Invitrogen

CER1 Monoclonal Antibody (9D6)

CER1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

CER1 Antibody (MA5-15554) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human gastric cancer (left) and normal gastric tissues (right) using CER1 monoclonal antibody (Product # MA5-15554) followed with DAB staining.

Product Image Gallery

CER1 Antibody — Fig. 4 MEG induces nearly homogeneous definitive endoderm differentiation in combination with the AWS condition. (A) Schematic overviews of definitive endoderm differentiation conditions used in this study. (B) Summary of FACS results showing the percentages of SOX17 + FOXA2 + population acquired after 7 days of definitive endoderm differentiation of H1 and H9 hESCs under various plating densities and differentiation conditions. Results from six independent experiments ( n = 6) are shown as mean +- SD; 3-5 data points were collected and analyzed for each condition. (C) FACS analysis of SOX17 and FOXA2 in H1 hESCs treated with definitive endoderm differentiation conditions including AWS, MEG+AWS, and MEG->AWS for 7 days. Cells were plated at 1.0 x 10 5 cells/cm 2 . (D) Immunofluorescent staining of SOX17 (red) and FOXA2 (green) in H9 hESCs induced to undergo definitive endoderm differentiation under the MEG->AWS condition for 1, 3, and 7 days. Nuclei were stained with DAPI (blue). Scale bar: 100 mum. (E) Western blotting of SOX17, FOXA2, CER1, and AFP in H9 hESCs induced to undergo definitive endoderm differentiation under AWS, MEG+AWS, and MEG->AWS conditions for 7 days. Cells cultured in mTeSR1 medium were used as a control (mTeSR1). Cells were plated at 0.25 x 10 5 cells/cm 2 for the AWS condition, 1.0 x 10 5 cells/cm 2 for the MEG+AWS condition, and 1.5 x 10 5 cells/cm 2 for the MEG->AWS condition. alpha-TUBULIN was used as a loading control. (F) Quantitative-PCR analysis {TM}

Product Details

MA5-15554

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

View 1 publication 1 publication

ELISA (ELISA)

1:10,000

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

9D6

Immunogen

Purified recombinant fragment of human CER1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10985456

Product Specific Information

MA5-15554 targets CER1 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples.

The MA5-15554 immunogen is purified recombinant fragment of human CER1 expressed in E. Coli.

MA5-15554 detects CER1 which has a predicted molecular weight of approximately 30kDa.

Target Information

This gene encodes a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein antagonists that can bind directly to BMPs and inhibit their activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Clinical significance of the resistance proteins LRP, Pgp, MRP1, MRP3, and MRP5 in epithelial ovarian cancer.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society

Sedláková I,Laco J,Caltová K,Červinka M,Tošner J,Řezáč A,Špaček J

MA5-15554 was used in immunohistochemistry - paraffin section to correlate the expressions of lung resistance protein, P-glycoprotein, multidrug resistance-associated protein (MRP)-1, MRP3, and MRP5 and histopathological parameters and clinical outcome in patients with ovarian cancer

Sun Feb 01 00:00:00 UTC 2015

Mesendogen, a novel inhibitor of TRPM6, promotes mesoderm and definitive endoderm differentiation of human embryonic stem cells through alteration of magnesium homeostasis.

Heliyon

Geng Y,Feng B

MA5-15554 was used in western blot to examine the roles of TRPM6/TRPM7 channels and cellular magnesium homeostasis during development

Tue Dec 01 00:00:00 UTC 2015

Mesendogen, a novel inhibitor of TRPM6, promotes mesoderm and definitive endoderm differentiation of human embryonic stem cells through alteration of magnesium homeostasis.

Heliyon

Geng Y,Feng B

MA5-15554 was used in western blot to examine the roles of TRPM6/TRPM7 channels and cellular magnesium homeostasis during development

Tue Dec 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Cerberus; cerberus 1, cysteine knot superfamily, homolog; cerberus-related 1; Cerberus-related protein; DAN domain family member 4; MGC119894; MGC119895; MGC96951

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Gene Aliases: CER1; DAND4

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UniProt ID: (Human) O95813

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Entrez Gene ID: (Human) 9350

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Function(s)

cytokine activity morphogen activity BMP binding protein homodimerization activity

Process(es)

ureteric bud development growth plate cartilage chondrocyte proliferation gastrulation nervous system development negative regulation of cell proliferation anterior/posterior axis specification anterior/posterior pattern specification signal transduction involved in regulation of gene expression negative regulation of Wnt signaling pathway bone mineralization BMP signaling pathway negative regulation of BMP signaling pathway negative regulation of activin receptor signaling pathway sequestering of BMP in extracellular matrix cell migration involved in gastrulation determination of dorsal identity determination of heart left/right asymmetry cellular response to BMP stimulus negative regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry negative regulation of mesoderm development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CER1 Monoclonal Antibody (9D6)

CER1 Antibody (MA5-15554) in IHC (P)

Product Details

MA5-15554

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

ELISA (ELISA)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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