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Invitrogen
CGI58 Polyclonal Antibody
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CGI58 Antibody (PA5-18666) in ICC/IF
Immunocytochemistry analysis of CGI58 using CGI58 Polyclonal Antibody (Product # PA5-18666) in paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and vesicle/ cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-18666
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (FPERPDLADQDR) corresponding to the internal amino acids of ABHD5 (aa 181-192).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10984069
Product Specific Information
This antibody is predicted to react with canine and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
Target Information
The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 1-acylglycerol-3-phosphate O-acyltransferase ABHD5; Abhydrolase domain-containing protein 5; aCGI58; CGI 58; CGI58 protein; Lipid droplet-binding protein CGI-58; MGC8731; Protein CGI-58; truncated abhydrolase domain-containing protein 5
Gene Aliases: 1300003D03Rik; 2010002J10Rik; ABHD5; CDS; CGI-58; CGI58; IECN2; IECN5; NCIE2
UniProt ID: (Human) Q8WTS1, (Mouse) Q9DBL9
Entrez Gene ID: (Human) 51099, (Mouse) 67469
lipid metabolic process
fatty acid metabolic process
phosphatidic acid biosynthetic process
phospholipid biosynthetic process
negative regulation of sequestering of triglyceride
positive regulation of triglyceride catabolic process
cell differentiation
positive regulation of lipoprotein lipase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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