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CHCHD10 Antibody (PA5-143955) in ICC/IF
Immunocytochemistry analysis of CHCHD10 in Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in CHCHD10 Polyclonal antibody (Product # PA5-143955) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropr... View More
Product Details
PA5-143955
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human CHCHD10 recombinant protein (Position: P80-P142).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075169
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hek293 whole cell, monkey heart tissue, monkey liver tissue, human HepG2 whole cell, human A549 whole cell, rat heart tissue, rat liver tissue, rat skeletal muscle tissue lytsates, mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue. IHC: human colonic adenocarcinoma tissue, human liver cancer tissue, human lung cancer tissue, human ovarian serous adenocarcinoma tissue, human gallbladder adenocarcinoma tissue, rat cardiac muscle tissue. ICC/IF: CACO-2 cell. Flow: THP-1 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
CHCHD10 encodes a mitochondrial protein that is enriched at cristae junctions in the intermembrane space. It may play a role in cristae morphology maintenance or oxidative phosphorylation. Mutations in this gene cause frontotemporal dementia and/or amyotrophic lateral sclerosis-2. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 7 and 19.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial; Nur77 downstream gene 2; Nur77 downstream protein 2; Protein N27C7-4
Gene Aliases: 1620401E04Rik; AI267078; C22orf16; CHCHD10; FTDALS2; IMMD; N27C7-4; Ndg2; SMAJ
UniProt ID: (Human) Q8WYQ3
Entrez Gene ID: (Human) 400916, (Mouse) 103172, (Rat) 361824
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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