Proteintech

CFLAR/FLIP Polyclonal Antibody

CFLAR/FLIP Antibody in Immunocytochemistry (ICC/IF)

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CFLAR/FLIP Antibody (10394-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CFLAR/FLIP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CFLAR/FLIP Antibody in Western Blot (WB)

CFLAR/FLIP Antibody in Immunoprecipitation (IP)

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Product Details

10394-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CFLAR/FLIP Fusion Protein Ag0593 (31-184 aa encoded by BC001602)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.72 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DVVPPNVRD LLDILRERGK LSVGDLAELL YRVRRFDLLK RILKMDRKAV ETHLLRNPHL VSDYRVLMAE IGEDLDKSDV SSLIFLMKDY MGRGKISKEK SFLDLVVELE KLNLVAPDQL DLLEKCLKNI HRIDLKTKIQ KYKQSVQGAG TSYRN (31-184 aa encoded by B C001602)

Target Information

FLIP (CFLAR) is an apoptosis regulator protein which functions as a crucial link between cell survival and cell death pathways in mammalian cells. It acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DAB2IP Is a Bifunctional Tumor Suppressor That Regulates Wild-Type RAS and Inflammatory Cascades in KRAS Mutant Colon Cancer.

Cancer research

Miller AL,Perurena N,Gardner A,Hinoue T,Loi P,Laird PW,Cichowski K

Fri Jun 02 00:00:00 UTC 2023

Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65.

Chemico-biological interactions

Tan J,Sun M,Yin J,Zhou Q,Zhao R,Chen Q,Sun H,Jiang C,Li S,He Y

Thu Dec 01 00:00:00 UTC 2022

Cellular FLICE-like inhibitory protein (cFLIP) critically maintains apoptotic resistance in human lens epithelial cells.

Cell death & disease

Huangfu J,Hao C,Wei Z,Wormstone IM,Yan H,Fan X

Fri Apr 09 00:00:00 UTC 2021

Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.

Journal of cellular and molecular medicine

Zhang Y,Ye T,Zhou P,Li R,Liu Z,Xie J,Hua T,Sun Q

Wed Dec 01 00:00:00 UTC 2021

Regorafenib suppresses epidermal growth factor receptor signaling-modulated progression of colorectal cancer.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Liu YC,Tsai JJ,Weng YS,Hsu FT

Sat Aug 01 00:00:00 UTC 2020

Regorafenib suppresses epidermal growth factor receptor signaling-modulated progression of colorectal cancer.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Liu YC,Tsai JJ,Weng YS,Hsu FT

Sat Aug 01 00:00:00 UTC 2020

Chidamide induces necroptosis via regulation of c‑FLIPL expression in Jurkat and HUT‑78 cells.

Molecular medicine reports

Chi Z,Gao H,Liu H,Wu B,Zhang B,Gu M,Yang W

Sat Feb 01 00:00:00 UTC 2020

YM155 sensitizes HeLa cells to TRAIL-mediated apoptosis via cFLIP and survivin downregulation.

Oncology letters

Chandrasekaran AP,Poondla N,Ko NR,Oh SJ,Ramakrishna S

Thu Oct 01 00:00:00 UTC 2020

CRISPR-mediated upregulation of DR5 and downregulation of cFLIP synergistically sensitize HeLa cells to TRAIL-mediated apoptosis.

Biochemical and biophysical research communications

Poondla N,Chandrasekaran AP,Heese K,Kim KS,Ramakrishna S

Tue Apr 23 00:00:00 UTC 2019

Coordinated repression of BIM and PUMA by Epstein-Barr virus latent genes maintains the survival of Burkitt lymphoma cells.

Cell death and differentiation

Fitzsimmons L,Boyce AJ,Wei W,Chang C,Croom-Carter D,Tierney RJ,Herold MJ,Bell AI,Strasser A,Kelly GL,Rowe M

Thu Feb 01 00:00:00 UTC 2018

Coordinated repression of BIM and PUMA by Epstein-Barr virus latent genes maintains the survival of Burkitt lymphoma cells.

Cell death and differentiation

Fitzsimmons L,Boyce AJ,Wei W,Chang C,Croom-Carter D,Tierney RJ,Herold MJ,Bell AI,Strasser A,Kelly GL,Rowe M

Thu Feb 01 00:00:00 UTC 2018

Effects of histone deacetylase inhibitor trichostatin A combined with cisplatin on apoptosis of A549 cell line.

Thoracic cancer

Zhang X,Jiang SJ,Shang B,Jiang HJ

Sun Mar 01 00:00:00 UTC 2015

Effects of histone deacetylase inhibitor trichostatin A combined with cisplatin on apoptosis of A549 cell line.

Thoracic cancer

Zhang X,Jiang SJ,Shang B,Jiang HJ

Sun Mar 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: c-FLIP; CASH; CASP8 and FADD-like apoptosis regulator; Caspase homolog; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; Casper; Cellular FLICE-like inhibitory protein; CLARP; FADD-like antiapoptotic molecule 1; FLAME-1; I-FLICE; Inhibitor of FLICE; MACH-related inducer of toxicity; MRIT; testis secretory sperm-binding protein Li 233m; Usurpin; usurpin beta

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Gene Aliases: c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8AP1; Casper; CFLAR; CLARP; FLAME; FLAME-1; FLAME1; FLIP; I-FLICE; MRIT

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UniProt ID: (Human) O15519

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Entrez Gene ID: (Human) 8837

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Function(s)

protease binding death receptor binding protein binding enzyme activator activity protein complex binding cysteine-type endopeptidase activity involved in execution phase of apoptosis

Process(es)

proteolysis apoptotic process skeletal muscle tissue development skeletal muscle atrophy regulation of skeletal muscle satellite cell proliferation skeletal myofibril assembly viral process negative regulation of apoptotic process positive regulation of catalytic activity positive regulation of I-kappaB kinase/NF-kappaB signaling skeletal muscle tissue regeneration positive regulation of NF-kappaB transcription factor activity regulation of necroptotic process execution phase of apoptosis negative regulation of myoblast fusion regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of extrinsic apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CFLAR/FLIP Polyclonal Antibody

CFLAR/FLIP Antibody (10394-1-AP) in ICC/IF

Product Details

10394-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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