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Invitrogen
CENPF Polyclonal Antibody
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CENPF Antibody (PA5-100716) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded CENPF in mouse kidney tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with CENPF polyclonal antibody (Product # PA5-100716) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-100716
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CENPF(Accession P49454), corresponding to amino acid residues 3072-3086.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2850220
Product Specific Information
Antibody detects endogenous levels of total CENPF.
Target Information
This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitosis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AH antigen; cell-cycle-dependent 350K nuclear protein; CENP-F; CENP-F kinetochore protein; centromere autoantigen F; Centromere protein F; centromere protein F, 350/400ka (mitosin); centromere protein F, 350/400kDa (mitosin); Kinetochore protein CENPF; leucine, glutamic acid, lysine family 1 protein; Mitosin; RP11-262H5.1
Gene Aliases: 6530404A22Rik; AI325968; CENF; CENPF; CILD31; hcp-1; Lek1; mitosin; PRO1779; STROMS
UniProt ID: (Human) P49454
Entrez Gene ID: (Human) 1063, (Mouse) 108000
mitotic cell cycle
chromosome segregation
regulation of G2/M transition of mitotic cell cycle
protein transport
regulation of striated muscle tissue development
negative regulation of transcription, DNA-templated
metaphase plate congression
kidney development
sister chromatid cohesion
mitotic nuclear division
mitotic spindle assembly checkpoint
muscle organ development
cell proliferation
ventricular system development
cell differentiation
response to drug
cell division
kinetochore assembly
regulation of cell cycle
DNA biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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