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Invitrogen
CENPF Polyclonal Antibody
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FIGURE: 1 / 3
CENPF Antibody (PA5-27126) in ICC/IF
Immunocytochemistry (Confocal immunofluorescence) of CENPF in paraformaldehyde-fixed HeLa. Samples were incubated with CENPF polyclonal antibody (Product # PA5-27126) using a dilution of 1:500 (green). Alpha-tubulin filaments were labeled with alpha Tubulin monoclonal antibody (Product # MA1-25054) (Red) at 1:2,500.
Product Details
PA5-27126
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CENPF. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2544602
Product Specific Information
Recommended positive controls: 293T, Raji.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitosis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AH antigen; cell-cycle-dependent 350K nuclear protein; CENP-F; CENP-F kinetochore protein; Centromere protein F; centromere protein F, (mitosin); centromere protein F, 350/400ka (mitosin); centromere protein F, 350/400kDa (mitosin); Kinetochore protein CENPF; Mitosin; RP11-262H5.1
Gene Aliases: CENF; CENPF; CILD31; hcp-1; im:7140452; im:7144238; im:7154719; im:7156761; PRO1779; si:ch211-152h14.2; STROMS
UniProt ID: (Human) P49454
Entrez Gene ID: (Human) 1063, (Zebrafish) 497403
kidney development
heart looping
ventricular system development
cilium morphogenesis
mitotic cell cycle
chromosome segregation
sister chromatid cohesion
mitotic nuclear division
mitotic spindle assembly checkpoint
muscle organ development
cell proliferation
regulation of G2/M transition of mitotic cell cycle
protein transport
regulation of striated muscle tissue development
cell differentiation
response to drug
negative regulation of transcription, DNA-templated
cell division
metaphase plate congression
kinetochore assembly
regulation of cell cycle
DNA biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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