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CEP290 Polyclonal Antibody

CEP290 Antibody in Immunocytochemistry (ICC/IF)

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CEP290 Antibody (22490-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 22490-1-AP (CEP290 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CEP290 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CEP290 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

22490-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CEP290 Fusion Protein Ag17457 (869-1103 aa encoded by BC008641)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.51 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Two isoforms were produced by alternative splicing with predicted MW of 290 and 180 kDa according to UniProt. 22490-1-AP can recognize both.

Immunogen sequence: QMDSDEMKK ILAENSRKIT VLQVNEKSLI RQYTTLVELE RQLRKENEKQ KNELLSMEAE VCEKIGCLQR FKEMAIFKIA ALQKVVDNSV SLSELELANK QYNELTAKYR DILQKDNMLV QRTSNLEHLE CENISLKEQV ESINKELEIT KEKLHTIEQA WEQETKLGNE SSMDKAKKSI TNSDIVSISK KITMLEMKEL NERQRAEHCQ KMYEHLRTSL KQMEERNFEL ETKFAEV (869-1103 aa encoded by BC008641)

Target Information

This gene encodes a protein with 13 putative coiled-coil domains, a region with homology to SMC chromosome segregation ATPases, six KID motifs, three tropomyosin homology domains and an ATP/GTP binding site motif A. The protein is localized to the centrosome and cilia and has sites for N-glycosylation, tyrosine sulfation, phosphorylation, N-myristoylation, and amidation. Mutations in this gene have been associated with Joubert syndrome and nephronophthisis and the presence of antibodies against this protein is associated with several forms of cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TTBK2 controls cilium stability by regulating distinct modules of centrosomal proteins.

Molecular biology of the cell

Nguyen A,Goetz SC

Sun Jan 01 00:00:00 UTC 2023

Phylogenetic profiling and cellular analyses of ARL16 reveal roles in traffic of IFT140 and INPP5E.

Molecular biology of the cell

Dewees SI,Vargová R,Hardin KR,Turn RE,Devi S,Linnert J,Wolfrum U,Caspary T,Eliáš M,Kahn RA

Fri Apr 01 00:00:00 UTC 2022

Centrosomal protein 290 is a novel prognostic indicator that modulates liver cancer cell ferroptosis via the Nrf2 pathway.

Aging

Shan Y,Yang G,Lu Q,Hu X,Qi D,Zhou Y,Xiao Y,Cao L,Tian F,Pan Q

Thu Mar 10 00:00:00 UTC 2022

Centrosomal protein 290 is a novel prognostic indicator that modulates liver cancer cell ferroptosis via the Nrf2 pathway.

Aging

Shan Y,Yang G,Lu Q,Hu X,Qi D,Zhou Y,Xiao Y,Cao L,Tian F,Pan Q

Thu Mar 10 00:00:00 UTC 2022

Centrosomal protein 290 is a novel prognostic indicator that modulates liver cancer cell ferroptosis via the Nrf2 pathway.

Aging

Shan Y,Yang G,Lu Q,Hu X,Qi D,Zhou Y,Xiao Y,Cao L,Tian F,Pan Q

Thu Mar 10 00:00:00 UTC 2022

The ARF GAPs ELMOD1 and ELMOD3 act at the Golgi and cilia to regulate ciliogenesis and ciliary protein traffic.

Molecular biology of the cell

Turn RE,Hu Y,Dewees SI,Devi N,East MP,Hardin KR,Khatib T,Linnert J,Wolfrum U,Lim MJ,Casanova JE,Caspary T,Kahn RA

Tue Feb 01 00:00:00 UTC 2022

The mechanism of acentrosomal spindle assembly in human oocytes.

Science (New York, N.Y.)

Wu T,Dong J,Fu J,Kuang Y,Chen B,Gu H,Luo Y,Gu R,Zhang M,Li W,Dong X,Sun X,Sang Q,Wang L

Fri Nov 18 00:00:00 UTC 2022

Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy.

EMBO reports

Martello A,Lauriola A,Mellis D,Parish E,Dawson JC,Imrie L,Vidmar M,Gammoh N,Mitić T,Brittan M,Mills NL,Carragher NO,D'Arca D,Caporali A

Fri Jul 03 00:00:00 UTC 2020

The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.

The Journal of biological chemistry

Datta P,Hendrickson B,Brendalen S,Ruffcorn A,Seo S

Fri Dec 13 00:00:00 UTC 2019

The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.

The Journal of biological chemistry

Datta P,Hendrickson B,Brendalen S,Ruffcorn A,Seo S

Fri Dec 13 00:00:00 UTC 2019

Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.

EMBO reports

Odabasi E,Gul S,Kavakli IH,Firat-Karalar EN

Sat Jun 01 00:00:00 UTC 2019

Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.

Scientific reports

Conkar D,Bayraktar H,Firat-Karalar EN

Thu Oct 03 00:00:00 UTC 2019

The small GTPase RSG1 controls a final step in primary cilia initiation.

The Journal of cell biology

Agbu SO,Liang Y,Liu A,Anderson KV

Tue Jan 02 00:00:00 UTC 2018

The small GTPase RSG1 controls a final step in primary cilia initiation.

The Journal of cell biology

Agbu SO,Liang Y,Liu A,Anderson KV

Tue Jan 02 00:00:00 UTC 2018

A CEP290 C-Terminal Domain Complements the Mutant CEP290 of Rd16 Mice In Trans and Rescues Retinal Degeneration.

Cell reports

Mookherjee S,Chen HY,Isgrig K,Yu W,Hiriyanna S,Levron R,Li T,Colosi P,Chien W,Swaroop A,Wu Z

Tue Oct 16 00:00:00 UTC 2018

A CEP290 C-Terminal Domain Complements the Mutant CEP290 of Rd16 Mice In Trans and Rescues Retinal Degeneration.

Cell reports

Mookherjee S,Chen HY,Isgrig K,Yu W,Hiriyanna S,Levron R,Li T,Colosi P,Chien W,Swaroop A,Wu Z

Tue Oct 16 00:00:00 UTC 2018

DAZ-interacting Protein 1 (Dzip1) Phosphorylation by Polo-like Kinase 1 (Plk1) Regulates the Centriolar Satellite Localization of the BBSome Protein during the Cell Cycle.

The Journal of biological chemistry

Zhang B,Wang G,Xu X,Yang S,Zhuang T,Wang G,Ren H,Cheng SY,Jiang Q,Zhang C

Fri Jan 27 00:00:00 UTC 2017

DAZ-interacting Protein 1 (Dzip1) Phosphorylation by Polo-like Kinase 1 (Plk1) Regulates the Centriolar Satellite Localization of the BBSome Protein during the Cell Cycle.

The Journal of biological chemistry

Zhang B,Wang G,Xu X,Yang S,Zhuang T,Wang G,Ren H,Cheng SY,Jiang Q,Zhang C

Fri Jan 27 00:00:00 UTC 2017

DAZ-interacting Protein 1 (Dzip1) Phosphorylation by Polo-like Kinase 1 (Plk1) Regulates the Centriolar Satellite Localization of the BBSome Protein during the Cell Cycle.

The Journal of biological chemistry

Zhang B,Wang G,Xu X,Yang S,Zhuang T,Wang G,Ren H,Cheng SY,Jiang Q,Zhang C

Fri Jan 27 00:00:00 UTC 2017

DAZ-interacting Protein 1 (Dzip1) Phosphorylation by Polo-like Kinase 1 (Plk1) Regulates the Centriolar Satellite Localization of the BBSome Protein during the Cell Cycle.

The Journal of biological chemistry

Zhang B,Wang G,Xu X,Yang S,Zhuang T,Wang G,Ren H,Cheng SY,Jiang Q,Zhang C

Fri Jan 27 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: Bardet-Biedl syndrome 14 protein; Bardet-Biedl syndrome 14 protein homolog; Cancer/testis antigen 87; centrosomal protein 290kDa; Centrosomal protein of 290 kDa; Cep290; CT87; CTCL tumor antigen se2-2; Meckel syndrome, type 4; monoclonal antibody 3H11 antigen; Nephrocystin-6; nephrocytsin-6; POC3 centriolar protein homolog; prostate cancer antigen T21; Tumor antigen se2-2

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Gene Aliases: 3H11Ag; b2b1454Clo; b2b1752Clo; BBS14; BC004690; CEP290; CT87; JBTS5; KIAA0373; LCA10; MKS4; NPHP6; POC3; rd16; RGD1311640; SLSN6

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UniProt ID: (Human) O15078, (Mouse) Q6A078

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Entrez Gene ID: (Human) 80184, (Mouse) 216274, (Rat) 314787

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Function(s)

protein binding identical protein binding microtubule minus-end binding molecular_function

Process(es)

G2/M transition of mitotic cell cycle protein transport hindbrain development otic vesicle formation cilium assembly eye photoreceptor cell development positive regulation of transcription, DNA-templated pronephros development cilium morphogenesis regulation of establishment of protein localization positive regulation of intracellular protein transport transcription, DNA-templated regulation of transcription, DNA-templated transport establishment or maintenance of cell polarity cell projection organization regulation of cAMP metabolic process nonmotile primary cilium assembly photoreceptor cell maintenance retina development in camera-type eye biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CEP290 Polyclonal Antibody

CEP290 Antibody (22490-1-AP) in ICC/IF

Product Details

22490-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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