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Invitrogen
CENPB Polyclonal Antibody
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FIGURE: 1 / 1
CENPB Antibody (PA5-95842) in WB
Western blot analysis of CENPB in extracts of various cell lines. Samples were incubated with CENPB Polyclonal antibody (Product # PA5-95842) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5 min.
Product Details
PA5-95842
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human CENPB (NP_0018011)
Conjugate
Form
Liquid
Concentration
3.19 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2807644
Product Specific Information
Immunogen sequence: TLHFLEGGED SDSDSEEEDD EEEDDEDEDD DDDEEDGDEV PVPSFGEAMA YFAMVKRYLT SFPIDDRVQS HILHLEHDLV HVTRKNHARQ AGVRGLGHQS
Target Information
CENPB is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes and is considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CENP-B; centromere autoantigen B; Centromere protein B; Major centromere autoantigen B
Gene Aliases: Cenp-b; Cenpb
UniProt ID: (Mouse) P27790
Entrez Gene ID: (Rat) 362217, (Mouse) 12616
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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