA5-95275) in WB
Western blot analysis of CENPA in Lane 1: rat liver tissue lysate (50 µg), Lane 2: rat lung tissue lysate (50 µg), Lane 3: rat pancreas tissue lysate (50 µg), Lane 4: HeLa whole cell lysate (40 µg). Samples were incubated with CENPA polyclonal antibody (Product # PA5-95275).
Product Details
PA5-95275
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human CENPA recombinant protein (Position: M1-G140).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807079
Product Specific Information
Human CENPA shares 71.6% amino acid (aa) sequence identity with mouse CENPA.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: Rat Liver Tissue, Rat Lung Tissue, Rat Pancreas Tissue, HELA whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CENP A; CENP-A; Centromere autoantigen A; Centromere protein A; centromere protein A, 17kDa; centromere-specific histone; Histone H3-like centromeric protein A
Gene Aliases: CenH3; CENP-A; CENPA; RGD1563607
UniProt ID: (Human) P49450
Entrez Gene ID: (Human) 1058, (Rat) 298850
Performance Guarantee
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