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Invitrogen
CES1 Monoclonal Antibody (3F10)
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FIGURE: 1 / 9
CES1 Antibody (MA5-49209) in ICC/IF
Immunocytochemistry analysis of CES1 in HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in CES1 Monoclonal antibody (Product # MA5-49209) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for ... View More
Product Details
MA5-49209
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
3F10
Immunogen
E. coli-derived human Liver Carboxylesterase 1/CES1 recombinant protein (Position: E99-A206).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074867
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human THP-1 whole cell, human HEPG2 whole cell, rat liver tissue, mouse liver tissue. IHC: human liver cancer tissue, human tonsil tissue, human prostate cancer tissue, mouse lung tissue, rat liver tissue. ICC/IF: HEPG2 cell. Flow: HEPG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ACAT; acyl coenzyme A:cholesterol acyltransferase; Acyl-coenzyme A:cholesterol acyltransferase; Brain carboxylesterase hBr1; Carboxyesterase ES-1; carboxyesterase ES-3; Carboxylesterase 1; carboxylesterase 1 (monocyte/macrophage serine esterase 1); carboxylesterase 1-like; Carboxylesterase 1C; Carboxylesterase 1E; Carboxylesterase 1G; carboxylesterase 2 (liver); CE-1; CEH; Cholesteryl ester hydrolase; Cocaine carboxylesterase; E1; Egasyn; Es-22; ES-HTEL; ES-THET; ES-x; esterase 1; Esterase 2 member of carboxylesterase-cluster 1; esterase 2, member of carboxylesterase-cluster 1; esterase 22; Esterase-2; esterase-22; HMSE; human monocyte/macrophage serine esterase 1; Liver carboxylesterase 1; Liver carboxylesterase 22; liver carboxylesterase 3; Liver carboxylesterase N; Lung surfactant convertase; Methylumbelliferyl-acetate deacetylase 1; MGC117365; Monocyte/macrophage serine esterase; Neutral retinyl ester hydrolase; NREH; PES-N; pI 5.5 esterase; REH; Retinyl ester hydrolase; Serine esterase 1; TGH; triacylglycero; Triacylglycerol hydrolase
Gene Aliases: ACAT; CE-1; CEH; Ces-1; Ces-N; CES1; Ces1c; Ces1e; Ces1g; Ces1l; CES2; Ee-1; Ee1; Eg; EG244595; egasyn; Es-22; Es-4; Es-N; Es1; Es2; Es22; Es4; EsN; Gm4976; hCE-1; HMSE; HMSE1; PCE-1; PESN; REH; Ses-1; SES1; TGH
UniProt ID: (Human) P23141, (Mouse) P23953, (Rat) P10959, (Mouse) Q64176, (Mouse) Q8VCC2
Entrez Gene ID: (Human) 1066, (Rat) 679817, (Mouse) 244595, (Mouse) 13884, (Rat) 24346, (Rat) 29225, (Mouse) 13897, (Mouse) 12623
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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