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CEP55 Antibody (PA5-29454) in ICC/IF
Detection of differential subcellular localization of CEP55 protein during different phases of mitosis demonstrates antibody specificity. Immunofluorescence analysis using Anti-CEP55 Polyclonal Antibody (Product # PA5-29454), shows its expression at different phases of mitosis in NTERA cells where it is associated with the centrosome throughout the mitotic division. It can be seen at high levels in the condensing chromosomes during Prophase; concentrated near the spindle poles during Metaphase, starts separating in the spindle midzone at A... View More
Product Details
PA5-29454
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human CEP55. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.85 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2546930
Product Specific Information
Recommended positive controls: IMR32, U87-MG, MCF-7, HeLa.
Predicted reactivity: Rat (80%), Bovine (87%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Plays a role in mitotic exit and cytokinesis (PubMed:16198290, PubMed:17853893). Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis (PubMed:17853893). Not required for microtubule nucleation (PubMed:16198290). Plays a role in the development of the brain and kidney (PubMed:28264986). [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cancer/testis antigen 111; centrosomal protein 55kDa; Centrosomal protein of 55 kDa; Cep55; Up-regulated in colon cancer 6
Gene Aliases: C10orf3; CEP55; CT111; URCC6
UniProt ID: (Human) Q53EZ4
Entrez Gene ID: (Human) 55165
Performance Guarantee
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