Proteintech

CEP97 Polyclonal Antibody

CEP97 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

CEP97 Antibody (22050-1-AP) in ICC/IF

Immunofluorescent analysis of MDCK cells using 22050-1-AP (CEP97 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CEP97 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CEP97 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

22050-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 11 publications 11 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CEP97 Fusion Protein Ag16995 (506-865 aa encoded by BC041085)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.

Immunogen sequence: ESTEQKQSD IKKPENTQPE NKETISQATS EKLPMILTQR SVALGQDKVA LQKLNDAATK LQACWRGFYA RNYNPQAKDV RYEIRLRRMQ EHIVCLTDEI RRLRKERDEE RIKKFVQEEA FRFLWNQVRS LQVWQQTVDQ RLSSWHTDVP PISSTLVPSK HPLFTQSQES SCDQNADWFI ASDVAPQEKS LPEFPDSGFH SSLTEQVHSL QHSLDFEKSS TEGSESSIMG NSIDTVRYGK ESDLGDVSEE HGEWNKESSN NEQDNSLLEQ YLTSVQQLED ADERTNFDTE TRDSKLHIAC FPVQLDTLSD GASVDESHGI SPPLQGEISQ TQENSKLNAE VQGQQPECDS TFQLLHVGVT V (506-865 aa encoded by BC041085)

Target Information

CEP97 (centrosomal protein of 97 kDa) has been identified as part of a complex with CP110. Together, CP110 and CEP97 have been shown to play a role in the assembly of cilia. Loss of CEP97 or CEP110 promotes cilia formation while forced expression inhibits ciliogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis.

eLife

Hall EA,Kumar D,Prosser SL,Yeyati PL,Herranz-Pérez V,García-Verdugo JM,Rose L,McKie L,Dodd DO,Tennant PA,Megaw R,Murphy LC,Ferreira MF,Grimes G,Williams L,Quidwai T,Pelletier L,Reiter JF,Mill P

Wed Feb 15 00:00:00 UTC 2023

Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis.

eLife

Hall EA,Kumar D,Prosser SL,Yeyati PL,Herranz-Pérez V,García-Verdugo JM,Rose L,McKie L,Dodd DO,Tennant PA,Megaw R,Murphy LC,Ferreira MF,Grimes G,Williams L,Quidwai T,Pelletier L,Reiter JF,Mill P

Wed Feb 15 00:00:00 UTC 2023

CCP5 and CCP6 retain CP110 and negatively regulate ciliogenesis.

BMC biology

Wang Y,Zhang Y,Guo X,Zheng Y,Zhang X,Feng S,Wu HY

Wed May 24 00:00:00 UTC 2023

LUBAC regulates ciliogenesis by promoting CP110 removal from the mother centriole.

The Journal of cell biology

Shen XL,Yuan JF,Qin XH,Song GP,Hu HB,Tu HQ,Song ZQ,Li PY,Xu YL,Li S,Jian XX,Li JN,He CY,Yu XP,Liang LY,Wu M,Han QY,Wang K,Li AL,Zhou T,Zhang YC,Wang N,Li HY

Mon Jan 03 00:00:00 UTC 2022

LUBAC regulates ciliogenesis by promoting CP110 removal from the mother centriole.

The Journal of cell biology

Shen XL,Yuan JF,Qin XH,Song GP,Hu HB,Tu HQ,Song ZQ,Li PY,Xu YL,Li S,Jian XX,Li JN,He CY,Yu XP,Liang LY,Wu M,Han QY,Wang K,Li AL,Zhou T,Zhang YC,Wang N,Li HY

Mon Jan 03 00:00:00 UTC 2022

ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.

EMBO reports

Song T,Yang Y,Zhou P,Ran J,Zhang L,Wu X,Xie W,Zhong T,Liu H,Liu M,Li D,Zhao H,Zhou J

Wed May 04 00:00:00 UTC 2022

ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.

EMBO reports

Song T,Yang Y,Zhou P,Ran J,Zhang L,Wu X,Xie W,Zhong T,Liu H,Liu M,Li D,Zhao H,Zhou J

Wed May 04 00:00:00 UTC 2022

A ciliopathy complex builds distal appendages to initiate ciliogenesis.

The Journal of cell biology

Kumar D,Rains A,Herranz-Pérez V,Lu Q,Shi X,Swaney DL,Stevenson E,Krogan NJ,Huang B,Westlake C,Garcia-Verdugo JM,Yoder BK,Reiter JF

Mon Sep 06 00:00:00 UTC 2021

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Cell research

Liu M,Zhang W,Li M,Feng J,Kuang W,Chen X,Yang F,Sun Q,Xu Z,Hua J,Yang C,Liu W,Shu Q,Yang Y,Zhou T,Xie S

Mon Nov 01 00:00:00 UTC 2021

LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome.

eLife

Bozal-Basterra L,Gonzalez-Santamarta M,Muratore V,Bermejo-Arteagabeitia A,Da Fonseca C,Barroso-Gomila O,Azkargorta M,Iloro I,Pampliega O,Andrade R,Martín-Martín N,Branon TC,Ting AY,Rodríguez JA,Carracedo A,Elortza F,Sutherland JD,Barrio R

Thu Jun 18 00:00:00 UTC 2020

LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome.

eLife

Thu Jun 18 00:00:00 UTC 2020

Cep97 Is Required for Centriole Structural Integrity and Cilia Formation in Drosophila.

Current biology : CB

Dobbelaere J,Schmidt Cernohorska M,Huranova M,Slade D,Dammermann A

Mon Aug 03 00:00:00 UTC 2020

Cep97 Is Required for Centriole Structural Integrity and Cilia Formation in Drosophila.

Current biology : CB

Dobbelaere J,Schmidt Cernohorska M,Huranova M,Slade D,Dammermann A

Mon Aug 03 00:00:00 UTC 2020

Altered gene regulation as a candidate mechanism by which ciliopathy gene SDCCAG8 contributes to schizophrenia and cognitive function.

Human molecular genetics

Flynn M,Whitton L,Donohoe G,Morrison CG,Morris DW

Sat Feb 01 00:00:00 UTC 2020

Phosphorylation of CEP83 by TTBK2 is necessary for cilia initiation.

The Journal of cell biology

Lo CH,Lin IH,Yang TT,Huang YC,Tanos BE,Chou PC,Chang CW,Tsay YG,Liao JC,Wang WJ

Mon Oct 07 00:00:00 UTC 2019

A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.

Cell reports

Frikstad KM,Molinari E,Thoresen M,Ramsbottom SA,Hughes F,Letteboer SJF,Gilani S,Schink KO,Stokke T,Geimer S,Pedersen LB,Giles RH,Akhmanova A,Roepman R,Sayer JA,Patzke S

Tue Aug 13 00:00:00 UTC 2019

CCDC84 Acetylation Oscillation Regulates Centrosome Duplication by Modulating HsSAS-6 Degradation.

Cell reports

Wang T,Zou Y,Huang N,Teng J,Chen J

Tue Nov 12 00:00:00 UTC 2019

Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1.

Protein & cell

Mao H,Tang Z,Li H,Sun B,Tan M,Fan S,Zhu Y,Sun Y

Tue Oct 01 00:00:00 UTC 2019

Truncated SALL1 Impedes Primary Cilia Function in Townes-Brocks Syndrome.

American journal of human genetics

Bozal-Basterra L,Martín-Ruíz I,Pirone L,Liang Y,Sigurðsson JO,Gonzalez-Santamarta M,Giordano I,Gabicagogeascoa E,de Luca A,Rodríguez JA,Wilkie AOM,Kohlhase J,Eastwood D,Yale C,Olsen JV,Rauchman M,Anderson KV,Sutherland JD,Barrio R

Thu Feb 01 00:00:00 UTC 2018

Truncated SALL1 Impedes Primary Cilia Function in Townes-Brocks Syndrome.

American journal of human genetics

Thu Feb 01 00:00:00 UTC 2018

Truncated SALL1 Impedes Primary Cilia Function in Townes-Brocks Syndrome.

American journal of human genetics

Thu Feb 01 00:00:00 UTC 2018

Truncated SALL1 Impedes Primary Cilia Function in Townes-Brocks Syndrome.

American journal of human genetics

Thu Feb 01 00:00:00 UTC 2018

M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.

Nature communications

Huang N,Zhang D,Li F,Chai P,Wang S,Teng J,Chen J

Tue Oct 30 00:00:00 UTC 2018

M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.

Nature communications

Huang N,Zhang D,Li F,Chai P,Wang S,Teng J,Chen J

Tue Oct 30 00:00:00 UTC 2018

M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.

Nature communications

Huang N,Zhang D,Li F,Chai P,Wang S,Teng J,Chen J

Tue Oct 30 00:00:00 UTC 2018

M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.

Nature communications

Huang N,Zhang D,Li F,Chai P,Wang S,Teng J,Chen J

Tue Oct 30 00:00:00 UTC 2018

Centrin2 regulates CP110 removal in primary cilium formation.

The Journal of cell biology

Prosser SL,Morrison CG

Mon Mar 16 00:00:00 UTC 2015

Centrin2 regulates CP110 removal in primary cilium formation.

The Journal of cell biology

Prosser SL,Morrison CG

Mon Mar 16 00:00:00 UTC 2015

Mother Centriole Distal Appendages Mediate Centrosome Docking at the Immunological Synapse and Reveal Mechanistic Parallels with Ciliogenesis.

Current biology : CB

Stinchcombe JC,Randzavola LO,Angus KL,Mantell JM,Verkade P,Griffiths GM

Mon Dec 21 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: centrosomal protein 97kDa; Centrosomal protein of 97 kDa; Cep97; Leucine-rich repeat and IQ domain-containing protein 2; leucine-rich repeats and IQ motif containing 2

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Gene Aliases: 2810403B08Rik; CEP97; LRRIQ2

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UniProt ID: (Human) Q8IW35

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Entrez Gene ID: (Dog) 608540, (Human) 79598

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Function(s)

protein binding calmodulin binding

Process(es)

cell projection organization regulation of mitotic spindle assembly negative regulation of cilium assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CEP97 Polyclonal Antibody

CEP97 Antibody (22050-1-AP) in ICC/IF

Product Details

22050-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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