Proteintech

CEP63 Polyclonal Antibody

CEP63 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

CEP63 Antibody (16268-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CEP63 antibody (Product # 16268-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CEP63 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CEP63 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16268-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 9 publications 9 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CEP63 Fusion Protein Ag9335 (1-349 aa encoded by BC014050)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.59 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).

Immunogen sequence: MEALLEGIQ NRGHGGGFLT SCEAELQELM KQIDIMVAHK KSEWEGRTHA LETCLKIREQ ELKSLRSQLD VTHKEVGMLH QQVEEHEKIK QEMTMEYKQE LKKLHEELCI LKRSYEKLQK KQMREFRGNT KNHREDRSEI ERLTAKIEEF RQKSLDWEKQ RLIYQQQVSS LEAQRKALAE QSEIIQAQLV NRKQKLESVE LSSQSEIQHL SSKLERANDT ICANELEIER LTMRVNDLVG TSMTVLQEQQ QKEEKLRESE KLLEALQEEK RELKAALQSQ ENLIHEARIQ KEKLQEKVKA TNTQHAVEAI SLESVSATCK QLSQELMEKY EELKRMEAHN NEYKAEIKKL (1-349 aa encoded by BC014050)

Target Information

CEP63 is a protein with six coiled-coil domains. The protein is localized to the centrosome, a non-membranous organelle that functions as the major microtubule-organizing center in animal cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Zika virus alters centrosome organization to suppress the innate immune response.

EMBO reports

Kodani A,Knopp KA,Di Lullo E,Retallack H,Kriegstein AR,DeRisi JL,Reiter JF

Mon Sep 05 00:00:00 UTC 2022

CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72.

BMC biology

Ying Z,Wang K,Wu J,Wang M,Yang J,Wang X,Zhou G,Chen H,Xu H,Sze SCW,Gao F,Li C,Sha O

Mon Oct 24 00:00:00 UTC 2022

CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72.

BMC biology

Ying Z,Wang K,Wu J,Wang M,Yang J,Wang X,Zhou G,Chen H,Xu H,Sze SCW,Gao F,Li C,Sha O

Mon Oct 24 00:00:00 UTC 2022

The mechanism of acentrosomal spindle assembly in human oocytes.

Science (New York, N.Y.)

Wu T,Dong J,Fu J,Kuang Y,Chen B,Gu H,Luo Y,Gu R,Zhang M,Li W,Dong X,Sun X,Sang Q,Wang L

Fri Nov 18 00:00:00 UTC 2022

Cep57 and Cep57L1 maintain centriole engagement in interphase to ensure centriole duplication cycle.

The Journal of cell biology

Ito KK,Watanabe K,Ishida H,Matsuhashi K,Chinen T,Hata S,Kitagawa D

Mon Mar 01 00:00:00 UTC 2021

Massive centriole production can occur in the absence of deuterosomes in multiciliated cells.

Nature cell biology

Mercey O,Levine MS,LoMastro GM,Rostaing P,Brotslaw E,Gomez V,Kumar A,Spassky N,Mitchell BJ,Meunier A,Holland AJ

Sun Dec 01 00:00:00 UTC 2019

Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites.

Nature communications

Holdgaard SG,Cianfanelli V,Pupo E,Lambrughi M,Lubas M,Nielsen JC,Eibes S,Maiani E,Harder LM,Wesch N,Foged MM,Maeda K,Nazio F,de la Ballina LR,Dötsch V,Brech A,Frankel LB,Jäättelä M,Locatelli F,Barisic M,Andersen JS,Bekker-Jensen S,Lund AH,Rogov VV,Papaleo E,Lanzetti L,De Zio D,Cecconi F

Fri Sep 13 00:00:00 UTC 2019

Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.

Nature communications

Mori M,Hazan R,Danielian PS,Mahoney JE,Li H,Lu J,Miller ES,Zhu X,Lees JA,Cardoso WV

Tue Jul 04 00:00:00 UTC 2017

Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.

Nature communications

Mori M,Hazan R,Danielian PS,Mahoney JE,Li H,Lu J,Miller ES,Zhu X,Lees JA,Cardoso WV

Tue Jul 04 00:00:00 UTC 2017

Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.

Neuron

Jayaraman D,Kodani A,Gonzalez DM,Mancias JD,Mochida GH,Vagnoni C,Johnson J,Krogan N,Harper JW,Reiter JF,Yu TW,Bae BI,Walsh CA

Wed Nov 23 00:00:00 UTC 2016

Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.

Neuron

Jayaraman D,Kodani A,Gonzalez DM,Mancias JD,Mochida GH,Vagnoni C,Johnson J,Krogan N,Harper JW,Reiter JF,Yu TW,Bae BI,Walsh CA

Wed Nov 23 00:00:00 UTC 2016

Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.

Neuron

Jayaraman D,Kodani A,Gonzalez DM,Mancias JD,Mochida GH,Vagnoni C,Johnson J,Krogan N,Harper JW,Reiter JF,Yu TW,Bae BI,Walsh CA

Wed Nov 23 00:00:00 UTC 2016

Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.

Neuron

Jayaraman D,Kodani A,Gonzalez DM,Mancias JD,Mochida GH,Vagnoni C,Johnson J,Krogan N,Harper JW,Reiter JF,Yu TW,Bae BI,Walsh CA

Wed Nov 23 00:00:00 UTC 2016

Autophagy controls centrosome number by degrading Cep63.

Nature communications

Watanabe Y,Honda S,Konishi A,Arakawa S,Murohashi M,Yamaguchi H,Torii S,Tanabe M,Tanaka S,Warabi E,Shimizu S

Mon Nov 21 00:00:00 UTC 2016

Autophagy controls centrosome number by degrading Cep63.

Nature communications

Watanabe Y,Honda S,Konishi A,Arakawa S,Murohashi M,Yamaguchi H,Torii S,Tanabe M,Tanaka S,Warabi E,Shimizu S

Mon Nov 21 00:00:00 UTC 2016

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.

eLife

Kodani A,Yu TW,Johnson JR,Jayaraman D,Johnson TL,Al-Gazali L,Sztriha L,Partlow JN,Kim H,Krup AL,Dammermann A,Krogan NJ,Walsh CA,Reiter JF

Sat Aug 22 00:00:00 UTC 2015

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.

eLife

Kodani A,Yu TW,Johnson JR,Jayaraman D,Johnson TL,Al-Gazali L,Sztriha L,Partlow JN,Kim H,Krup AL,Dammermann A,Krogan NJ,Walsh CA,Reiter JF

Sat Aug 22 00:00:00 UTC 2015

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.

eLife

Kodani A,Yu TW,Johnson JR,Jayaraman D,Johnson TL,Al-Gazali L,Sztriha L,Partlow JN,Kim H,Krup AL,Dammermann A,Krogan NJ,Walsh CA,Reiter JF

Sat Aug 22 00:00:00 UTC 2015

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.

eLife

Kodani A,Yu TW,Johnson JR,Jayaraman D,Johnson TL,Al-Gazali L,Sztriha L,Partlow JN,Kim H,Krup AL,Dammermann A,Krogan NJ,Walsh CA,Reiter JF

Sat Aug 22 00:00:00 UTC 2015

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.

eLife

Kodani A,Yu TW,Johnson JR,Jayaraman D,Johnson TL,Al-Gazali L,Sztriha L,Partlow JN,Kim H,Krup AL,Dammermann A,Krogan NJ,Walsh CA,Reiter JF

Sat Aug 22 00:00:00 UTC 2015

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.

eLife

Kodani A,Yu TW,Johnson JR,Jayaraman D,Johnson TL,Al-Gazali L,Sztriha L,Partlow JN,Kim H,Krup AL,Dammermann A,Krogan NJ,Walsh CA,Reiter JF

Sat Aug 22 00:00:00 UTC 2015

Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers.

Nature communications

He S,Zhao Z,Yang Y,O'Connell D,Zhang X,Oh S,Ma B,Lee JH,Zhang T,Varghese B,Yip J,Dolatshahi Pirooz S,Li M,Zhang Y,Li GM,Ellen Martin S,Machida K,Liang C

Mon Aug 03 00:00:00 UTC 2015

Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers.

Nature communications

He S,Zhao Z,Yang Y,O'Connell D,Zhang X,Oh S,Ma B,Lee JH,Zhang T,Varghese B,Yip J,Dolatshahi Pirooz S,Li M,Zhang Y,Li GM,Ellen Martin S,Machida K,Liang C

Mon Aug 03 00:00:00 UTC 2015

Cep63 and cep152 cooperate to ensure centriole duplication.

PloS one

Brown NJ,Marjanović M,Lüders J,Stracker TH,Costanzo V

Tue Apr 01 00:00:00 UTC 2014

Cep63 and cep152 cooperate to ensure centriole duplication.

PloS one

Brown NJ,Marjanović M,Lüders J,Stracker TH,Costanzo V

Tue Apr 01 00:00:00 UTC 2014

A dual role for UVRAG in maintaining chromosomal stability independent of autophagy.

Developmental cell