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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CEP250 Antibodies

          Invitrogen

          CEP250 Polyclonal Antibody

          View all (9) CEP250 antibodies

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          Datasheet
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          Datasheet
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          Cite CEP250 Polyclonal Antibody

          • Antibody Testing Data (7)
          CEP250 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          CEP250 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          CEP250 Antibody (PA5-143966) in IHC (P)

          Immunohistochemistry analysis of CEP250 in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with CEP250 Polyclonal antibody (Product # PA5-143966) using a dilution of 2 μg/mL overnight at 4°C. Peroxidase Conjugated Goat Anti-rabb... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CEP250 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CEP250 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CEP250 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CEP250 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CEP250 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CEP250 Antibody in Western Blot (WB)
          CEP250 Antibody in Flow Cytometry (Flow)

          Product Details

          PA5-143966

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.25-0.5 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          2-5 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived human CEP250 recombinant protein (Position: Q563-K853).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3075180

          Product Specific Information

          Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

          Positive Control - WB: human Hela whole cell, human 293T whole cell, human HEL whole cell. IHC: human placenta tissue, human spleen tissue, human liver cancer tissue, mouse brain tissue, rat brain tissue. Flow: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          Probably plays an important role in centrosome cohesion during interphase.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: 250 kDa centrosomal protein; C-Nap1; Centrosomal Nek2-associated protein 1; Centrosomal protein 2; centrosomal protein 250; centrosomal protein 250kDa; centrosome associated protein; Centrosome-associated protein CEP250; Cep250; Intranuclear matrix protein

          View more View less

          Gene Aliases: AW490617; B230210E21Rik; C-NAP1; CEP2; CEP250; CNAP1; Inmp; RGD1562262

          View more View less

          UniProt ID: (Human) Q9BV73, (Mouse) Q60952

          View more View less

          Entrez Gene ID: (Human) 11190, (Rat) 311573, (Mouse) 16328

          View more View less

          Function(s)
          protein binding protein C-terminus binding protein kinase binding protein domain specific binding molecular_function
          Process(es)
          mitotic cell cycle protein localization biological_process centriole-centriole cohesion regulation of centriole-centriole cohesion protein localization to organelle cell cycle nonmotile primary cilium assembly G2/M transition of mitotic cell cycle
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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