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Invitrogen
CFTR Monoclonal Antibody (CF3)
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FIGURE: 1 / 9
CFTR Antibody (MA1-935) in ICC/IF
Immunofluorescent analysis of CFTR using CFTR Monoclonal antibody (CF3) (Product # MA1-935) shows staining in WiDr colon carcinoma cells. CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR (Product # MA1-935) at a dilution of 1:100-1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. ... View More
Product Details
MA1-935
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
CF3
Immunogen
Synthetic Peptide: G(103) R I I A S Y D P D N K E E R(117)
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2081230
Product Specific Information
MA1-935 detects cystic fibrosis transmembrane conductance factor (CFTR) in human and mouse tissues.
MA1-935 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a single ~170 kDa protein representing CFTR in T84 whole cell extract. Immunofluorescence staining of CFTR in mouse epithelial cells with MA1-935 results in cell surface staining, consistent with localization at the plasma membrane. This antibody also detects one or more immunologically related proteins in mouse cell line Heb7a that does not contain CFTR mRNA. MA1-935 can also be used to inhibit the epithelial uptake of S. typhi in some mouse cell lines.
MA1-935 immunizing peptide corresponds to amino acid residues 103-117 found in the first extracellular loop of human and rabbit CFTR. This sequence is highly conserved in mouse, sheep, cow, and Xenopus laevis.
Target Information
Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S. typhi intestinal submucosal uptake.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATP Binding Cassette Superfamily C Member 7 (ABCC7); ATP-binding cassette sub-family C member 7; ATP-binding cassette transporter sub-family C member 7; ATP-binding cassette, subfamily c, member 7; cAMP-dependent chloride channel; CFTR; Channel conductance-controlling ATPase; Cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7); cystic fibrosis transmembrane conductance regulator homolog; cystic fibrosis transmembrane conductance regulator homolog; ATP-binding cassette, subfamily c, member 7; tcag7.78; TNR CFTR
Gene Aliases: ABC35; ABCC7; AW495489; CF; CFTR; CFTR/MRP; dJ760C5.1; MRP7; TNR-CFTR
UniProt ID: (Human) P13569, (Mouse) P26361
Entrez Gene ID: (Human) 1080, (Mouse) 12638
ATP-binding and phosphorylation-dependent chloride channel activity
chloride channel activity
channel-conductance-controlling ATPase activity
protein binding
ATP binding
bicarbonate transmembrane transporter activity
chloride transmembrane transporter activity
chloride channel regulator activity
chloride channel inhibitor activity
enzyme binding
PDZ domain binding
anion transmembrane-transporting ATPase activity
nucleotide binding
hydrolase activity
ATPase activity
ATPase activity, coupled to transmembrane movement of substances
cholesterol biosynthetic process
transport
ion transport
chloride transport
water transport
bicarbonate transport
iodide transport
cholesterol transport
transepithelial chloride transport
lung development
vasodilation
positive regulation of vasodilation
sperm capacitation
intracellular pH elevation
transmembrane transport
membrane hyperpolarization
cellular response to cAMP
chloride transmembrane transport
positive regulation of voltage-gated chloride channel activity
vesicle docking involved in exocytosis
respiratory gaseous exchange
positive regulation of insulin secretion involved in cellular response to glucose stimulus
positive regulation of exocytosis
positive regulation of cyclic nucleotide-gated ion channel activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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